Farklı Çözücülerin Gongolaria barbata'nın (Phaeophyceae) Antioksidan Özellikleri Üzerine Etkisi

Öz

Bu çalışmada, Gongolaria barbata'dan elde edilen alg ekstraktlarının antioksidan aktiviteleri, toplam fenolik ve flavonoid içerikleri sırasıyla farklı çözücüler kullanılarak incelenmiştir: etil asetat (AcOEt), su(W) ve fosfat tamponu (PH). Sonuçlara göre EtOH, W ve PB çözücüleri, sırasıyla test edilen iki ticari antioksidan olan BHT ve Vitamin C'den önemli ölçüde daha yüksek olduğu ve düşük IC50 değerleri ile orta düzeyde antioksidan potansiyeli gösterdiği kaydedildi. En yüksek toplam flavonoid içeriği W (6.91±0,09 mg/g Ext.) olarak tespit edildi ve PB en yüksek fenolik içeriği (2.29±0.01 mg GAE/g ext.) gösterdi. Klorofil a ve toplam karotenoid değerleri sırasıyla 40,5±1,20 ve 29,13±1,12 (mg/g DW) olarak ölçüldü. Fikoeritrin 0.002±0.001 (mg/g DW) olarak ölçülürken, fikosiyanin içeriği 0.04 ±0.01 (mg/g DW) olarak bulundu. Çalışmamızın sonucuna göre, Gongolaria barbata’nın antioksidan içerikleri, toplam fenolik ve flavonoid içerikleri incelendiğinde, fosfat tamponunun etanolün yerine kullanılabilecek bir çözücü olduğu belirlenmiştir.

Anahtar Kelimeler

• Antioksidan özellikleri
• Fenolik
• Flavonoid
• Gongolaria barbata
• Kahverengi alg

The Effect of Different Solvents on Antioxidant Properties of Gongolaria barbata (Phaeophyceae)

Öz

In this study, the antioxidant activities, total polyphenolic contents of algal extracts from Gongolaria barbata were examined by using ethyl acetate (AcOEt), water(W), and phosphate buffer (PH) as solvents. According to the results, EtOH, W, and PB showed medium antioxidant potential with low IC50 values. The highest total flavonoid content was detected in W (6.91±0.09 mg/g Ext., and PB showed the maximum phenolic content (2.29±0.01 mg GAE/g ext.). The chlorophyll a and total carotenoid contents were detected as 40.5±1.20 and 29.13±1.12 (mg/g DW), respectively. Phycoerythrin was 0.002±0.001 (mg/g DW), while phycocyanin content was found as0.04 ±0.01 (mg/g DW). Our results show that PB could be used as an extraction solvent for determining the antioxidant properties of G. barbata instead of ethanol.

Anahtar Kelimeler

• Antioxidant properties
• brown seaweed
• flavonoids
• Gongolaria barbata
• phenolics.

Kaynakça

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