The Relationships Between Blood Parameters of Liocarcinus depurator (Linnaeus, 1758) and Environmental Conditions in Çardak Lagoon (Çanakkale, Türkiye)

Abstract

In this study, the effects of environmental variables such as temperature, salinity, pH, and oxygen on blood variables of the portinid crab, Liocarcinus depurator, were determined. A total of 50 crabs were collected in Çardak Lagoon in 4 different periods; February, May, July, and November 2020. Crabs were first anesthetized on ice for 10 minutes and a 500 μL blood sample from each crab was collected from the coxae of the walking legs, using a 1 mL plastic syringe. The collected hemolymphs were then centrifuged and ELISA (Enzyme-Linked ImmunoSorbent Assay) analysis was performed. The effects of seasonal variables on blood variables were evaluated using Pearson’s correlation analysis. A negative relationship was found between temperature and ALB (albumin), while a negative relationship was found between saturated oxygen and GLU (glucose) and CHOL (cholesterol). The relationship between pH and CHOL was also negative.

Keywords

• Liocarcinus depurator
• Blood biochemistry
• Environmental variables
• Çardak Lagoon

Supporting Institution

The study was not supported by any Institution/organization.

Ethical Statement

Ethics committee approval is not required for this study.

Thanks

The author would like to thank Mr. Hasan Şenses TÜLÜMEN, one of the fishermen of Çardak Lagoon, for their help in collecting crab individuals in this study, and Mr. Sevdan YILMAZ and Mr. Seçil ACAR for their support in laboratory analysis.

Çardak Lagünü’nde (Çanakkale, Türkiye) Liocarcinus depurator (Linnaeus, 1758)’un Kan Parametreleri ile Çevresel Değişkenler Arasındaki İlişkisi

Abstract

Bu çalışmada, portunid yengeç Liocarcinus depurator ‘un kan değişkenleri üzerine sıcaklık, tuzluluk, pH ve doymuş oksijen gibi çevresel değişkenlerin etkileri belirlenmiştir. Çardak Lagünü'nde 4 farklı dönemde; Şubat 2020, Mayıs 2020, Temmuz 2020 ve Kasım 2020’ de toplam 50 yengeç bireyi toplanmıştır. Yengeçlere ilk olarak buzda 10 dk anestezi uygulandı ve 1mL’lik plastik bir şırınga kullanılarak her bir yengeçten yürüme bacakların koksalarından 500 μL kan örnekleri alınmıştır. Toplanan hemolenfler daha sonra santrifüj edilerek ELISA (enzime bağlı immünosorbent testi) analizi yapılmıştır. Mevsimsel değişkenlerin kan değişkenleri üzerindeki etkisi Pearson korelasyon analizi kullanılarak değerlendirilmiştir. Sıcaklık ile albümin (ALB) arasında negatif bir ilişki bulunurken, doymuş oksijen ile glukoz (GLU) ve kolesterol (CHOL) arasında negatif ilişki bulunmuştur. pH ile CHOL arasındaki ilişki de negatifti.

Keywords

• Liocarcinus depurator
• Kan biyokimyası
• Çevresel değişkenler
• Çardak Lagünü

Supporting Institution

Çalışma herhangi bir Kurum/Kuruluş tarafından desteklenmemiştir.

Ethical Statement

Bu çalışma için etik kurul iznine gerek yoktur.

Thanks

Yazar, bu çalışmada yengeç bireylerinin toplanmasındaki yardımları dolayısıyla Çardak Lagünü balıkçılarından Sayın Hasan Şenses TÜLÜMEN’e, laboratuvar analizlerindeki destekleri için Sayın Sevdan YILMAZ ve Sayın Seçil ACAR’a teşekkür eder.

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