DENEYSEL DİYABETİK RATLARDA İNSİZYONEL YARA İYİLEŞMESİNDE ALOE VERA’NIN ETKİNLİĞİNİN MMP-1 VE TIMP-1 YÖNÜNDEN İNCELENMESİ

Year 2023, , 204 - 209, 31.08.2023

Rohlat Seyrek , Sevda Soker , Özge Kaplan , Süreyya Özdemir Başaran , Fırat Aşır , Engin Deveci , Uğur Şeker

https://doi.org/10.36516/jocass.1268067

Abstract

Purpose: The aim of this study is to investigate the healing aspect of aloe vera in diabetes mellitus, which inhibits wound healing.
Materials and methods: Diabetes model was created with streptozotocin. At the end of the 14-day experiment, blood glucose was measured from the tail vein of animals in all groups and blood was taken from the heart and sacrificed. Histopathology and immunohistochemical statistics and evaluation were performed.
Results: Pycnosis and degeneration of epithelial cells were observed in diabetes groups. Leukocyte infiltration in the dermal papilla, degeneration of collagen fibers and an increase in the extracellular matrix were observed. It was observed that the epithelial layer in the aloe vera group was histologically close to the control group. It was observed that decreased inflammation in the dermal papilla and decreased in organized collagen fibers and vessel dilatation were observed. In the control group, MMP-1 and TIMP-1 expression were positive in the epidermis and dermis layers. In the diabetes group, weak expression of MMP-1 and TIMP-1 was observed in cells in the epidermis and dermis. The expression of MMP-1 and TIMP-1 in the surface epithelium in the aloe vera group was increased compared to the diabetes group.
Conclusion: Aloe vera accelerated cell and extracellular matrix regeneration with its anti-oxidative activity.

Keywords

Rat, diabetes, wound healing, mmp, aloe vera

Supporting Institution

dicle üniversitesi

Project Number

TIP.20.027

Effect of Aloe Vera On Mmp-1 And Timp-1 Expression on Diabetic Wound Healing

Abstract

Aim: The aim of this study is to investigate the healing aspect of aloe vera in diabetes mellitus, which inhibits wound healing.
Methods: Diabetes model was created with streptozotocin. At the end of the 14-day experiment, blood glucose was measured from the tail vein of animals in all groups and blood was taken from the heart and sacrificed. Histopathology and immunohistochemical statistics and evaluation were performed.
Results: Pycnosis and degeneration of epithelial cells were observed in diabetes groups. Leukocyte infiltration in the dermal papilla, degeneration of collagen fibers and an increase in the extracellular matrix were observed. It was observed that the epithelial layer in the aloe vera group was histologically close to the control group. It was observed that decreased inflammation in the dermal papilla and decreased in organized collagen fibers and vessel dilatation were observed. In the control group, MMP-1 and TIMP-1 expression were positive in the epidermis and dermis layers. In the diabetes group, weak expression of MMP-1 and TIMP-1 was observed in cells in the epidermis and dermis. The expression of MMP-1 and TIMP-1 in the surface epithelium in the aloe vera group was increased compared to the diabetes group.
Conclusions: Aloe vera accelerated cell and extracellular matrix regeneration with its anti-oxidative activity

Keywords

Diabetes, wound healing, MMP, Aloe Vera, TIMP-1

Project Number

TIP.20.027

References

• 1. Ceyhan Ö, S Akutay. Diyabetik Hastalarda Amputasyon Sonrası Yara İyileşmesi ve Bakım. Sakarya Tıp Dergisi, 2019;9(1):11-5. https://doi.org/10.31832/smj.496098
• 2. Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 2004;9:283-9. https://doi.org/10.2741/1184
• 3. Atkinson, M.A., G.S. Eisenbarth, and A.W. Michels, Type 1 diabetes. Lancet, 2014. 383(9911): p. 69-82. https://doi.org/10.1016/S0140-6736(13)60591-7.
• 4. ÖZKORKMAZ, E.G. and Ö. Yusuf, Yara iyileşmesi ve yara iyileşmesinde kullanılan bazı bitkiler. Türk Bilimsel Derlemeler Dergisi, 2009(2): p. 63-67.
• 5. Feily, A. and M. Namazi, Aloe vera in dermatology: a brief review. Giornale italiano di dermatologia e venereologia: organo ufficiale, Societa italiana di dermatologia e sifilografia, 2009. 144(1): p. 85-91.
• 6. Xu, W., et al., Antioxidant and anti-diabetic effects of caffeic acid in a rat model of diabetes. Tropical Journal of Pharmaceutical Research, 2020. 19(6): p. 1227-1232. https://doi.org/10.4314/tjpr.v19i6.17
• 7. Aktaş, A., et al., Granulocyte colony stimulating factor (GCSF) protected in ovarian tissues against ischemia-reperfusion injury. Journal of Drug Delivery and Therapeutics, 2022. 12(4): p. 26-30. https://doi.org/10.22270/jddt.v12i4.5538
• 8. Thu, H.E., M.H. Zulfakar, and S.F. Ng, Alginate based bilayer hydrocolloid films as potential slow-release modern wound dressing. Int J Pharm, 2012. 434(1-2): p. 375-83. https://doi.org/10.1016/j.ijpharm.2012.05.044
• 9. Şahin, E. and M. Öncel, Diyabet tanı ve takibinde geleneksel ve yeni biyokimyasal belirteçler. 2014. 10. Young, A. and C.-E. McNaught, The physiology of wound healing. Surgery (Oxford), 2011. 29(10): p. 475-479. https://doi.org/10.1016/j.mpsur.2011.06.011
• 11. Blakytny, R. and E. Jude, The molecular biology of chronic wounds and delayed healing in diabetes. Diabetic Medicine, 2006. 23(6): p. 594-608. https://doi.org/10.1111/j.1464-5491.2006.01773.x
• 12. DeClue, C.E. and L.P. Shornick, The cytokine milieu of diabetic wounds. Diabetes Management, 2015. 5(6): p. 525-537. https://doi.org/10.2217/dmt.15.44
• 13. Qiu, Z., A.H. Kwon, and Y. Kamiyama, Effects of plasma fibronectin on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats. J Surg Res, 2007. 138(1): p. 64-70. https://doi.org/10.1016/j.jss.2006.06.034
• 14. Costa, P.Z. and R. Soares, Neovascula rization in diabetes and its complications. Unraveling the angiogenic paradox. Life sciences, 2013. 92(22): p. 1037-1045. https://doi.org/10.1016/j.lfs.2013.04.001
• 15. Kunwar, R.M. and R.W. Bussmann, Ethnobotany in the nepal himalaya. Journal of ethnobiology and ethnomedicine, 2008. 4: p. 1-8. https://doi.org/10.1186/1746-4269-4-24
• 16. Mantle, D., M.A. Gok, and T. Lennard, Adverse and beneficial effects of plant extracts on skin and skin disorders. Adverse drug reactions and toxicological reviews, 2001. 20(2): p. 89-103.
• 17. Choi, S.W., et al., The wound‐healing effect of a glycoprotein fraction isolated from aloe vera. British Journal of Dermatology, 2001. 145(4): p. 535-545. https://doi.org/10.1046/j.1365-2133.2001.04410.x
• 18. Vogler, B. and E. Ernst, Aloe vera: a systematic review of its clinical effectiveness. British journal of general practice, 1999. 49(447): p. 823-828.
• 19. Atiba, A., H. Ueno, and Y. Uzuka, The effect of aloe vera oral administration on cutaneous wound healing in type 2 diabetic rats. Journal of Veterinary Medical Science, 2011. 73(5): p. 583-589. https://doi.org/10.1292/jvms.10-0438
• 20. Chithra, P., G. Sajithlal, and G. Chandrakasan, Influence of Aloe vera on the healing of dermal wounds in diabetic rats. Journal of ethnopharmacology, 1998. 59(3): p. 195-201. https://doi.org/10.1016/S0378-8741(97)00124
• 21. Trengove, N.J., et al., Analysis of the acute and chronic wound environments: the role of proteases and their inhibitors. Wound Repair and Regeneration, 1999. 7(6): p. 442-452. https://doi.org/10.1046/j.1524-475X.1999.00442.x
• 22. Yager, D.R., et al., Ability of chronic wound fluids to degrade peptide growth factors is associated with increased levels of elastase activity and diminished levels of proteinase inhibitors. Wound repair and regeneration, 1997. 5(1): p. 23-32. https://doi.org/10.1046/j.1524-475X.1997.50108.x 23. Wysocki, A.B., L. Staiano-Coico, and F. Grinnell, Wound fluid from chronic leg ulcers contains elevated levels of metalloproteinases MMP-2 and MMP-9. J Invest Dermatol, 1993. 101(1): p. 64-8. https://doi.org/10.1111/1523-1747.ep12359590 24. Muller, M., et al., Matrix metalloproteinases and diabetic foot ulcers: the ratio of MMP‐1 to TIMP‐1 is a predictor of wound healing. Diabetic Medicine, 2008. 25(4): p. 419-426. https://doi.org/10.1111/j.1464-5491.2008.02414.x