Effect of Biochanin-A on The Cerebellum in Cerebral Ischemia Reperfusion Injury

Year 2024, , 47 - 51, 11.03.2024

Öner Avınca , Özge Kaplan , Fırat Aşır

https://doi.org/10.36516/jocass.1425526

Abstract

ABSTRACT

Objective: To examine the protective effect of Biochanin A (BCA) on the cerebellum in cerebral ischemia reperfusion injury.
Material and Metodhs: 24 Wistar albino female rats were divided into 3 groups. Control, Ischemia reperfusion (IR), IR +BCA (20 mg/kg intraperitoneal) group. A micro bulldog clamp was placed on the left common carotid artery of the rats and cerebral ischemia was applied for 2 hours. After cerebral ischemia, the clamp was removed and reperfusion was performed for 24 hours. After 7 days, all rats were decapitated and the protective effects of Biochanin A on the cerebellum were evaluated by immunohistochemically analyses.
Results: In the IR group; S100 expression was also observed positive in neurons and neuroglia in the pia mater, molecular layer, Purkinje cells in the ganglionic layer and granule cells in the granular layer, and neuroglia in the white matter. In the IR+ Biochanin A group, it was observed that the immune activity in the granule cells and Purkinje cells in the granular layer was reduced compared to the IR group.
Conclusion: We suggest that Biochanin A treatment has a potential therapeutic role in alleviating inflammation in the cerebellum after cerebral ischemia reperfusion.

Keywords

Cerebral ischemia, Biochanin A, S100

SEREBRAL İSKEMİ REPERFÜZYON HASARINDA BİYOKANİN-A'NIN SEREBELLUM ÜZERİNDEKİ ETKİSİ

Abstract

ÖZET

Amaç:Serebral iskemi reperfüzyon hasarında Biochanin A'nın (BCA) serebellum üzerindeki koruyucu etkisini incelemek.
Materyal ve Metod: 24 Wistar albino dişi sıçan 3 gruba ayrıldı. Kontrol, İskemi reperfüzyon (İR), İR +BCA (20 mg/kg intraperitoneal) grubu. Sıçanların sol ana karotid arterine bir mikro bulldog klemp yerleştirildi ve 2 saat boyunca serebral iskemi uygulandı. Serebral iskemi sonrasında klemp çıkarıldı ve 24 saat boyunca reperfüzyon uygulandı. 7 gün sonra tüm sıçanlar dekapite edildi ve Biochanin A'nın serebellum üzerindeki koruyucu etkileri immünohistokimyasal analizlerle değerlendirildi.
Bulgular: IR grubunda; pia mater, moleküler tabaka, ganglionik tabakada Purkinje hücreleri ve granüler tabakada granül hücreleri ve beyaz cevherde nörogliadaki nöron ve nöroglialarda S100 ekspresyonu pozitif olarak gözlendi. IR+ Biyokanin A grubunda, granül hücrelerdeki ve granüler tabakadaki Purkinje hücrelerindeki immün aktivitenin IR grubuna kıyasla azaldığı gözlenmiştir.
Sonuç: Biochanin A tedavisinin serebral iskemi reperfüzyonundan sonra serebellumdaki enflamasyonu hafifletmede potansiyel bir terapötik rolü olduğunu düşünüyoruz.

Keywords

Serebral iskemi, Biyokanin A, S100

References

• 1.Katan M, Luft A. Global burden of stroke. Thieme Medical Publishers; 2018:208-11. https://doi.org/10.1055/s-0038-1649503
• 2.Grysiewicz RA, Thomas K, Pandey DK. Epidemiology of ischemic and hemorrhagic stroke: incidence, prevalence, mortality, and risk factors. Neurologic clinics. 2008;26(4):871-95. https://doi.org/10.1016/j.ncl.2008.07.003
• 3.Nagy Z, Nardai S. Cerebral ischemia/repefusion injury: From bench space to bedside. Brain research bulletin. 2017; 134:30-7. https://doi.org/10.1016/j.brainresbull.2017.06.011
• 4.Eltzschig HK, Eckle T. Ischemia and reperfusion-from mechanism to translation. Nature medicine. 2011;17(11):1391-401. https://doi.org/10.1038/nm.2507
• 5.Durukan A, Tatlisumak T. Acute ischemic stroke: overview of major experimental rodent models, pathophysiology, and therapy of focal cere-bral ischemia. Pharmacology Biochemistry and Behavior. 2007;87(1):179-97. https://doi.org/10.1016/j.pbb.2007.04.015
• 6.Lakhan SE, Kirchgessner A, Hofer M. Inflammatory mechanisms in is-chemic stroke: therapeutic approaches. Journal of translational medicine. 2009;7(1):1-11. https://doi.org/10.1186/1479-5876-7-97
• 7.Majid A. Neuroprotection in stroke: past, present, and future. Interna-tional Scholarly Research Notices. 2014;2014 https://doi.org/10.1155/2014/515716
• 8.Jurcau A, Simion A. Neuroinflammation in cerebral ischemia and ische-mia/reperfusion injuries: from pathophysiology to therapeutic strategies. International journal of molecular sciences. 2021;23(1):14. https://doi.org/10.3390/ijms23010014
• 9.Gilmore W, Chikritzhs T, Stockwell T, et al. Alcohol: taking a population perspective. Nature Reviews Gastroenterology & Hepatology. 2016;13(7):426-34. https://doi.org/10.1038/nrgastro.2016.70
• 10.Carvalho CA, Tirapelli DP, Rodrigues AR, et al. Morphological and im-munohistochemical analysis of apoptosis in the cerebellum of rats sub-jected to focal cerebral ischemia with or without alcoholism model. Acta Cirúrgica Brasileira. 2016; 31:629-37. https://doi.org/10.1590/S0102-865020160090000009
• 11.Dawson DA, Martin D, Hallenbeck JM. Inhibition of tumor necrosis factor-alpha reduces focal cerebral ischemic injury in the spontaneously hypertensive rat. Neuroscience letters. 1996;218(1):41-4. https://doi.org/10.1016/0304-3940(96)13116-5
• 12.Guo M-m, Qu S-b, Lu H-l, et al. Biochanin A alleviates cerebral ische-mia/reperfusion injury by suppressing endoplasmic reticulum stress-induced apoptosis and p38MAPK signaling pathway in vivo and in vitro. Frontiers in endocrinology. 2021; 12:646720. https://doi.org/10.3389/fendo.2021.646720
• 13.Wang Y, Dong X, Li Z, et al. Downregulated RASD1 and upregulated miR-375 are involved in protective effects of calycosin on cerebral ische-mia/reperfusion rats. Journal of the neurological sciences. 2014;339(1-2):144-8. https://doi.org/10.1016/j.jns.2014.02.002
• 14.Wang W, Tang L, Li Y, et al. Biochanin A protects against focal cerebral ischemia/reperfusion in rats via inhibition of p38-mediated inflammatory responses. Journal of the neurological sciences. 2015;348(1-2):121-5. https://doi.org/10.1016/j.jns.2014.11.018
• 15.Başaran SÖ, Kaplan Ö, Aşır F. Effect of Gallic Acid on Distant Organ Stomach in Intestinal Ischemia Reperfusion Injury. Journal of Drug Deliv-ery and Therapeutics. 2023;13(5):17-21. https://doi.org/10.22270/jddt.v13i5.6049
• 16.Crowe AR, Yue W. Semi-quantitative Determination of Protein Expres-sion using Immunohistochemistry Staining and Analysis: An Integrated Protocol. Bio Protoc. Dec 20 2019;9(24) doi:10.21769/BioProtoc.3465 https://doi.org/10.21769/BioProtoc.3465
• 17.Aşır F, Oğlak SC, Ağaçayak E, et al. Homeobox A Cluster 7 (HOXA7) pro-tein expression increased in the placentas of patients with preterm deliv-ery. Perinatal Journal. 2023;31(3):213-8.
• 18.Aktaş A, Aşır F, Başaran SÖ, et al. Granulocyte colony stimulating factor (GCSF) protected in ovarian tissues against ischemia-reperfusion injury. Journal of Drug Delivery and Therapeutics. 2022;12(4):26-30. https://doi.org/10.22270/jddt.v12i4.5538
• 19.Leker RR, Shohami E. Cerebral ischemia and trau ma-different etiologies yet similar mechanisms: neuroprotective opportunities. Brain Research Reviews. 2002;39(1):55-73. https://doi.org/10.1016/S0165-0173(02)00157-1
• 20.Deveci E, Aşır F, Başaran SÖ, et al. Evaluation of the Effect of Resveratrol on Hippocampus in Experimental Traumatic Brain Injury. Journal of Drug Delivery and Therapeutics. 2023;13(7):1-5. https://doi.org/10.22270/jddt.v13i7.6118
• 21.Bianchi R, Kastrisianaki E, Giambanco I, et al. S100B protein stimulates microglia migration via RAGE-dependent up-regulation of chemokine expression and release. Journal of Biological Chemistry. 2011;286(9):7214-26. https://doi.org/10.1074/jbc.M110.169342
• 22.Sun P, Li Q, Zhang Q, Xu L, et al. Upregulated expression of S100A8 in mice brain after focal cerebral ischemia reperfusion. World Journal of Emergency Medicine. 2013;4(3):210. https://doi.org/10.5847/wjem.j.issn.1920-8642.2013.03.010
• 23.Fu L, Hu J, Shao F, et al. Biochanin A, as the Lrg1/TGF-β/Smad2 pathway blockade, attenuates blood-brain barrier damage after cerebral ischemi-are-perfusion by modulating leukocyte migration patterns. Biocell. 2023;47(8) https://doi.org/10.32604/biocell.2023.028602