Thymoquinone in a Rat Model of Mesenteric Ischemia-Reperfusion Injury

Year 2024, Volume: 7 Issue: 4, 243 - 246, 31.12.2024

Gorkem Ozdemir , Necdet Fatih Yaşar , Deniz Arık , İ. Özkan Alataş

Abstract

Aim: The objective of this study was to assess the protective efficacy of thymoquinone at low and high doses against ischemic reperfusion (I/R) injury resulting from superior mesenteric artery occlusion in rats.
Materials and Methods: Thirty-five Wistar Albino rats were randomly divided into five groups: control, sham, two thymoquinone treatment groups (50 and 100 mg/kg), and a DMSO control. Intestinal injury was assessed using the Park/Chiu classification system. Blood samples were analyzed for liver enzymes (ALT, AST, ALP), kidney function markers (BUN), and other markers (LDH, phosphorus).
Results: DMSO and low dose thymoquinone groups showed significantly better Park and Chiu scores (p values were 0.015 and p=0.016 respectively) High-dose thymoquinone and DMSO groups had significantly higher ALT levels than the control. Sham, low-dose thymoquinone, and DMSO groups had significantly higher AST levels than the control. Both low-dose and high-dose thymoquinone groups had significantly higher BUN levels than the control.
Conclusion: Our study suggested that low-dose thymoquinone was more effective than high-dose thymoquinone in mitigating I/R-induced intestinal injury. High-dose thymoquinone appeared to have a detrimental effect on intestinal tissue, as evidenced by the lack of significant improvement in histopathological scores compared to the sham group.
Conclusion: Our findings suggest that thymoquinone at low doses is more effective in I/R injury than at high doses. Pure DMSO and low dose thymoquinone groups showed a similar protective effect; however pure DMSO caused more deterioration in laboratory findings than low dose thymoquinone.

Keywords

thymoquinone, reperfusion injury, mesenteric ischemia

Ethical Statement

Ethical committee approval was obtained from the Local Ethics Committee for Animal Experiments of the Rectorship of Eskişehir Osmangazi University (November 20, 2015, number: 480-2) prior to the study.

Supporting Institution

Eskişehir Osmangazi University

Project Number

number: 480-2

Thanks

The authors would like to thank to the Eskişehir Osmangazi University, Faculty of Medicine for their support to the study.

Thymoquinone in a Rat Model of Mesenteric Ischemia-Reperfusion Injury

Abstract

Keywords

thymoquinone, intestinal ischemia, reperfusion injury.

Project Number

number: 480-2

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