Effect of Hydro-Priming on Seed Germination and Early Seedling Growth in Three Cucurbit Rootstock Cultivars under Salt and Osmotic Stresses

Abstract

In this study, the effects of hydro-priming was assessed to enhance germination and seedling growth of three cucurbit rootstock cultivar (Nun 9075, Shintosa and Jumbo) seeds. Seeds were soaked at 25°C over 24 hours in the dark, hydro-primed and control seeds were then germinated at 100 mM NaCl, salt and -0.6 MPa Polyethylene glycol (PEG-6000), osmotic stress conditions. Germination percentages, mean germination time, root length, root fresh and dry weight, seedling fresh and dry weights were measured after 7 days-old seedlings. Results showed that hydro-priming increased germination percentages and seedling growth parameters under both stress conditions in all three cultivars. The findings suggested that hydro-priming can be an effective method to increase germination potential and seedling growth in rootstock cultivar seeds.

Keywords

• Seed treatments
• rootstock seed
• cucurbit
• hydro-priming
• stress conditions

Hidropriming Uygulamasının Farklı Anaç Kabak Tohumlarında Tuz ve Ozmotik Stres Altında Çimlenme ve Erken Fide Gelişimi Üzerine Etkileri

Abstract

Bu araştırmada, farklı kabak anaç çeşitlerine ait tohumlarda (Nun 9075, Shintosa ve Jumbo) hidropriming uygulamalarının tohum çimlenmesi ve fide gelişimi üzerine etkileri araştırılmıştır. Tohumlar karanlık bir ortamda 25°C sıcaklıkta 24 saat boyunca suda bekletilmiştir. Hidropriming uygulanan tohumlar ve kontrol grupları daha sonra 100 mM NaCl, tuz ve -0.6 MPa Polietilen glikol (PEG-6000) ile sağlanan osmotik stres koşullarında çimlendirilmiştir. 7 gün sonra çimlenme yüzdeleri, ortalama çimlenme hızı, kök uzunluğu, yaş ve kuru sürgün ve kök ağırlıkları ölçülmüştür. Hidropriming uygulaması sonucunda, anaç kabak çeşitlerine ait tohumların çimlenme yüzdelerinin ve fide gelişim parametrelerinin her iki stres koşulunda arttığı görülmüştür. Elde edilen bulgular hidropriming uygulamasının, kabak anacı çeşitlerinin tohumlarında çimlenme potansiyelini ve fide gelişimini arttırmada etkili bir yöntem olabileceğini göstermiştir.

Keywords

• Tohum uygulamaları
• anaç tohumlar
• kabakgiller
• hydro-priming
• stres koşulları

References

1. Demir, I., and Mavi,K. (2008). Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages Brazilian Archives of Biology and Technology 51 (5) 897-902.
2. Farooq, M., Wahıd, A., Kobayashı, N., Fujita, D., and Basra, S. M. A. (2009). Plant drought stress: effects, mechanisms and management. Agron. Sustain. Dev. 29, 185-212.
3. Yetisir, H., and Sari, N. (2003). Effect of different rootstock on plant growth yield and quality of watermelon. Australian Journal of Experimental Agriculture 43, 1269-1274.
4. Passam, H. C., Stylianou, M., and Kotsiras, A. (2005). Performance of eggplant grafted on tomato and eggplant rootstocks. European Journal of Horticultural Science 70, 130-134.
5. Mavi, K., Ermis, S., and Demir, I. (2006). The effect of priming on tomato rootstock seeds in relation to seedling growth. Asian Journal of Plant Sciences 5 (6) 940-947.
6. Schwarz, D., Rouphael, Y., Colla, G., Venema, J. H. (2010). Grafting as a tool to improve tolerance of vegetables to abiotic stresses: Thermal stress, water stress and organic pollutants. Sci Hortic 127:162–171.
7. Penella, C., Nebauer, S.G., Quinones, A., Bautista, A. S., López-Galarza, S., and Calatayud, A. (2015). Some rootstocks improve pepper tolerance to mild salinity through ionic regulation. Plant Science 230:12–22.
8. Yan, M. (2016). Hydro-priming increases seed germination and early seedling growth in two cultivars of Napa cabbage grown under salt stress The Journal of Horticultural Science and Biotechnology, 91, (4) 421-426.

9. Shukla, N., Kuntal, H., Shanker, A., and Sharma, S. (2018). Hydro-priming methods for initiation of metabolic process and synchronization of germination in mung bean (Vigna radiata L.) seeds. Journal of Crop Science and Biotechnology, 21(2), 137-146.
10. Taylor, A. G., and Harman, G. E. (1990). Concepts and Technologies of Selected Seed Treatments. Annual Review of Phytopathology, 28, 321-339.
11. Parera, C. A., and Cantliffe, D. J. (1994). Presowingseed priming Horticultural Reviews, 16, 109-139.
12. Sung, J. M., and Chiu, K. Y. (1995). Hydration effect on seedling emergence strength of watermelon seeds differing in ploidy. Plant Science, 110, 21-26.
13. ISTA. (2019). International Rules for Seed Testing, International Seed Testing Association Bassersdorf, Switzerland.
14. Ibrahim, E. A. (2016). Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology, 192, 38-46.
15. Ermis, S., Ozden, E., Njie, E. S., and Demir, I. (2016). Pre-treatment germination percentages affected the advantage of priming treatment in pepper seeds. American Journal of Experimental Agriculture, 13(1), 1-7.
16. Taylor, A. G., Allen, P. S., Bennett, M. A., and Bradford, K. J. (1998). Seed enhancement. Seed Science Research, 8, 245-256.
17. Demir, I., Ermis, S., Mavi, K., and Matthews, S. (2008). Mean germination time of pepper seed lots (Capsicum annuum L.) predicts size and uniformity of seedlings in germination tests and transplant modules. Seed Science and Technology, 36, 21-30.
18. Taylor, A. G., and Ten Broeck. C. W. (1988). Seedling emergence forces of vegetable crops. HortScience, 23:367.
19. Eneas Filho, J., Oliveira Neto, O. B., Prisco, J. T., Gomes Filho, E., and Monteiro, C. (1995). Effects of salinity in vivo and in vitro on cotyledonary galactosidases from Vigna unguiculata (L.) Walp. during seed germination and seedling establishment. Revista Brasilieria de Fisiologia Vegetal, 7 (2), 135-142.
20. Maiti, R., Pramanik, K., (2013). Vegetable seed priming: a low cost, simple and powerful techniques for farmers’ livelihood. Int. J. Bio-Resour. Stress Manag, 4, 475-481.