Roselle bitkilerini enfekte eden Cotton leaf curl Multan virüsünün (CLCUMUV) moleküler karakterizasyonu ve evrimsel geçmiş analizi

Abstract

Roselle (Hibiscus sabidarrifa, Hibiscus esculentus) bitkileri, çok yönlü kullanımları için birçok tropikal ve subtropikal iklimde yetiştirilmektedir. Bitki virüsleri, tarımsal üretimde maksimum kayıplara neden olma konusunda önemli bir kapasiteye sahiptir. Dünya genelinde gıda güvenliği veya ekolojik bütünlük için büyük tehdit oluşturan bitki hastalıklarının yaklaşık yarısının ortaya çıkmasından bu virüslerin sorumlu olduğu varsayılmaktadır. Geminivirüsler, karakteristik ikiz yarı-ikosahedral viryonlara ve küçük, tek sarmallı dairesel bir DNA genomuna sahip bitki virüsleri ailesidir. Begomovirüslerin karakteristik semptomlarını gösteren bazı Roselle bitkileri gözlemlendi. Enfekte bitkilerden 550 bp'lik bir amplikon boyutu elde edildi. Sekans analizi, bu semptomatik bitkilerde Cotton leaf curl Multan virüsünün varlığını kanıtlamıştır. 2.4-2.5 kb tam uzunlukta bir genom boyutu elde edildi. Bir filogenetik analiz ve evrimsel tarih analizi, bu virüsün diğer hizalanmış virüslerle ilişkisini doğrulamıştır. Genel olarak, belirli bir bölgede yeni bir begomovirüs konakçısı belirledik.

Keywords

• Roselle
• Begomovirus
• AC2 geni
• Filogenetik analiz
• evrimsel tarih
• nükleotit karşılaştırması

The Molecular Characterization and Evolutionary History Analysis of Cotton leaf curl Multan virus (CLCUMUV) Infecting (Hibiscus sabidarrifa, Hibiscus rosa-sinensis) in Punjab Province of Pakistan

Abstract

The plants (Hibiscus sabidarrifa and Hibiscus rosa-sinensis) are cultivated in many tropical and subtropical climates for their versatile usage. Plant viruses have a significant capacity to cause the maximum losses in agricultural production. It is assumed that these viruses are responsible for the emergence of about half of plant diseases which are a major threat to food safety or ecological integrity worldwide. Geminiviruses are family of plant viruses with characteristic twinned quasi-icosahedral virions and a small, single-stranded circular DNA genome. Some plants (H. sabidarrifa and H. rosasinensis) showing the characteristic symptoms of viruses were observed. The presence of whitefly vector presumed that these plants might have begomovirus. A 550 bp of amplicon size as a plasmid was obtained from the infected plants after PCR reaction. The obtained amplicon was amplified with sequence specific primers to get the full length genome. The sequence analysis has proved the existence of Cotton leaf curl Multan virus (CLCUMUV) from symptomatic plants. The full genome sequence analysis revealed a 2.4-2.5 kb full genome of CLCUMUV infecting Roselle species. A phylogenetic and evolutionary history analysis has confirmed the association of this virus with other aligned viruses. Overall, we have identified one new species as host of begomovirus in this area.

Keywords

• Roselle
• Begomovirus
• AC2 gene
• Phylogenetic analysis
• nucleotide comparison

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