Tıbbi Nane (Mentha piperita L.) Bitkisinin Fide Gelişim Döneminde Uygulanan Farklı BAP, IBA ve IAA Hormon Dozlarının Büyüme ve Biyokimyasal Parametreler Üzerine Etkileri

Abstract

Bu çalışma, Mentha piperita L. bitkisinde BAP, IBA ve IAA hormonlarının 50 ve 100 ppm dozlarının büyüme ve biyokimyasal parametreler üzerindeki etkilerini değerlendirmek amacıyla gerçekleştirilmiştir. Araştırma, ‘‘Şansa Bağlı Tesadüf Parselleri’’ desenine göre sera koşullarında üç tekrar ile yapılmıştır. İncelenen parametreler arasında fide ve kök uzunlukları, fide ve kök yaş ile kuru ağırlıkları, toplam fenolik madde miktarı ve antioksidan aktivite (CUPRAC ve FRAP) bulunmaktadır. Çalışma sonucunda; fide kuru ağırlığı dışındaki büyüme parametrelerinde hormon uygulamalarının kontrole göre önemli etkisinin olduğu, fide uzunluk, fide yaş ağırlık ve kök kuru ağırlıklarında en yüksek değerler BAP50 uygulamalarından, kök uzunluğunda en yüksek değer IBA50, kök yaş ağırlığında ise IAA50 uygulamalarında tespit edilmiştir. Uygulanan sentetik biyostimülantların antioksidan aktiviteler ile toplam fenolik madde miktarı üzerine önemli bir etkisinin olmadığı görülmüştür.

Keywords

• Antioksidan
• Biyostimülan
• Fide Gelişim
• Tıbbi nane
• Toplam Fenolik Madde

Effects of Different BAP, IBA, and IAA Hormone Doses on Growth and Biochemical Parameters during the Seedling Development Period of Medicinal Mint (Mentha piperita L.) Plant

Abstract

This study was conducted to evaluate the effects of BAP, IBA, and IAA hormones at doses of 50 and 100 ppm on growth and biochemical parameters in Mentha piperita. The research was carried out under greenhouse conditions with three replications, following a ‘‘Completely Randomized Experimental Design’’. The parameters examined included seedling and root lengths, seedling and root fresh and dry weights, total phenolic content, and antioxidant activity (CUPRAC and FRAP). The results indicated that, except for seedling dry weight, hormone applications had significant effects on growth parameters compared to the control. The highest values for seedling length, seedling fresh weight, and root dry weight were observed with BAP50 treatments, the highest root length was with IBA50, and the highest root fresh weight was with IAA50 treatments. It was found that the applied synthetic biostimulants had no significant effect on antioxidant activities and total phenolic content.

Keywords

• Antioxidant
• Biostimulant
• Seedling Development
• Medicinal Mint
• Total Phenolic Content

References

1. [1] Singh, R., Shushni, A.M.M., & Belkheir, A. (2015). Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry, 8, 322–328.
2. Benabdallah, A., Boumendjel, M., Aissi, O., Rahmoune, C., Boussaid, M., & Messaoud, C. (2018). Chemical composition, antioxidant activity and acetyl cholinesterase inhibitory of wild Mentha species from Northeastern Algeria. South African Journal of Botany, 116, 131–139.
3. Kumar, P., Mishra, S., Malik, A., & Satya, S. (2011). Insecticidal properties of Mentha species: A review. Industrial Crops and Products, 34, 802–817.
4. Mahendran, G., & Rahman, L.U. (2020). Ethnomedicinal, phytochemical and pharmacological updates on Peppermint (Mentha× piperita L.) A review. Phytotherapy Research, 34(9), 2088-2139.
5. Patrick, D.J. (2015). Plant biostimulants: definition, concept, main categories and regulation. Scientia Horticulture. 196, 3 - 14.
6. Mayerni, R., Eka Pratiwi, E., & Warnita, W. (2015). Shoot multiplication of quinine plant (Cinchona ledgeriana Moens) with several concentrations of kinetin on in vitro. International Journal on Advanced Science, Engineering and Information Technology, 5(2), 57-61.
7. Rulcová, J., & Pospíšilová, J. (2001). Effect of benzylaminopurine on rehydration of bean plants after water stress. Biologia Plantarum, 44, 75-81.
8. Taiz, L., and Zeiger, E. (2010). Plant physiology (5th ed. Sinauer Associates, Inc, Massachusetts).

9. Nourafcan, H., Sefidkon, F., Khalighi, A., Mousavi, A., Sharifi, M. (2014). Efects of IAA and BAP on chemical composition and essential oil content of lemon verbena (Lippia citriodora H.B.K). J Herb Med 5:25–32
10. Hemmati, N., Cheniany, M., & Ganjeali, A. (2020). Effect of plant growth regulators and explants on callus induction and study of antioxidant potentials and phenolic metabolites in Salvia tebesana Bunge. Botanica serbica, 44(2), 163-173.
11. Yang, Y., Wang, Q.L., Geng, M.J., Guo, Z.H. and Zhao, Z. (2011). Effect of Indole-3-Acetic Acid on Aluminum-Induced Efflux of Malic Acid from Wheat (Triticum aestivum L.). Plant and Soil, 346: 215–230.
12. Sevik, H., and Guney, K. (2013). Effects of IAA, IBA, NAA, and GA3 on rooting and morphological features of Melissa officinalis L. stem cuttings. ScientificWorldJournal 2013:909507. doi: 10.1155/2013/909507
13. Kumlay, A.M., & Eryiğit, T. (2011). Bitkilerde büyüme ve gelişmeyi düzenleyici maddeler: bitki hormonları. Journal of the Institute of Science and Technology, 1(2), 47-56.
14. Anonim (2024) https://www.accuweather.com/tr/tr/arifbey/320558/november-weather/320558?year=2023 Erişim Tarihi: 02/08/2024
15. Waterhouse, A.L. (2002). Determination of total phenolics. Current protocols in food analytical chemistry, 6(1), I1-1.
16. Sachett, A., Gallas-Lopes, M., Conterato, G.M.M., Herrmann, A.P., & Piato, A. (2021). Antioxidant activity by FRAP assay: in vitro protocol. Protocols, http://dx.doi.org/10.17504/protocols.io.btqrnmv6
17. Ak, T., & Gülçin, I. (2008). Antioxidant and radical scavenging properties of curcumin. Chemico-biological interactions, 174(1), 27-37.
18. Lee, H.B., Im, N.H., An, S.K., & Kim, K.S. (2021). Changes of growth and inflorescence initiation by exogenous gibberellic acid3 and 6-benzylaminopurine application in Phalaenopsis orchids. Agronomy. 11(2), 196.
19. Shekhawat, M.S., Kannan, N., Manokari, M., Revathi, J. (2012). In vitro propagation of Oldenlandia umbellata L.—a highly medicinal & dye-yielding plant of coromandel coast. Int J Recent Sci Res 3(9):758–761
20. Krishnan, S.R.S., Siril, E.A. (2017). Enhanced In Vitro Shoot Regeneration in Oldenlandia umbellata L. by Using Quercetin: A Naturally Occurring Auxin-Transport Inhibitor. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci, 87(3), 899–904.
21. Deng, Z.C., Jin, H., He, H. (2015). An efficient micropropagation system for Morinda officinalis How. (Rubiaceae). an endangered medicinal plant. J Agric Sci Tech 17:1609–1618
22. Sarker, B.C., Talukder, M., and Roy, B. (2021). Chlorophyll synthesis. growth and yield performance of summer mung bean CV. BARI MOOG-6 in response to BAP and NAA. Bangl. J. Bot. 50. 209–217.
23. Taiz, L., Zeiger, E., Møller, I.M., Murphy, A. (2015). Plant physiology and development, 6th edn. Sinauer Associates Inc, Publishers, Sunderland
24. Pei, S., Qiu-Ying, T., Jie, C., Wen-Hao, Z., (2010). Aluminium-induced inhibition of root elongation in Arabidopsis is mediated by ethylene and auxin. Journal of Experimental Botany 61:347–356.
25. Luo, J., Zhou, J.J., Zhang, J.Z., (2018). Aux/IAA gene family in plants: molecular structure, regulation, and function. International Journal of Molecular Sciences 19:259
26. Anfang, M., Shani, E. (2021). Transport mechanisms of plant hormones. Current Opinion in Plant Biology 63:102055.
27. Akbulut, M., Bakoğlu, N., Baykal, H., & Şavşatlı, Y. (2015). Maviyemişlerde (Vaccinium corymbosum L.) çelikle üretimde farklı hormon dozlarının köklenme üzerine etkisinin incelenmesi. Tarım Bilimleri Araştırma Dergisi, 8(2), 52-56.
28. Ali, B., Hayat, S., Hasan, S.A. and Ahmad, A. (2008). A comparative effect of IAA and 4-Cl-IAA on growth, nodulation and nitrogen fixation in Vigna radiata (L.) Wilczek. Acta Physiologiae Plantarum, 30(1), 35-41.
29. Zhu, C., Jiang, R., Wen, S., Xia, T., Zhu, S., & Hou, X. (2023). Foliar spraying of indoleacetic acid (IAA) enhances the phytostabilization of Pb in naturally tolerant ryegrass by limiting the root-to-shoot transfer of Pb and improving plant growth. PeerJ, 11, e16560.
30. Bashir, Z., Hussain, K., Iqbal, I., Nawaz, K., Siddiqi, E.H., Javeria, M., ... & Ali, S.S. (2021). Improvements of crop productivity in wheat (Trıtıcum aestıvum L.) by the applications of phytohormones. Pak. J. Bot, 53(2), 585-595.
31. Lin, L., Ma, Q., Wang, J., Lv, X., Liao, M. A., Xia, H., ... & Liang, D. (2018). Effects of indole‐3‐butytric acid (IBA) on growth and cadmium accumulation in the accumulator plant Stellaria media. Environmental Progress & Sustainable Energy, 37(2), 733-737.
32. Redhwan, A., Acemi, A., & Özen, F. (2023). Effects of plant growth regulators on in vitro seed germination, organ development and callogenesis in Pancratium maritimum L. Plant Cell, Tissue and Organ Culture (PCTOC), 154(1), 97-110.