Antagonistic effects of Trichoderma spp. isolated from Düzce agricultural soils on Fusarium oxysporum f. sp. lycopersici, the causative agent of tomato root and crown rot, under in vitro conditions

Abstract

In this study, the antagonistic activities of Trichoderma spp. isolated from Düzce agricultural soils against Fusarium oxysporum f. sp. lycopersici (FOL), which causes collar and root rot of tomato, were investigated under in vitro conditions. In a quadruplicate culture test of antagonist candidate Trichoderma isolates isolated from 120 soil samples taken from agricultural fields, 10 isolates were determined to have antagonistic activity against FOL In dual culture tests, while isolates GYO1F1 and GY1B1-0 showed the highest antagonistic effect against mycelial growth of the FOL at 100% and 71.87%, respectively, followed by isolates GY1F1-0, GY2F2-0, KTM1-0, GK2F2-1, GY12F2-0, and GYO2F2-0 with antagonistic effects ranging from 10.9% to 31.06%. However, isolate KT1M1-2 exhibited the lowest antagonistic activity (3.26%). The volatile compounds of isolates GYO1F1 and GY1B1-0 inhibited the colonial growth of FOL by 13.37% and 38.45%, respectively, on the sixth day after inoculation using the dual Petri dish method. In the study, it was concluded that GYO1F1 and GY1B1-0 isolates, identified as T. virens by macroscopic and microscopic morphological identification, could be promising biological control agents against FOL.

Keywords

• Tomato
• Fusarium oxysporum f.sp. lycopersici
• Trichoderma spp.
• biologycal control

Supporting Institution

This study is supported by Düzce University Research Fund

Project Number

This study is supported by Düzce University Research Fund Project Number: 2024.11.02.1509

Ethical Statement

Named “Antagonistic effects of Trichoderma spp. isolated from Düzce agricultural soils on Fusarium oxysporum f. sp. lycopersici, the causative agent of tomato root and crown rot, under in vitro conditions” has been installed in the system to be published in the Journal of Agricultural Technology. This article has not been submitted for another publication and is not in the evaluation phase.

Thanks

The authors would like to express their gratitude to the Düzce University Scientific Research Projects Coordination Office for their support.

Düzce tarım topraklarından izole edilen Trichoderma türlerinin, in vitro koşullar altında domates kök ve Kökboğazı çürüklüğüne neden olan Fusarium oxysporum f. sp. lycopersici'ye karşı antagonistik etkileri

Abstract

Bu çalışmada Düzce tarım topraklarından izole edilen Trichoderma spp.’nin domateste kök ve kök çürüklüğüne neden olan Fusarium oxysporum f. sp. lycopersici (FOL)’ye antagonistik etkileri in vitro koşullarda araştırılmıştır. Tarım alanlarından alınan 120 toprak örneğinden izole edilen antagonist adayı Trichoderma izolatlarının FOL’ye karşı dörtlü kültür denemelerinde, 10 izolatın antagonistik etki gösterdiği tespit edilmiştir. Bu izolatlarla ve FOL ile ikili kültür denemelerinde GYO1F1 ve GY1B1-0 izolatları patojenin miselyal gelişmesine karşı en yüksek antagonistik etkiyi (sırasıyla %100 ve %71.87) gösterirken, bunları %10.9-%31.06 arasında antagonistik etkiyle GY1F1-0, GY2F2-0, KTM1-0, GK2F2-1, GY12F2-0, GYO2F2-0 izolatları izlemiştir. KT1M1-2 izolatı ise en düşük antagonistik etkiyi (%3.26) göstermiştir. GYO1F1 ve GY1B1-0 izolatlarının uçucu yağları ikili petri yönteminde FOL’nin koloniyal gelişimini inokulasyondan sonra altıncı günde sırasıyla ortalama %13.37 ve %38.45 oranında inhibe ettikleri tespit edilmiştir. Çalışmada izolatlarının makroskopik ve mikroskopik morfolojik tanılarında T. virens oldukları tespit edilen GYO1F1 ve GY1B1-0 izolatları FOL’e karşı biyolojik mücadelede ümitvar oldukları sonucuna varılmıştır.

Keywords

• Domates
• Fusarium oxysporum f. sp. lycopersici
• Trichoderma spp.
• biyolojik mücadele

Supporting Institution

Düzce Üniversitesi

Project Number

This study is supported by Düzce University Research Fund Project Number: 2024.11.02.1509

Thanks

Düzce Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü'ne destelerinden ötürü teşekkürü borç biliriz.

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