The co-evaluation of endosalpingeal karyorrhexis and salpingitis after the erythropoietin effect on fallopian ischemia reperfusion injury

Year 2018, , 65 - 68, 01.05.2018

Tsompos Constantinos , Panoulis Constantinos Toutouzas Konstantinos Triantafyllou Aggeliki C. Zografos George Papalois Apostolos Kalliopi Tsarea Maria Karamperi

https://doi.org/10.28982/josam.400277

Abstract

Aim: This study
co-evaluated the 2 quoted histologic variables after the cytokine erythropoietin (Epo) administration. The calculation was based on the results of 2
preliminary studies, each one evaluating a respective histologic variable of endosalpingeal karyorrhexis (EK) or salpingitis (S) in an induced ischemia
reperfusion (IR) animal experiment.

Methods: The 2 main experimental endpoints at which the EK
and S scores were evaluated, were the
reperfusion 60^thmin
(for A & C groups) and the
reperfusion 120^thmin (for B & D groups). Specially, the groups A and B were
processed without drugs, whereas the groups C
and
D after Epo administration.

Results: The
first preliminary study showed that Epo hardly non significantly increased the
EK scores by the grade “without lesions” 0.0181818 [-0.0679319 - +0.1042955]
(p-value=0.6715). The other preliminary study found that Epo did not influence the
S scores (p-value=1.0000). Both studies were co-estimated since they belong to
the same experimental setting. This study co-evaluated the combined diagnostic
values of both variables together.

Conclusions: Epo
again hardly non significantly increased both scores for these histologic
parameters at  the grade of “without
lesions” 0.0090909 [-0.0339659 - +0.0521478] (p-value=0.6715) since they were
co-evaluated together.

Keywords

Ischemia, Erythropoietin, Endosalpingeal karyorrhexis, Salpingitis, Reperfusion

Endosalpingeal karyorrhexis ve salpingitis 'nin eritropoietin etkisinin ardından ortak değerlendirilmesi

Abstract

Amaç: Bu çalışma sitokin eritropoietin (EPO) uygulamasından sonra 2 atfedilen histolojik değişkenleri ortaklaşa değerlendirdi. Hesaplama, her biri endosalpingeal karyorrhexis (EK) veya salpingitis (S) bir indüklenen iskemi reperfüzyonu (IR) hayvan denemesi içinde ilgili histolojik değişken değerlendiren 2 ön çalışmanın sonuçlarını temel aldı.

Yöntemler: EK ve S puanlarının değerlendirilmesi gereken 2 ana deneysel uç noktası reperfüzyon 60 dk (A & C grupları için) ve reperfüzyon 120 dk (B & D grupları için) idi. Özel olarak, A ve B grupları ilaç olmadan işlendi, oysa C ve D EPO uygulamasından sonra işlendi. 

Bulgular: İlk ön çalışmada EPO çok önemli ölçüde anlamlı "lezyonlar olmadan" 0,0181818 [-0,0679319-+ 0,1042955] (p-değer = 0,6715) notu tarafından ek puanları artış gösterdi. Diğer ön çalışmada EPO, S puanlarını etkilemez bulundu (p-değer = 1,0000). Her iki çalışmada aynı deneysel ayarlara sahip olduğundan birlikte tahmin edilmiştir. Bu çalışma her iki değişkenin kombine tanı değerlerini birlikte değerlendirir. 

Sonuç: EPO yine de çok önemli ölçüde bu histolojik parametreler için "lezyonlar olmadan" 0,0090909 [-0,0339659-+ 0,0521478] (p-değer = 0,6715) sınıfında birlikte değerlendirildiğinde her iki skoru artmıştır.

Keywords

İskemi, Eritropoietin, Endosalpingeal karyorrhexis, Salpenjit, Reperfüzyon

References

• 1. Tsompos C, Panoulis C, Toutouzas K, Agrogiannis G, Patsouris E, Zografos G, Papalois A. The effect of erythropoietin on endosalpingeal karyorrhexis during ischemia reperfusion injury in rats. Acta Angiol 2014;20(3):119–23.
• 2. Tsompos C, Panoulis C, Τοutouzas K, Ζografos G, Papalois A. The Effect of Erythropoietin on Salpingitis during Ischemia Reperfusion Injury in Rats. Austin J Reprod Med Infertil. 2015;2(5):1027.
• 3. Adamyan LV, Myinbayev OA, Kulakov VI. Use of fibrin glue in obstetrics and gynecology: a review of the literature. Int J Fertil. 1991 Mar-Apr;36(2):76-7:81-8.
• 4. Castadot RG. Oral contraception in 1983 (author's transl). Contracept Fertil Sex (Paris). 1982 Nov;10(11):753-7.
• 5. Guennoun A, Krimou Y, Mamouni N, Errarhay S, Bouchikhi C, Banani A. Normal adnexal torsion and pregnancy: about a case. Pan Afr Med J. 2017 Jul 14;27:197.
• 6. Çılgın H, Şimşek M, Bal R. Can adnexal torsion be predicted by measuring plasma heat shock protein 70 level? An experimental study. Arch Gynecol Obstet. 2017 Nov;296(5):941-6.
• 7. Ayachi A, Blel Z, Khelifa N, Mkaouer L, Bouchahda R, Mourali M. Adnexal torsion during the second trimester of pregnancy: about two cases. Pan Afr Med J. 2016 Oct 25;25:113.
• 8. Sukkong K, Sananpanichkul P, Teerakidpisan P, Bhamarapravatana K, Suwannarurk K. High Rate of Gangrenous Adnexal Torsion: Dilemma of a Missing Silent Cancer. Asian Pac J Cancer Prev. 2016 Nov 1;17(11):4981-4984.
• 9. Lee MH, Meyers N, Raptis CA, Mellnick VM. Interobserver reliability for computed tomography findings of adnexal torsion. Emerg Radiol. 2017 Feb;24(1):21-24.
• 10. Damasceno RW, Avgitidou G, Belfort R Jr, Dantas PE, Holbach LM, Heindl LM. Eyelid aging: pathophysiology and clinical management. Arq Bras Oftalmol. 2015 Sep-Oct;78(5):328-31.
• 11. Spinelli C, Piscioneri J, Strambi S. Adnexal torsion in adolescents: update and review of the literature. Curr Opin Obstet Gynecol. 2015 Oct;27(5):320-5.
• 12. Tunç SY, Ağaçayak E, Yaman NS, Deveci E, Kalkanlı S, Özler A. Effects of adnexal torsion on the Fallopian tube in rats: a histologic and immunohistochemical study. Anal Quant Cytopathol Histpathol. 2014 Oct;36(5):285-9.
• 13. Türk E, Karaca İ, Ozcinar E, Celebiler A, Aybek H, Ortac R, Güven A. The effect of hypothermia on adnexal torsion/detorsion injury in a rat ovary model. J Pediatr Surg. 2015 Aug;50(8):1378-81.
• 14. Calis P, Bozdag G, Karakoc Sokmensuer L, Kender N. Does ischemia-reperfusion injury affect ovarian reserve and follicle viability in a rat model with adnexal torsion? Eur J Obstet Gynecol Reprod Biol. 2015 Feb;185:126-30.
• 15. Navve D, Hershkovitz R, Zetounie E, Klein Z, Tepper R. Medial or lateral location of the whirlpool sign in adnexal torsion: clinical importance. J Ultrasound Med. 2013 Sep;32(9):1631-4.
• 16. Sánez HA, Taboada-Pérez GC, Hernández-Arroyo L, Mateo-Madrigal M, Mateo-Madrigal V. Adnexal torsion: three cases. Ginecol Obstet Mex. 2013 May;81(5):272-8.
• 17. Hirth D, McClain SA, Singer AJ, Clark RA. Endothelial necrosis at 1 hour postburn predicts progression of tissue injury. Wound Repair Regen. 2013 Jul-Aug;21(4):563-70.
• 18. Ozler A, Turgut A, Soydinç HE, Sak ME, Evsen MS, Alabalik U, Basarali MK, Deveci E. The biochemical and histologic effects of adnexal torsion and early surgical intervention to unwind detorsion on ovarian reserve: an experimental study. Reprod Sci. 2013 Nov;20(11):1349-55.
• 19. Tsompos C, Panoulis C, Toutouzas K, Triantafyllou A, Zografos G, Papalois A. The effect of erythropoietin on chloride levels during hypoxia reoxygenation injury in rats. Signa Vitae 2017;13(2):97-101.