Taxifolinin biyokimyasal ve histopatolojik değerlendirilmesi: Rat modelinde karaciğer iskemi reperfüzyon hasarı üzerine deneysel çalışma

Öz

Amaç: Karaciğer rezeksiyonu ve transplantasyon sırasında uygulanan iskemi reperfüzyon (I/R) prosedürü karaciğerde oksidatif hasara yol açmaktadır. Bu çalışmanın amacı ratlarda iskemi reperfüzyonun neden olduğu karaciğer hasarına taxifolinin etkisini incelemektir. Yöntemler: Albino Wistar erkek ratlar her grupta 6 rat olmak üzere üç gruba ayrıldı: karaciğer iskemi reperfüzyon (LIR), 50 mg/kg taxifolin + karaciğer iskemi reperfüzyon hasarı (TLIR) ve sham operasyon (SHAM). Thiopental sodium anestezisinden bir saat önce TLIR grubuna 50 mg/kg taxifolin; LIR ve SHAM grubuna ise distile su uygulandı. TLIR ve LIR gruplarında hepatik arter, portal ven ve safra kanalına bir saat iskemi ve altı saat reperfüzyon oluşturmak üzere klemp yerleştirildi. SHAM grubunda herhangi bir prosedür uygulanmadan karın boşluğu cerrahi dikişle kapatıldı. Bu periyodun sonunda ratlar yüksek doz anestezi ile sakrifiye edildi. Karaciğer dokuları biyokimyasal ve histopatolojik inceleme için çıkarıldı. I/R prosedüründe SHAM grubu ile kıyaslandığında karaciğer dokusunda MDA (P<0,001), AST, ALT (P<0,001) değerlerinde anlamlı artış ve tGSH (P<0,001) değerinde azalma görüldü. Taxifolin uygulanan grupta bu etki baskılandı.

Bulgular: I/R grubunda patolojik olarak dilate konjeste kan damarları, hemoraji, karaciğer parankiminde destrüksiyon ve ödem gözlendi. Taxifolin uygulanan grupta karaciğer dokusu orta derecede sinusoidal konjesyon dışında normale yakın görünümdeydi.

Sonuç: Sonuçlarımız taxifolinin iskemi reperfüzyon sonrası oluşan karaciğer hasarını önlemede etkili olduğunu göstermektedir.

Anahtar Kelimeler

• İskemi,Reperfüzyon,Rat,Taxifolin

Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury

Abstract

Aim: Ischemia/reperfusion (I/R) procedure applied during liver resection and transplantation in clinic settings causes liver oxidative damage. The aim of this study was to examine the effects of taxifolin on liver injury induced by I/R in rats.

Methods: Albino Wistar male rats divided into three groups with 6 rats in each group: liver ischemia-reperfusion (LIR), 50 mg/kg taxifolin + liver ischemia reperfusion (TLIR) and sham operation (SHAM). An hour before thiopental sodium anesthesia, 50 mg/kg taxifolin was orally administered to the TLIR group and distilled water to the LIR and SHAM groups. In the TLIR and LIR groups, a clamp was placed in the hepatic artery, portal vein and bile duct, thereby inducing ischemia for one hour, and reperfusion for six hours. In the SHAM group, the abdominal cavity was closed by surgical suture without any procedure. At the end of this period, rats were sacrificed with high dose anesthesia. Liver tissues were removed for biochemical and histopathological examinations

 Results: I/R procedure significantly increased MDA (P<0.001), ALT, AST levels (P<0.001) and decreased tGSH level (P<0.001) in liver tissue compared to SHAM group. In the taxifolin treated group, this effect was suppressed. Histopathologically, in the I/R induced group, pathological findings such as dilated congested blood vessel, hemorrhage, destruction in the liver parenchyma and edema observed. Liver tissue in the taxifolin group had near-normal appearance except mild sinusoidal congestion.

Conclusion: Our results indicate that taxifolin is an effective agent in reducing hepatic damage caused by I/R.

Keywords

• Ischemia,Reperfusion,Rat,Taxifolin

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