Determination of In Vitro and In Vivo Efficacy of Some Bacterial Antagonists Against Sclerotinia sclerotiorum (Lib.) De Bary in Sunflowers

Year 2024, , 362 - 374, 13.03.2024

Raziye Koçak , Nuh Boyraz

https://doi.org/10.33462/jotaf.1259380

Cited By: 1

Abstract

This study was carried out in 2017-2018 to determine the in vitro and in vivo activities of some bacterial bioagents against Sclerotinia sclerotiorum, which causes root and root-collar rot in sunflower cultivation areas of Konya and Aksaray provinces. Against the two most virulent S. sclerotiorum (Hırkatol and Eskil) isolates selected as a result of pathogenicity tests after being isolated and diagnosed from diseased plants which were collected from sunflower cultivation areas in Konya and Aksaray provinces, the antifungal effects of 16 bacterial isolates from the soil in the rhizosphere region of the healthy sunflower plants from the same areas were evaluated. Primarily, the most effective bacterial bioagents were determined by dual culture tests. As a result of the in vitro tests, a total of 5 bacterial isolates constituting the largest zone diameter were molecularly identified according to 16S rRNA and were used in pot experiments. The bacteria were identified as Bacillus cereus, Bacillus simplex, Brevibacterium frigoritolerans, Bacillus toyonensis (2 isolates) and were coded using the BLAST program of the GenBank database (NCBI). As per in vitro, the highest effect in both isolates of S. sclerotiorum was observed in Bacillus cereus and Bacillus simplex with an inhibition rate of 49.19-57.95%. Except for Bacillus toyonensis (B1), one of the bacterial species which were tested in vivo, all the bacteria reduced or stopped lesion development compared to the control. As a result of the application, the biological control agent completely prevented the growth of both the isolates of Bacillus cereus and Bacillus simplex S. sclerotiorum in in vivo conditions (100%). Efficacy studies have shown that bacterial isolates both cause healthy growth of sunflower plants and significantly prevent disease formation in treated plants when compared to control plants. These results emphasize the importance of such studies as a tool for the development of sustainable agricultural practices that can be easily applied in our region, and also show that B. cereus and B. simplex in sunflowers can be potential bacterial bioagents that can be used in biological control against S.sclerotiorum. In addition, it will be useful to carry out studies on the development of commercial preparations of the bacterial isolates found in the study.

Keywords

Sunflower, White rot, Biological control, Sclerotinia sclerotiorum

Project Number

18101017 nolu proje

Ayçiçeğinde Sclerotinia sclerotiorum (Lib.) De Bary’ye Karşı Bazı Bakteriyel Antagonistlerin In vitro ve In vivo Etkinliklerinin Belirlenmesi

Abstract

Bu çalışma Konya ve Aksaray illeri ayçiçek ekim alanlarında kök ve kök boğazı çürüklüğüne neden olan Sclerotinia sclerotiorum’a karşı bazı bakteriyel biyoajanların in vitro ve in vivo etkinliklerini belirlemek amacıyla 2017-2018 yıllarında yürütülmüştür. Konya ve Aksaray illeri ayçiçek ekim alanlarından toplanan hastalıklı bitkilerden izole edilip, tanılaması yapıldıktan sonra patojenisite testleri sonucu seçilen en virulent olan iki S. sclerotiorum (Hırkatol ve Eskil) izolatına karşı yine aynı alanlardan sağlıklı ayçiçek bitkilerinin rizosfer bölgesindeki topraktan izole edilen 16 bakteri izolatının antifungal etkileri değerlendirilmiştir. Öncelikli olarak ikili kültür testleri ile en etkili bakteriyel biyoajanlar belirlenmiştir. In vitro testler sonucunda en geniş zon çapı oluşturan toplam 5 bakteri izolatının 16S rRNA’ya göre moleküler olarak tanılaması yapılmış ve saksı denemelerinde kullanılmıştır. Bakteriler Bacillus cereus, Bacillus simplex, Brevibacterium frigoritolerans, Bacillus toyonensis (2 izolat) olarak teşhis edilmiş ve GenBank veritabanının (NCBI) BLAST programı kullanılarak kodlanmıştır. In vitro da S. sclerotiorum’un her iki izolatında da en yüksek etki %49.19-57.95 engelleme oranıyla Bacillus cereus ve Bacillus simplex’ de gözlenmiştir. In vivo da test edilen bakteri türlerinden Bacillus toyonensis (B1) hariç bütün bakteriler kontrole göre lezyon gelişimini azaltmış veya durdurmuştur. Uygulama sonucunda biyolojik mücadele ajanı bakterilerden Bacillus cereus ve Bacillus simplex in vivo koşullarda S. sclerotiorum’un her iki izolatının da gelişmesine tamamen (%100) engel olmuştur. Yapılan etkinlik çalışmalarında bakteriyel izolatların hem ayçiçeği bitkisinin sağlıklı gelişmesine neden olduğu hem de uygulama yapılmış bitkilerde hastalık oluşumunu kontrollerdeki bitkilerle karşılaştırıldığında önemli düzeyde engellediğini göstermiştir. Bu sonuçlar bu tür çalışmaların bölgemizde kolaylıkla uygulanabilecek sürdürülebilir tarım uygulamalarının geliştirilmesi için bir araç olarak önemini vurgulamakta aynı zamanda ayçiçeğinde S.sclerotiorum’a karşı B.cereus ve B.simplex’in biyolojik mücadelede kullanılabilecek potansiyel bakteriyel biyoajanlar olabileceklerini göstermektedir. Ayrıca çalışmada etkin bulunan bakteri izolatlarının ticari preparatlarının geliştirilmesine yönelik çalışmaların yapılması yararlı olacaktır.

Keywords

Ayçiçeği, beyaz çürüklük, biyolojik mücadele, Sclerotinia sclerotiiorum

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

18101017 nolu proje

Thanks

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğüne desteklerinden dolayı teşekkür ederiz.

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