Su Kabağı ve Acur’da Doğal Enfeksiyona Neden Olan Karpuz Mozaik Virüsü (WMV)’nün İlk Raporu

Year 2024, , 206 - 218, 30.01.2024

Abdullah Güller , Mustafa Usta , Serap Demirel , Gülüstan Korkmaz

https://doi.org/10.33462/jotaf.1260007

Abstract

Tarımın ana ürünlerinden biri olan kabakgiller, birçok bitki virüsüne karşı hassastır. Ağustos 2019’da Antalya ve Denizli ilinde (Türkiye) müstakil ev bahçelerinde yetiştirilen bazı kabakgil bitkilerinde virüs tipi semptomlar gözlenmiştir. Çoğu belirtili olan bitkilerden (kavun (Cucumis melo L.), karpuz (Citrullus lanatus L.), su kabağı (Lagenaria siceraria (Molina) Standl.) ve acur (Cucumis melo var. flexuosus) toplam 53 yaprak örneği toplanmış ve olası watermelon mosaic virus (WMV) enfeksiyonuna karşı Revers-Transkriptaz Polimeraz Zincir Reaksiyonu (RT-PCR) ile test edilmiştir. Kılıf protein genine (CP) özel primer setleri, simptomatik bitkilerden yaklaşık 820 bp’lik bir gen ürününü amplifiye etmiştir. WMV enfeksiyonları, 11 kavun, 8 karpuz, 7 acur ve 5 su kabağı dahil olmak üzere 31 farklı kabakgil bitkisinde tespit edilmiştir. Antalya ilinde sadece su kabağı bitkilerinde, Denizli'den toplanan tüm kabakgil örneklerinde viral enfeksiyon varlığı saptanmıştır. Virüs izolatlarının moleküler özelliklerinin daha iyi anlaşılması için amplifiye edilmiş viral DNA fragmanları uygun bir prokaryotik plazmide klonlanmış, Yeni Nesil Dizileme (NGS) ile dizilenmiş ve gen bankasına kaydı yapılmıştır. Basic Local Alignment Search Tool (BLAST) ile gerçekleştirilen biyoinformatik analizler, belirlenen WMV-CP gen dizilerinin dünyadaki diğer izolatlarınkiyle yüksek bir nükleotid benzerlik indeksi ile desteklenerek önemli bir nükleotit homojenitesi sergilediğini göstermiştir. Ayrıca, Antalya ve Denizli illerinden izole edilen Türk izolatları kendi aralarında yaklaşık %94 oranında nükleotit benzerliği göstermiştir. Filogenetik çıkarımlar için, WMV dizileri, aynı virüslerin farklı coğrafik kökenlerinden gelen 30 izolatla çoklu hizalamaya tabi tutulmuştur. Moleküler filogeni ise tüm WMV izolatlarının değişen oranlarda diğer dünya WMV izolatları ile yakından ilişkili olduğunu göstermiştir. WMV, kabakgil bitkilerinde geniş konukçu dizisine sahip virüsler arasında yer almakta ve bununla birlikte bu çalışma, Türkiye’nin Güney ve Batı Bölgeleri’nde su kabağında ve acurda belirlenen WMV izolatlarının tüm dünyadaki ilk bilimsel raporudur.WMV, kabakgil bitkilerinde geniş spektrumlu virüslerdir. Bu çalışma, Türkiye'nin Güney-Batı Bölgesi’nden su kabağı ve acurda belirlenen Karpuz mozaik potyvirüs izolatlarının tüm dünyadaki ilk bilimsel raporudur.

Keywords

WMV, RT-PCR, Moleküler filogeni, Süs kabağı, Acur

Project Number

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First Report of Natural Infection of Watermelon mosaic virus (WMV) Infecting Bottle Gourd and Snake Melon

Abstract

Cucurbitaceous crops, one of the main crops of agriculture, are sensitive to many plant viruses. In August 2019, virus-like symptoms were observed on some cucurbit plants grown in private home gardens in Antalya and Denizli provinces (Turkey). A total of 53 leaf samples were sampled from plants with the most symptoms (melon (Cucumis melo L.), watermelon (Citrullus lanatus L.), bottle gourd (Lagenaria siceraria (Molina) Standl.), and snake melon (Cucumis melo var. flexuosus) and tested by Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) against possible watermelon mosaic potyvirus (WMV) infection. The coat protein gene (CP) specific primer sets amplified a gene product of nearly 820 bp fragment from symptomatic plants. WMV infections were detected in 31 individual cucurbit plants, including 11 melons, 8 watermelons, 7 snake melons and 5 bottle gourds. The presence of viral infection was found only in ornamental squash plants in Antalya province and in all cucurbits sampled in Denizli province. To better comprehend the molecular characteristics of virus isolates, the amplified viral DNA fragments were cloned in a proper prokaryotic plasmid, sequenced by Next Generation Sequencing (NGS) and recorded to GenBank. Bioinformatic analyses using the Basic Local Alignment Search Tool (BLAST) showed that the identified CP gene sequences exhibited significant nucleotide homogeneity, supported by a high nucleotide similarity index with that of other isolates around the world. In addition, Turkish isolates isolated from Antalya and Denizli regions showed approximately 94% nucleotide similarity among themselves. For phylogenetic inference, WMV sequences were subjected to multiple alignments with isolates from different geographic origins of the same viruses. Molecular phylogeny showed that all WMV isolates are closely related to other world WMV isolates at variable rates. WMV is wide host range viruses in cucurbit crops, however, this work is the first scientific report of WMV isolates detected in bottle gourd and snake melon from the South and West Regions of Turkey all over the world.WMV are broad spectrum viruses in cucurbit crops. This work is the first scientific report of Watermelon mosaic potyvirus isolates detected in bottle gourd and snake melon from the South-West Region of Turkey in all over the world.

Keywords

WMV, RT-PCR, Molecular Phylogeny, Bottle gourd, Snake melon

Supporting Institution

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Project Number

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Thanks

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