Effects of Iso-Osmotic Potential of NaCl and PEG6000 Solutions on Germination and Initial Seedling Growth of Sweet White Lupin (Lupinus albus L)

Abstract

Sweet white lupin has a growing importance as a staple crop due to its rich protein and mineral content. Investigation of unfavorable environmental conditions at the seedling stage is critical for understanding and overcoming the challenges during germination and initial growth. In this study, the effects of salt and drought stress were investigated upon induction of NaCl and PEG6000 osmotic agents at iso-osmotic potential (0, -0.3 and -0.6 MPa) on seed germination and initial seedling growth in sweet white lupin (Lupinus albus L.). The research was carried out for 21 days under laboratory conditions according to a completely randomized plot design with 4 replicates. In order to assess the growth upon stress, germination percentage, mean germination time, germination rate index, shoot and root length, root/shoot length, shoot and root fresh weight, shoot and root dry weight, shoot and root dry matter content, root/shoot dry matter content, shoot and root water content and seedling vigor index parameters were measured. The results demonstrated that NaCl and PEG6000 solutions applied at the same osmotic potential had statistically significant effects on the measured germination and growth parameters. PEG6000 treatments at the same osmotic potential had more adverse effects on germination and initial seedling growth than NaCl treatments. In addition, shoot growth was more adversely affected than root growth in PEG6000 and NaCl treatments. The germination was limited in -0.6 MPa PEG6000 treatment and no subsequent seedling growth was observed. In this study, we documented that the white lupine's tolerance to drought during germination and initial seedling growth periods was lower than salinity at the same iso-osmotic potential and saline and arid soils showing an osmotic water potential of -0.6 MPa (12.7 dS m-1 EC and 22% PEG6000) are inhibitory for lupin germination and growth. This study lays the ground for further physiological and molecular studies on the effects of salt and osmotic stress on white lupins.

Keywords

• Lupinus albus L.
• iso-osmotic potantial
• NaCl
• PEG6000
• germination
• initial seedling growth

Ethical Statement

There is no need to obtain permission from the ethics committee for this study.

İzo-Ozmotik NaCl ve PEG6000 Solüsyonlarının Tatlı Beyaz Acı Baklanın (Lupinus albus L.) Çimlenme ve İlk Fide Gelişimi Üzerine Etkileri

Abstract

Tatlı beyaz acı bakla, zengin protein ve mineral içeriği nedeniyle temel bir ürün olarak giderek artan bir öneme sahiptir. Fide aşamasında elverişsiz çevre koşullarının araştırılması, çimlenme ve erken büyüme sırasındaki zorlukların anlaşılması ve üstesinden gelinmesi için kritik öneme sahiptir. Bu çalışmada, tatlı beyaz acı baklada (Lupinus albus L.) tohum çimlenmesi ve ilk fide büyümesi üzerine NaCl ve PEG6000 ozmotik ajanlarının izo-ozmotik potansiyelde (0, -0,3 ve -0,6 MPa) indüksiyonu ile oluşturulan tuz ve kuraklık stresinin etkileri araştırılmıştır. Araştırma, tesadüfi parseller deneme desenine göre 4 tekerrür olacak şekilde laboratuvar koşullarında 21 gün boyunca yürütülmüştür. Stres altındaki büyümeyi değerlendirmek amacıyla çimlenme yüzdesi, ortalama çimlenme süresi, çimlenme indeksi, sürgün ve kök uzunluğu, kök/sürgün oranı, sürgün ve kök taze ağırlığı, sürgün ve kök kuru ağırlığı, sürgün ve kök kuru madde içeriği, kök/sürgün kuru madde içeriği, sürgün ve kök su içeriği ve fide canlılık indeksi parametreleri ölçülmüştür. Sonuçlar, aynı ozmotik potansiyelde uygulanan NaCl ve PEG6000 çözeltilerinin ölçülen çimlenme ve büyüme parametreleri üzerinde istatistiksel olarak anlamlı etkilere sahip olduğunu göstermiştir. Aynı ozmotik potansiyelde PEG6000 uygulamaları, NaCl uygulamalarına kıyasla çimlenme ve ilk fide büyümesi üzerinde daha olumsuz etkilere sahip olmuştur. Ayrıca, PEG6000 ve NaCl uygulamalarında sürgün büyümesi kök büyümesine kıyasla daha olumsuz etkilenmiştir. Çimlenme -0.6 MPa PEG6000 uygulamasında sınırlı kalmış ve sonuç olarak fide büyümesi gözlenmemiştir. Bu çalışmada, tatlı beyaz acı baklanın çimlenme ve ilk fide büyüme dönemlerinde kuraklığa karşı toleransının, aynı izo-ozmotik potansiyeldeki tuzluluktan daha düşük olduğunu ve -0.6 MPa (12,7 dS/m EC ve %22 PEG6000) ozmotik su potansiyeli gösteren tuzlu ve kurak toprakların acı bakla çimlenmesi ve büyümesi için engelleyici olduğunu belgelenmiştir. Bu çalışma, tuz ve ozmotik stresin beyaz acı bakla üzerindeki etkileri konusunda daha ileri fizyolojik ve moleküler çalışmalar yapılmasına zemin hazırlamaktadır.

Keywords

• Lupinus albus L.
• izo-ozmotik potansiyel
• NaCl
• PEG6000
• çimlenme
• ilk fide büyümesi

Ethical Statement

Bu çalışma için etik kuruldan izin alınmasına gerek yoktur.

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