Sultana Üzüm Çeşitlerinde (Vitis vinifera L.) in vitro Kalus Rejenerasyonu için Eksplant Materyali ve Medya Seçimi

Abstract

Bu çalışma, Sultana'da (Vitis vinifera L.) kallus rejenerasyonu için eksplant materyali ve kültür ortamının seçimi için gerçekleştirilmiştir.

Keywords

• Sultana (Vitis vinifera L.),doku kültürü,kallus,yaprak disk

Choise of Explant Material and Media for in vitro Callus Regeneration in Sultana Grape Cultivar (Vitis vinifera L.)

Abstract

The study was carried out to choise of explant material and culture media for callus regeneration in ‘Sultana’ (Vitis

vinifera L.). Leaf disc and node explants were the main explant materials which were taken from in vitro shoots which

were obtained from macroshoot tip explants. The initial macroshoot tip were cultured on MS (Murashige and Skoog)

medium including BAP (6-benzylaminopurine) (1 mg L-1) and in vitro shoots were subcultured on shoot multiplication

medium with BAP (1 mg L-1) + IBA (Indole-3-butyric acid) (0.1 mg L-1) In order to investigate callus regeneration

potential of Sultana grape cultivar, leaf disc explants were cultured on two different MS medium including BAP in

combination with 2,4-D (2,4-dichlorophenoxyacetic acid) whereas node explants cultured on four different MS

medium including BAP in combination with 2,4-D and NAA (naphthalene acetic acid). The intensity of callus

proliferation was greater in leaf disc culture than in node culture. In all media combinations, MS medium including

BAP (1 mg L-1) + 2,4-D (0.1 mg L-1) were found to be the most effective on callus regeneration. In this medium, callus

regeneration rate was found to be 100% and the average diameter of callus was found to be 6.3 mm.

Keywords

• Sultana’ (Vitis vinifera L.),tissue culture,callus,leaf disc

References

1. Acanda, Y., M.J. Prado, M.V. González, and M. Rey, 2013. Somatic embryogenesis from stamen filaments in grapevine (Vitis vinifera L. cv. Mencía): changes in ploidy level and nuclear DNA content. In Vitro Cellular &Developmental Biology-Plant, 49(3):276-284.