Determination of Germination Characteristics of Covered Alfalfa (Medicago sativa L.) Seeds in Drought Stress Conditions

Year 2020, Volume: 17 Issue: 2, 124 - 136, 27.05.2020

Mehmet Arslan , Teslime Bıçakcı Elçin Aksu

https://doi.org/10.33462/jotaf.520836

Cited By: 3

Abstract

Alfalfa (Medicago sativa L.) is an essential hay product and a protein-rich forage crop which grow in arid and semi arid regions of the world. In this study, to improve the germination properties of alfalfa seeds, which are sensitive to environmental conditions during germination and early seedling development was aimed. For this purpose, seeds of Nimet alfalfa variety were covered with seed coating preparation, named Panoramix and germinated under drought stress conditions. Panoramix was applied in two concentrations; zero (control), 2 liters 1000 kg of seeds1 and 4 liters 1000 kg of seeds-1. A laboratory germination experiment was conducted in randomized parcel design with 4 repetitions. Six different drought stress levels (0, -2, -4, -6, -8 ve -9.8 bar) were handled in this study and were used Polyethylene glycol-6000 (PEG-6000) to create of drought stress at different levels. Panoramix was applied in 3 doses, as 0 doses (control), 2 liters / 1000 kg seeds and 4 liters/1000 kg seeds respectively. 50 seeds was laid in each petri dish and placed in germination cabinet (70% humidity, 20 °C temperature 14 hours light (2000 lux) 10 hours darkness). A greenhouse emergence test was performed using plastic pots filled with a mixture 50% soil + 50% sand. Four different drought stresses [0 (T0), %25 (T1), %50 (T2), %75 (T3)] were applied to the pots. At the end of the both experiments, germination rate was calculated and the fresh and dry weight of the shoots and roots, length of shoots and roots were determined. The results indicated that the coating treatmens positive contributied to the germination properties of alfalfa seeds under drought stress conditions.

Keywords

Medicago sativa L., seed coating, drought, germination characteristics

Kaplanmış Yonca (Medicago sativa L.) Tohumlarının Kuraklık Stresi Koşullarında Çimlenme Özelliklerinin Belirlenmesi

Abstract

Yonca dünyanın ve Türkiye’nin
kurak ve yarı kurak bölgelerinde yetiştirilen, otu proteince zengin bir yem
bitkisidir. Bu çalışmada, çimlenme ve erken fide gelişimi döneminde çevresel
koşullara çok hassas olan yonca tohumlarının çimlenme özelliklerinin geliştirilmesi
amaçlanmıştır. Bu amaçla Nimet yonca çeşidinin tohumları Panoramix adlı tohum
kaplama preparatı ile kaplanmış ve kuraklık stresi koşullarında
çimlendirilmiştir. Denemede Panoramix  2 L/1000
kg tohum ve 4 L/1000 kg tohum olmak üzere 2 doz uygulanmıştır. Laboratuarda
yürütülen çalışma, tesadüf parselleri deneme desenine göre 4 tekerrürlü olarak
petri kutularında gerçekleştirilmiştir. Her petriye 50 tohum yerleştirilmiş olup;
petriler %70 nem, 20oC sıcaklık, 14 saat ışıklı ve 10 saat karanlık
olacak şekilde ayarlanmış iklimlendirme kabininde bekletilmiştir. Kuraklık
stresi ise 0, -2, -4, -6, -8 ve
-9.8 bar olacak şekilde Polyethylene glycol-6000 (PEG-6000) ile
oluşturulmuştur. Sera denemesi, 3 tekerrürlü olarak, %50 kum + %50 toprak
karışımı ile doldurulan plastik saksılarda (1.5 L) yürütülmüş ve 4 farklı
kuraklık stresi [0 (T0), %25 (T1), %50 (T2), %75 (T3)] uygulanmıştır. Denemenin sonunda,
çimlenme oranı, kökçük yaş ve kuru ağırlığı, sapçık yaş ve kuru ağırlığı
belirlenmiştir. Sonuçta, tohum kaplama uygulamalarının yonca tohumlarının
kuraklık stresi koşullarında çimlenme özelliklerine olumlu katkı yaptığı
belirlenmiştir. 

Keywords

Mecidago sativa L., tohum kaplama, kuraklık

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