Year 2020, Volume 17 , Issue 2, Pages 264 - 275 2020-05-27

Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings
Servi fidanlarında sorun olan fungal hastalık etmenlerine karşı defne ve rezene uçucu yağlarının kimyasal bileşenleri ve antifungal etkinliğinin belirlenmesi

Merve KARA [1] , Soner SOYLU [2] , Musa TÜRKMEN [3] , Alpaslan KAYA [4]


Fusarium oxysporum and Pestalotiopsis funerea are the most common fungal disease agents of conifer seedlings causing root rot and shoot or tip blight diseases. In this study, chemical compositions and antifungal activities of essential oils of fennel (Foeniculum vulgare Mill.) and laurel (Laurus nobilis L.) were determined against root rot and wilt disease agents F. oxysporum and P. funerea in vitro conditions. Chemical compositions of essential oils were determined by using GC-MS analysis. Antifungal volatile phase effects of essential oils were determined on inhibition of mycelial growth in vitro conditions by using different concentrations. The effect of most effective concentrations of essential oils on the morphology of fungal hypha was also determined by using light microscope. GC-MS analysis of essential oils of laurel and fennel plants revealed that eucalyptol (46.97%) and α-terpinyl acetate (19.82%) were major components of laurel; trans-anethole (82.44%) and limonene (5.18%) were major components of fennel essential oils. Volatile phase effects of fennel and laurel essential oils were found to completely inhibit mycelial growth of F. oxysporum at 30.0 and 50.0 µl petri-1 concentrations, respectively. Complete growth inhibition of P. funerea by essential oil of fennel and laurel were observed at relatively lower concentrations (20.0 and 25.0 µl petri-1 concentrations, respectively). Light microscopic observations on hyphae, exposed to volatile phase of the most efficient concentrations of essential oil, revealed considerable structural deformations such as cytoplasmic coagulation, vacuolations and protoplast leakage. In conclusion, our results suggest that essential oils have the potential for use in control of fungal diseases of conifer plants.
Fusarium oxysporum ve Pestalotiopsis funerea, kozalaklı bitki türlerinde kök çürüklüğü ve sürgün ya da uç yanıklığı hastalıklarına sebep olan en yaygın fungal hastalıklardır. Bu çalışmada, rezene (Foeniculum vulgare Mill.) ve defne (Laurus nobilis L.) bitkilerinden elde edilen uçucu yağların kimyasal bileşimleri ve F. oxysporum ve P. funerea’ya karşı antifungal etkileri in vitro koşullarda belirlenmiştir. Uçucu yağların kimyasal bileşimleri, GC-MS analizi kullanarak belirlenmiştir. Uçucu yağların buhar fazında misel gelişimini engellemesi üzerine antifungal etkileri in vitro koşullarda farklı konsantrasyonlar kullanarak belirlenmiştir. Işık mikroskobu kullanarak uçucu yağların en etkili konsantrasyonlarının fungal hiflerin morfolojisine etkileri de belirlenmiştir. Uçucu yağlarının GC-MS analizleri, eucalyptol (%46.97) ve α-terpinyl acetate (%19.82)’ın defnenin, trans-anethole (%82.44) ve limonene (%5.18) ise rezene uçucu yağının ana bileşenleri olduğunu göstermiştir. Rezene ve defne uçucu yağlarının buhar fazında F. oxysporum’un miselyal gelişimini tamamen engelleyen konsantrasyonları sırasıyla 30.0 ve 50.0 µl petri-1 olarak belirlenmiştir. Rezene ve defne uçucu yağlarının P. funerea’nın gelişimini tamamen engelleyen konsantrasyonların nispeten daha düşük olduğu gözlenmiştir (sırasıyla 20.0 ve 25.0 µl petri-1 konsantrasyonlarında). Uçucu yağların buhar fazında en etkili konsantrasyonuna maruz kalan hifler üzerinde yapılan ışık mikroskobu gözlemleri, sitoplazmik pıhtılaşma, vakuolleşme ve protoplazma akıntısı gibi önemli yapısal deformasyonlar ortaya çıkarmıştır. Sonuç olarak bulgularımız uçucu yağların kozalaklı bitkilerin fungal hastalıklarının kontrolünde kullanım potansiyeli bulunduğunu ortaya koymaktadır.
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Primary Language en
Subjects Science
Journal Section Articles
Authors

Orcid: 0000-0001-7320-3376
Author: Merve KARA
Institution: MUSTAFA KEMAL UNIVERSITY
Country: Turkey


Orcid: 0000-0003-1002-8958
Author: Soner SOYLU (Primary Author)
Institution: Mustafa Kemal Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü
Country: Turkey


Orcid: 0000-0001-9914-9523
Author: Musa TÜRKMEN
Institution: MUSTAFA KEMAL UNIVERSITY
Country: Turkey


Orcid: 0000-0003-3544-9214
Author: Alpaslan KAYA
Institution: MUSTAFA KEMAL UNIVERSITY
Country: Turkey


Thanks Bu çalışma "3rd Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP-3) Merit Park Hotel in Girne (Kyrenia), Turkish Republic of Northern Cyprus (TRNC) April 13-16, 2017" bildiri kitapçığında ÖZET olarak sunulmuştur
Dates

Application Date : December 23, 2019
Acceptance Date : May 7, 2020
Publication Date : May 27, 2020

Bibtex @research article { jotaf663452, journal = {Tekirdağ Ziraat Fakültesi Dergisi}, issn = {1302-7050}, eissn = {2146-5894}, address = {}, publisher = {Namik Kemal University}, year = {2020}, volume = {17}, pages = {264 - 275}, doi = {10.33462/jotaf.663452}, title = {Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings}, key = {cite}, author = {Kara, Merve and Soylu, Soner and Türkmen, Musa and Kaya, Alpaslan} }
APA Kara, M , Soylu, S , Türkmen, M , Kaya, A . (2020). Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings . Tekirdağ Ziraat Fakültesi Dergisi , 17 (2) , 264-275 . DOI: 10.33462/jotaf.663452
MLA Kara, M , Soylu, S , Türkmen, M , Kaya, A . "Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings" . Tekirdağ Ziraat Fakültesi Dergisi 17 (2020 ): 264-275 <https://dergipark.org.tr/en/pub/jotaf/issue/54544/663452>
Chicago Kara, M , Soylu, S , Türkmen, M , Kaya, A . "Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings". Tekirdağ Ziraat Fakültesi Dergisi 17 (2020 ): 264-275
RIS TY - JOUR T1 - Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings AU - Merve Kara , Soner Soylu , Musa Türkmen , Alpaslan Kaya Y1 - 2020 PY - 2020 N1 - doi: 10.33462/jotaf.663452 DO - 10.33462/jotaf.663452 T2 - Tekirdağ Ziraat Fakültesi Dergisi JF - Journal JO - JOR SP - 264 EP - 275 VL - 17 IS - 2 SN - 1302-7050-2146-5894 M3 - doi: 10.33462/jotaf.663452 UR - https://doi.org/10.33462/jotaf.663452 Y2 - 2020 ER -
EndNote %0 Tekirdağ Ziraat Fakültesi Dergisi Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings %A Merve Kara , Soner Soylu , Musa Türkmen , Alpaslan Kaya %T Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings %D 2020 %J Tekirdağ Ziraat Fakültesi Dergisi %P 1302-7050-2146-5894 %V 17 %N 2 %R doi: 10.33462/jotaf.663452 %U 10.33462/jotaf.663452
ISNAD Kara, Merve , Soylu, Soner , Türkmen, Musa , Kaya, Alpaslan . "Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings". Tekirdağ Ziraat Fakültesi Dergisi 17 / 2 (May 2020): 264-275 . https://doi.org/10.33462/jotaf.663452
AMA Kara M , Soylu S , Türkmen M , Kaya A . Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings. Tekirdağ Ziraat Fakültesi Dergisi. 2020; 17(2): 264-275.
Vancouver Kara M , Soylu S , Türkmen M , Kaya A . Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings. Tekirdağ Ziraat Fakültesi Dergisi. 2020; 17(2): 264-275.