Evaluation of the Genetic Structure of Some Accessions Belonging to Onobrychis spp. Using Microsatellite DNA Markers

Year 2023, Volume: 20 Issue: 2, 399 - 409, 22.05.2023

Elbi Cansu Yılmaz , Behiye Banu Bilgen , Metin Tuna

https://doi.org/10.33462/jotaf.1141367

Cited By: 1

Abstract

Turkey is in a very convenient position for animal husbandry in terms of both natural resources and ecological conditions. Forage crops, which has a very important place in agricultural activities, is the insurance of plant and animal production. Sainfoin is a perennial forage legume species that grown in the northern temperate regions of the world from the Mediterranean region and the Caucasus, and to Central Asia. In this study the genetic diversity of 100 genotypes representing 44 accessions from 18 different Onobrychis species (O. arenaria subsp. arenaria, O. inermis, O. petraea, O. cyri, O. iberica, O. altissima, O. vassilczenkoi, O. conferta subsp. argentea, O. alba subsp. laconica, O. biebersteinii, O. grandis, O. kachetica, O. kemulariae, O. oxyodonta, O. megataphros, O. pallasii, Onobrychis spp., and O. viciifolia) were evaluated using 8 simple sequence repeat (microsatellite) markers. Based on the results, OVK036, OVK094, OVK125, OVM033, OVK161, OVK046, OVM061, and OVK174 loci were polymorphic. The observed number of alleles per SSR locus ranged from 6 to 21 alleles (mean of 11.625). Maximum allele frequency ranged from 0.51 to 0.93 with a mean value of 0.73. The PIC value ranged from 0.124 to 0.244. The mean polymorphism information content of loci was 0.188. Genetic diversity coefficients according to the UPGMA ranged from 0.000 to 0.9375. Cluster analysis divided the 100 sainfoin genotypes into two main groups (Cluster-I and Cluster-II). All diploid genotypes (except for 1 diploid genotype) used in the study formed a separate group within Cluster-I. The results revealed that SSR markers used in this study are useful for molecular characterization and assessing genetic diversity of sainfoin accessions. The obtained SSR alleles and genetic variability in a studied certain loci provided significant information about the genetic structure of sainfoin accessions that could be used as parental lines in sainfoin breeding programs.

Keywords

Breeding, Molecular Characterization, Sainfoin, SSR, UPGMA

Supporting Institution

The Scientific and Technological Research Council of Turkey (TÜBİTAK)

Project Number

215O526

Thanks

This work was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK, Project No: 215O526).

Evaluation of the Genetic Structure of Some Accessions Belonging to Onobrychis spp. Using Microsatellite DNA Markers

Abstract

Türkiye hem doğal kaynaklar hem de ekolojik koşullar bakımından hayvancılığa oldukça elverişli bir konuma sahiptir. Tarımsal faaliyetler içerisinde çok önemli bir yere sahip olan yem bitkileri tarımı, bitkisel ve hayvansal üretimin sigortası konumundadır. Korunga, dünyanın kuzey ılıman bölgelerinde özellikle Akdeniz bölgesi ve Kafkaslardan Orta Asya'ya kadar yetişen çok yıllık bir baklagil yem bitkisi türüdür. Bu çalışmada 18 farklı Onobrychis türüne ait 44 aksesyonu temsil eden 100 genotipin (O. arenaria subsp. arenaria, O. inermis, O. petraea, O. cyri, O. iberica, O. altissima, O. vassilczenkoi, O. conferta subsp. argentea, O. alba subsp. laconica, O. biebersteinii, O. grandis, O. kachetica, O. kemulariae, O. oxyodonta, O. megataphros, O. pallasii, Onobrychis spp. ve O. viciifolia) genetik çeşitliliği 8 adet basit dizi tekrarları (mikrosatellit) belirteci kullanılarak incelenmiştir. Mikrosatellit analizi sonuçlarına göre, OVK036, OVK094, OVK125, OVM033, OVK161, OVK046, OVM061 ve OVK174 lokusları polimorfik olarak saptanmıştır. SSR lokusu başına gözlenen allel sayısı 6 ile 21 arasında gözlenmiştir (ortalama 11.625). Maksimum allel frekansı 0.51 ile 0.93 arasında değişmekte olup, ortalama 0.73 değerindedir. PIC değeri 0.124 ile 0.244 arasında hesaplanmış, ortalama polimorfik bilgi içeriği 0.188 olarak belirlenmiştir. UPGMA'ya göre genetik çeşitlilik katsayıları 0.000 ile 0.9375 arasında değişmiştir. Dendrogram analizine göre, 100 korunga genotipi iki ana gruba (Grup-I ve Grup-II) ayrılmıştır. Çalışmada kullanılan tüm diploid genotipler (1 diploid genotip hariç) Grup-I içerisinde ayrı bir grup oluşturmuştur. Bu çalışmada kullanılan SSR belirteçleri, korunga aksesyonlarının moleküler karakterizasyonu ve genetik çeşitliliğinin değerlendirilmesi için yararlı olduğu sonucuna varılmıştır. Spesifik lokuslardan elde edilen SSR alelleri ve genetik varyasyon, korunga ıslah programlarında ebeveyn hatları olarak kullanılabilecek korunga aksesyonlarının genetik yapısı hakkında önemli bilgiler sağlamıştır.

Keywords

Islah, Moleküler karakterizasyon, Korunga, SSR, UPGMA

Project Number

215O526

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