Relationship Between Heterosis and Genetic Distance Determined by SSR Markers in Oriental Tobacco

Year 2024, Volume: 21 Issue: 4, 865 - 879, 20.09.2024

Ahmet Kınay , İbrahim Saygılı , Nejdet Kandemir

https://doi.org/10.33462/jotaf.1278798

Abstract

Hybrid cultivars could be beneficial to achieve high leaf yields while maintaining good quality properties in oriental tobacco. Identification of parents constitutes a major part of a hybrid breeding program. The aim of the present study was to determine the relationship between the genetic distance of parents determined by simple sequence repeats (SSR) markers and heterosis levels in hybrids produced from those parents for leaf yield and quality properties in oriental tobacco. Twenty-one hybrids produced by half diallel crossing among seven tobacco genotypes used in oriental tobacco production in Turkey were grown along with their parents in three locations in 2012 and 2013. Twenty-nine SSR markers were used to determine genetic distances among seven tobacco genotypes. A total of 80 alleles were produced by all twenty-nine markers. Average number of observed alleles per polymorphic marker was 2.96. Twenty-seven of 80 alleles were observed in only one of the seven parents. The polymorphic information content of markers varied from 0.215 to 0.810 (average 0.480). Mid-parent heterosis levels ranged from 18.03 to 42.00% for leaf number, between -19.75 and 38.06% for leaf width, between -17.51 and 36.25% for leaf length, between -34.38 and 76.12% for leaf yield, between -78.30 and 154.01% for sugar content and between -45.40 and 143.29% for nicotine content. Heterosis levels were correlated with genetic distances between parents for leaf number in Erbaa 2012 and Tokat 2012 locations, for leaf width in Erbaa 2013 location, for leaf length in Erbaa 2013 location, and for leaf yield in Tokat 2012 location only. SSR markers were very effective to determine genetic distance of oriental tobacco, and only two markers could distinguish all seven genotypes used in the study. The findings indicated that genetic distance determined by SSR markers used in the present study is not sufficient to predict hybrid performance in oriental tobacco.

Keywords

Nicotiana tabacum L., Genetic diversity, Hybrid, Microsatellite markers, Yield

Oryantal Tütünde SSR Markörleriyle Belirlenen Heterosis ile Genetik Mesafe Arasındaki İlişki

Abstract

Hibrit çeşitler, oryantal tütünde iyi kalite özelliklerini korurken yüksek yaprak verimi elde etmek için faydalı olabilir. Hibrit çeşitleri oluşturacak ebeveynlerin belirlenmesi hibrit çeşit ıslah programlarında önemli bir yer tutmaktadır. Bu çalışmanın amacı, basit dizi tekrarları (simple sequence repeats, SSR) markörleri ile belirlenen ebeveynlerin genetik uzaklığı ile oryantal tütünde yaprak verimi ve kalite özellikleri için bu ebeveynlerden üretilen hibritlerdeki heterozis seviyeleri arasındaki ilişkiyi belirlemektir. Türkiye’de yoğun kullanılan yedi tütün genotipinden ve bu ebeveynlerden yarım diallel melezlemeyle ile elde edilen 21 hibrit ebeveynleriyle ile üç bölgede 2012 ve 2013 yıllarında yetiştirilmiştir. Yedi tütün genotipi arasındaki genetik uzaklıklarını belirlemek için yirmi dokuz SSR markörü kullanılmıştır. Yirmi dokuz markör toplamda 80 allel üretmiştir. Polimorfik markör başına ortalama gözlenen allel sayısı 2.96 olarak belirlenmiştir. Seksen allelin 27’si sadece bir ebeveynde gözlemlenmiştir. Markörlerin polimorfik bilgi içeriği 0.215 - 0.810 (ortalama 0.480) arasında değişmiştir. Ebeveyn ortalamasına göre hesaplanan heterozis değerleri yaprak sayısı için %18.03 - %42.00, yaprak eni için %-19.75 - %38.06, yaprak boyu için %-17.51 - %36.25, yaprak verimi için %-34.38 - %76.12, şeker oranı için %78.30 - 154.01 için ve nikotin içeriği için %-45.40 - %143.29 arasında değişmiştir. Genetik uzaklık ile heterozis arasındaki korelasyon, yaprak sayısında Erbaa 2012 ve Tokat 2012, yaprak eninde Erbaa 2013, yaprak boyunda Erbaa 2013 ve yaprak veriminde Tokat 2012 lokasyonlarında önemli bulunurken, diğer lokasyonlarda önemli bulunmamıştır. Çalışmada kullanılan yedi genotipi yalnızca iki markörün ayırt edebilmesinden dolayı, SSR markörlerinin oryantal tütünün genetik uzaklıkları belirlemek için çok etkili olduğu söylenebilir. Bu araştırmada kullanılan SSR markörleriyle belirlenen genetik uzaklık değeri doğrudan hibrit performansı tahmin etmek için yeterli olmamıştır.

Keywords

Nicotiana tabacum L., Genetik çeşitlilik, Hibrit, Microsatellite markör, Verim

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