Antioxidant Activity of Bio-extract which Obtained from Hazelnut Shells and Green Leafy Covers

Abstract

The article presents the findings of an antioxidant activity assay conducted using 70% ethanol and deionized water on extracts from green leafy covering (GLC) and hazelnut hard shells (HS) that are grown in the northwest of Azerbaijan. The kinetics of each extract were examined using a UV-2700 vis spectrophotometer, and the DPPH (2,2-Diphenyl-1-picrylhydrazyl) technique was used to determine the extract's free radical scavenger activity. The results show that the bio-extracts obtained in 70% alcohol have radical scavenging activities of FRSA(hs)=59.24% and FRSA(glc)=35.72%, while the bio-extracts obtained in water have radical scavenging activities of FRSA (hs)=31.15% and FRSA(glc)=22.23%. Waste is significant for treatment, affordable, and an effective preventive measure since it is derived from raw resources.

Keywords

• hazelnut hard shell and green leafy cover
• bio-extract
• free radical scavenger activity

References

1. 1. Tyug TS, Prasad KN, Ismail A. Antioxidant capacity, phenolics and isoflavones in soybean by-products. Food Chem [Internet]. 2010 Dec 1;123(3):583–9. Available from: .
2. 2. Muraveva DA. Farmakoqnoziya. 1981. 656 p.
3. 3. Lovkova My, Rabinovich A, Ponomareva S, Buzuk G, Sokolova S. Why plants treat. Moscow: Nauka; 1990. 290 p.
4. 4. Miralieva S, Kubalova L. Биологическая роль хрома. Cовременная научная технология. 2014;7(2):90–1.
5. 5. Raxmanin O, Eqorova N, Krasovskiy Q, Mixaylova R, Алексеева А. Кремний, его биологическое действие при энтеральном поступлении в организм и гигиеническое нормирование в питьевой воде. Обзор литературы. 2017;96(5):492–8. Available from: .
6. 6. Stévigny C, Rolle L, Valentini N, Zeppa G. Optimization of extraction of phenolic content from hazelnut shell using response surface methodology. J Sci Food Agric [Internet]. 2007 Dec 24;87(15):2817–22. Available from: .
7. 7. Contini M, Baccelloni S, Massantini R, Anelli G. Extraction of natural antioxidants from hazelnut (Corylus avellana L.) shell and skin wastes by long maceration at room temperature. Food Chem [Internet]. 2008 Oct 1;110(3):659–69. Available from: .
8. 8. Oğuzkan S, Uğraș S, Can M, Uzun A, Ülger S, Üzmez Ș, et al. Biological activity analysis of hazelnut (Corylus avellana L.) green shell and leaf extracts. Kahramanmaraș Sütçü İmam Üniversitesi Doğa Bilim Derg [Internet]. 2016;19(4):373–8. Available from: .

9. 9. Bayil Oguzkan S, Karadeniz S, Karagul B, Uzun A, Aksoy ES, Guler OO, et al. Effects of some adsorbents on the pre-purification of taxol (anticancer drug) from hazelnut nutshells. Int J Pharmacol [Internet]. 2018 Aug 1;14(6):835–40. Available from: .
10. 10. Thi Hanh Phuc D, Popovich Private Bag DG, Popovich DG. Screening for paclitaxel and other taxanes in kernel and shell of Corylus avellana (Hazelnut). J Pharmacogn Phytochem [Internet]. 2017;6(2):247–54. Available from: .
11. 11. Shahidi F, Alasalvar C, Liyana-Pathirana CM. Antioxidant phytochemicals in hazelnut kernel (Corylus avellana L.) and hazelnut byproducts. J Agric Food Chem [Internet]. 2007 Feb 1;55(4):1212–20. Available from: .
12. 12. Bottone A, Cerulli A, DʼUrso G, Masullo M, Montoro P, Napolitano A, et al. Plant specialized metabolites in hazelnut (Corylus avellana) kernel and byproducts: An update on chemistry, biological activity, and analytical aspects. Planta Med [Internet]. 2019 Aug 27;85(11/12):840–55. Available from: .
13. 13. Rusu ME, Fizeșan I, Pop A, Gheldiu AM, Mocan A, Crișan G, et al. Enhanced recovery of antioxidant compounds from hazelnut (Corylus avellana L.) involucre based on extraction optimization: Phytochemical profile and biological activities. Antioxidants [Internet]. 2019 Oct 8;8(10):460. Available from: .
14. 14. Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review. J Funct Foods [Internet]. 2015 Oct 1;18:820–97. Available from: .
15. 15. Azizov F, Khalilov Z, Atayeva V, Mustafayev N, Imanlı H. Chemical composition and biological active substances from hazelnut green leafy covers. J Turkish Chem Soc Sect A Chem [Internet]. 2022 Nov 30;9(4):999–1006. Available from: .
16. 16. Ermakov A, Arasimovich V, Iarosh N, Peruanskii I, Lukovnikova G, Ikonnikova M. Metody biokhimicheskogo issledovaniia rastenii. 1987. 430 p.
17. 17. Atayeva V, Aslanov R. EPR-based study to monitor free radicals in treated silk fibroin with anthocyanins. J Turkish Chem Soc Sect A Chem [Internet]. 2022 Nov 30;9(4):1055–62. Available from: .
18. 18. Siriwardhana SSKW, Shahidi F. Antiradical activity of extracts of almond and its by‐products. J Am Oil Chem Soc [Internet]. 2002 Sep;79(9):903–8. Available from: .
19. 19. Esposito T, Sansone F, Franceschelli S, Del Gaudio P, Picerno P, Aquino R, et al. Hazelnut (Corylus avellana L.) shells extract: Phenolic composition, antioxidant effect and cytotoxic activity on human cancer cell lines. Int J Mol Sci [Internet]. 2017 Feb 13;18(2):392. Available from: .
20. 20. Del Rio D, Calani L, Dall’Asta M, Brighenti F. Polyphenolic composition of hazelnut skin. J Agric Food Chem [Internet]. 2011 Sep 28;59(18):9935–41. Available from: .
21. 21. Fraga CG. Plant polyphenols: How to translate their in vitro antioxidant actions to in vivo conditions. IUBMB Life [Internet]. 2007 Jan 3;59(4–5):308–15. Available from: .
22. 22. Vingrys K, Mathai M, Ashton JF, Stojanovska L, Vasiljevic T, McAinch AJ, et al. The effect of malting on phenolic compounds and radical scavenging activity in grains and breakfast cereals. J Food Sci [Internet]. 2022 Sep 23;87(9):4188–202. Available from: .