Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne

Sema Arısoy; Meryem Koçaş; Nevin Tuzcu; Dilara Örgül; Tansel Çomoğlu

doi:10.18596/jotcsa.1822830

Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne

Abstract

: Acne vulgaris represents a chronic inflammation of the pilosebaceous apparatus, predominantly affecting the face and upper trunk due to the high concentration of sebaceous glands in these regions. Sulfanilamide is a sulfonamide antibacterial drug, a class of antibiotics that inhibit bacterial DNA synthesis by competitive antagonism of PABA. In this study, a novel patch formulation was developed for the effective treatment of acne using biocompatible polymers, hydroxypropyl methylcellulose (HPMC) and chitosan, as the matrix materials, and sulfanilamide as the active agent. The patches were prepared employing varying concentrations of chitosan (0.25%, 0.5%, and 1%), HPMC (0.125%, 0.25%, and 0.5%), and sulfanilamide (0.25%, 0.5%, and 0.75%) solutions. In this study, customized Design of Experiments (DoE) and response surface methodology (RSM) were applied to clarify the effect of the concentration of chitosan (X₁), HPMC (X₂), and sulfanilamide (X₃) on swelling (Y₁), porosity (Y₂), and absorbency (Y₃). Multi-response optimization analysis was conducted to identify the optimal formulation exhibiting maximum absorbency, minimum swelling, and appropriate porosity. The optimized patch formulation was subsequently characterized using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), in vitro drug release studies, and antibacterial activity studies. The findings revealed that the optimized formulation achieved controlled drug release along with notable antibacterial efficacy. Therefore, a controlled-release transdermal patch was successfully formulated for the effective treatment of acne.

Keywords

Project Number

No funding received for this study

References

1. Itoh T, Tsuchida A, Muramatsu Y, Ninomiya M, Ando M, Tsukamasa Y, et al. Antimicrobial and anti-inflammatory properties of nostocionone isolated from Nostoc commune Vauch and its derivatives against Propionibacterium acnes. Anaerobe. 2014 Jun;27:56–63. doi:
2. Oztürk A. Akne vulgaris’ in Psikiyatrik Etkileri [The psychiatric impact of acne vulgaris]. Kocatepe Med J. 2014;15(3):360–4.
3. Yamaner NJ. Akne vulgarisin topikal tedavisinde Tretinoin, Eritromisin ve Tretinoin+ Eritromisinin etkinliği. [Effectiveness of tretinoin, erythromycin and tretinoin + erythromycin in the topical treatment of acne vulgaris]. 2008.
4. Zaenglein AL. Acne Vulgaris. Solomon CG, editor. N Engl J Med. 2018 Oct 4;379(14):1343–52. doi:
5. Gungor E. Acne. Turk J Dermatol. 2012 Dec 1;6(4):138–49. doi:
6. Akhavan A, Bershad S. Topical Acne Drugs: Review of Clinical Properties, Systemic Exposure, and Safety. American Journal of Clinical Dermatology. 2003;4(7):473–92. doi:
7. Combes FC. SULFANILAMIDE AND ALLIED COMPOUNDS: THEIR VALUE AND LIMITATIONS IN DERMATOLOGY. Arch Derm Syphilol. 1941 Aug 1;44(2):236. doi:
8. Aranaz I, Acosta N, Civera C, Elorza B, Mingo J, Castro C, et al. Cosmetics and Cosmeceutical Applications of Chitin, Chitosan and Their Derivatives. Polymers. 2018 Feb 22;10(2):213. doi:

9. Parhi R, Sahoo SK, Das A. Applications of polysaccharides in topical and transdermal drug delivery: A recent update of literature. Braz J Pharm Sci. 2022;58:e20802. doi:
10. Ghosh M, Singh D, Singh A. Recent advancements in nanocarrier based therapy against acne: The role of biosurfactants and status of patents. Health Sciences Review. 2023 Jun;7:100088. doi:
11. Arpa MD, Seçen İM, Erim ÜC, Hoş A, Üstündağ Okur N. Azelaic acid loaded chitosan and HPMC based hydrogels for treatment of acne: formulation, characterization, in vitro - ex vivo evaluation. Pharmaceutical Development and Technology. 2022 Mar 16;27(3):268–81. doi:
12. Goos P, Jones B. Optimal design of experiments: a case study approach. Online-Ausg. Hoboken, N.J: Wiley; 2011. 287 p.
13. Romano L, Ceccucci V, Tensi L, Santi C, Sancineto L. A Tale of Two Designs: Comparing Design of Experiment Strategies for Reaction Optimization. Chemistry Methods. 2025 Nov;5(11):e202500008. doi:
14. Arısoy S, Şalva E. Preparation and in vitro characterization of curcumin loaded Chitosan-Hyaluronic acid polyelectrolyte complex based hydrogels. Drug Development and Industrial Pharmacy. 2023 Oct 3;49(10):637–47. doi:
15. Lamparelli EP, Casagranda V, Pressato D, Maffulli N, Della Porta G, Bellini D. Synthesis and Characterization of a Novel Composite Scaffold Based on Hyaluronic Acid and Equine Type I Collagen. Pharmaceutics. 2022 Aug 23;14(9):1752. doi:
16. Han J, Zhou Z, Yin R, Yang D, Nie J. Alginate–chitosan/hydroxyapatite polyelectrolyte complex porous scaffolds: Preparation and characterization. International Journal of Biological Macromolecules. 2010 Mar;46(2):199–205. doi:
17. Suksaeree J, Monton C, Madaka F, Chusut T, Saingam W, Pichayakorn W, et al. Formulation, physicochemical characterization, and in vitro study of chitosan/HPMC blends-based herbal blended patches. AAPS PharmSciTech. 2015 Feb;16(1):171–81. doi: PubMed PMID: 25233803; PubMed Central PMCID: PMC4309821.
18. Zaidan M, Noor Rain A, Badrul A, Adlin A, Norazah A, Zakiah I, et al. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop biomed. 2005;22(2):165–70.
19. Qothrunnadaa T, Hasanah AN. Patches for acne treatment: an update on the formulation and stability test. International Journal of Applied Pharmaceutics. 2021;13(4):21–6.
20. Carotenuto F, Fiaschini N, Di Nardo P, Rinaldi A. Towards a Material-by-Design Approach to Electrospun Scaffolds for Tissue Engineering Based on Statistical Design of Experiments (DOE). Materials. 2023 Feb 12;16(4):1539. doi:
21. Jaber SA, Saadh M, Obeid MA. The effect of polymeric films of hydroxypropyl methylcellulose ( HPMC) /chitosan on ofloxacin release, diffusion, and biological activity. Polymer Engineering & Sci. 2023 Sep;63(9):2871–7. doi:
22. Tahir D, Ardiansyah A, Heryanto H, Noor EEM, Mohamed MA. Chitosan-based hydrogels: A comprehensive review of transdermal drug delivery. International Journal of Biological Macromolecules. 2025 Apr;298:140010. doi:
23. Lin X, Zhang X, Wang Y, Chen W, Zhu Z, Wang S. Hydrogels and hydrogel-based drug delivery systems for promoting refractory wound healing: Applications and prospects. International Journal of Biological Macromolecules. 2025 Jan;285:138098. doi:
24. Raju L, A.R. SCL, Udaya Prakash NK, Rajkumar E. Chitosan-terephthaldehyde hydrogels – Effect of concentration of cross-linker on structural, swelling, thermal and antimicrobial properties. Materialia. 2021 May;16:101082. doi:
25. J. ME, Solanki R, Dhanka M, Thareja P, Bhatia D. Self-healing, injectable chitosan-based hydrogels: structure, properties and biological applications. Mater Adv. 2024;5(13):5365–93. doi:
26. Dai T, Tanaka M, Huang YY, Hamblin MR. Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects. Expert Review of Anti-infective Therapy. 2011 Jul;9(7):857–79. doi: 27. Ramadhani UKS. Formulation of Acne Patch from Garcinia mangostana L Peel Extract With a Combination of Chitosan Polymer and HPMC Against Propionibacterium acnes Bacteria. MFI. 2024 Apr 6;19(1):32–41. doi:
28. Zanchetta FC, De Wever P, Morari J, Gaspar RC, Prado TPD, De Maeseneer T, et al. In Vitro and In Vivo Evaluation of Chitosan/HPMC/Insulin Hydrogel for Wound Healing Applications. Bioengineering. 2024 Feb 9;11(2):168. doi:
29. Ganesh M, Aziz AS, Ubaidulla U, Hemalatha P, Saravanakumar A, Ravikumar R, et al. Sulfanilamide and silver nanoparticles-loaded polyvinyl alcohol-chitosan composite electrospun nanofibers: Synthesis and evaluation on synergism in wound healing. Journal of Industrial and Engineering Chemistry. 2016 Jul;39:127–35. doi:
30. Jaber SA. Transdermal patches based on chitosan/hydroxypropyl methylcellulose and polyvinylpyrrolidone/hydroxypropyl methylcellulose polymer blends for gentamycin administration. Journal of Advanced Pharmaceutical Technology & Research. 2023 Jul;14(3):202–7. doi:
31. Andrade Del Olmo J, Pérez-Álvarez L, Sáez-Martínez V, Benito-Cid S, Ruiz-Rubio L, Pérez-González R, et al. Wound healing and antibacterial chitosan-genipin hydrogels with controlled drug delivery for synergistic anti-inflammatory activity. International Journal of Biological Macromolecules. 2022 Apr;203:679–94. doi:

Details

Primary Language

English

Subjects

Medicinal and Biomolecular Chemistry (Other)

Journal Section

Research Article

Authors

Sema Arısoy ^*
0000-0003-2798-1884
Türkiye

Meryem Koçaş
0000-0002-4165-6191
Türkiye

Nevin Tuzcu
0000-0001-5899-3955
Türkiye

Dilara Örgül
0000-0002-8947-4995
Türkiye

Tansel Çomoğlu
0000-0002-4221-5814
Türkiye

Publication Date

April 28, 2026

Submission Date

November 14, 2025

Acceptance Date

February 26, 2026

Published in Issue

Year 2026 Number: 2026-1

DOI

https://doi.org/10.18596/jotcsa.1822830

IZ

https://izlik.org/JA93AD82GH

Cite

RIS / Bibtex

APA

Arısoy, S., Koçaş, M., Tuzcu, N., Örgül, D., & Çomoğlu, T. (2026). Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne. Journal of the Turkish Chemical Society Section A: Chemistry, 2026-1, 1-12. https://doi.org/10.18596/jotcsa.1822830

AMA

1.Arısoy S, Koçaş M, Tuzcu N, Örgül D, Çomoğlu T. Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne. JOTCSA. 2026;(2026-1):1-12. doi:10.18596/jotcsa.1822830

Chicago

Arısoy, Sema, Meryem Koçaş, Nevin Tuzcu, Dilara Örgül, and Tansel Çomoğlu. 2026. “Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne”. Journal of the Turkish Chemical Society Section A: Chemistry, no. 2026-1: 1-12. https://doi.org/10.18596/jotcsa.1822830.

EndNote

Arısoy S, Koçaş M, Tuzcu N, Örgül D, Çomoğlu T (April 1, 2026) Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne. Journal of the Turkish Chemical Society Section A: Chemistry 2026-1 1–12.

IEEE

[1]S. Arısoy, M. Koçaş, N. Tuzcu, D. Örgül, and T. Çomoğlu, “Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne”, JOTCSA, no. 2026-1, pp. 1–12, Apr. 2026, doi: 10.18596/jotcsa.1822830.

ISNAD

Arısoy, Sema - Koçaş, Meryem - Tuzcu, Nevin - Örgül, Dilara - Çomoğlu, Tansel. “Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne”. Journal of the Turkish Chemical Society Section A: Chemistry. 2026-1 (April 1, 2026): 1-12. https://doi.org/10.18596/jotcsa.1822830.

JAMA

1.Arısoy S, Koçaş M, Tuzcu N, Örgül D, Çomoğlu T. Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne. JOTCSA. 2026;:1–12.

MLA

Arısoy, Sema, et al. “Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne”. Journal of the Turkish Chemical Society Section A: Chemistry, no. 2026-1, Apr. 2026, pp. 1-12, doi:10.18596/jotcsa.1822830.

Vancouver

1.Sema Arısoy, Meryem Koçaş, Nevin Tuzcu, Dilara Örgül, Tansel Çomoğlu. Preparation and Characterization of Sulfanilamide-Loaded Patches for the Treatment of Acne. JOTCSA. 2026 Apr. 1;(2026-1):1-12. doi:10.18596/jotcsa.1822830