Research Article

Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives

Volume: 2026 Number: 2 November 8, 2026

Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives

Abstract

In this work, a series of new benzodioxolyl pyrazoline derivatives (a6-10) has been synthesized successfully. Some of the different aromatic aldehydes have been added to benzodioxolyl keto compound in strong alkaline media to produce chalcones (a1-5). These chalcones were been converted to pyrazoline through cyclization reaction using phenyl hydrazine as cyclization agent. The structures of the prepared compounds have been proved in terms of the structure characterization depending on the spectral data, using spectroscopy techniques such as Fourier Transform Infrared (FTIR), Proton Nuclear Magnetic Resonance (1HNMR) and Carbon Nuclear Magnetic Resonance 13CNMR. In addition to using of Gas Chromatography Mass Spectroscopy (GC mass) for the final series of the new pyrazole derivatives to verify their structures. These synthesized compounds have been screened for their antibacterial activity effect evaluation towards Staphylococcus aureus and Staphylococcus epidermidis (Gram-positive bacteria) and Escherichia coli and Pseudomonas aeruginosa (Gram-negative bacteria). Additionally, the Free Radical Scavenging Activity (RSA) of the synthesized pyrazoline (a6-10) has been assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) as antioxidant. The results indicated lack of the antibacterial and antioxidant activity effect. For the antibacterial activity effect results, it could be related to penetrate of bacterial cellular targets insufficiently and the absence of active centers in pyrazoline structure for binding to bacteria’s outer cell sufficiently. In the case of antioxidant test, one of the reasons is could be the absence of some good electron-releasing substituents such hydroxy (OH), amine (NH) and thiol (SH) having the ability to donate hydrogen atoms or electrons efficiently to neutralize free radicals. These pyrazoline derivatives don’t behave as active biomaterial characteristics against the tested bacteria for both antibacterial and antioxidant activity effect.

Keywords

Thanks

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References

  1. 1. El Hassani IA, Rouzi K, Assila H, Karrouchi K, Ansar M. Recent advances in the synthesis of pyrazole derivatives: A review. Reactions. 2023 Sep 5;4(3):478–504. Available from: .
  2. 2. Becerra D, Abonia R, Castillo JC. Recent applications of the multicomponent synthesis for bioactive pyrazole derivatives. Molecules. 2022 Jul 23;27(15):4723. Available from: .
  3. 3. Verma A, Sharma S, Chauhan R. Recent chemical advancements of pyrazole moiety in anticancer therapy. Int J Eng Sci Res Technol. 2016;5(4):869–76. Available from: .
  4. 4. Mukhtar S, Morsy N, Hassan A, Hafez T, Hassaneen H, Saleh F. A Review of Chalcones: Synthesis, Reactions, and Biological Importance. Egypt J Chem. 2022 Jan 15;65(8):379–95. Available from: .
  5. 5. Bennani FE, Doudach L, Cherrah Y, Ramli Y, Karrouchi K, Ansar M, et al. Overview of recent developments of pyrazole derivatives as an anticancer agent in different cell line. Bioorg Chem. 2020 Apr;97:103470. Available from: .
  6. 6. Rani P, Dhillon S, Kumari G, Chahal M, Aneja DK, Pareek B, et al. Advances in the synthesis and biological functionality of pyrazolines and pyrazoles from chalcone precursors. Bull Korean Chem Soc. 2025 May 13;46(5):481–515. Available from: .
  7. 7. El-Naggar M, Rashdan HRM, Abdelmonsef AH. Cyclization of chalcone derivatives: Design, synthesis, In Silico docking study, and biological evaluation of new quinazolin-2,4-diones incorporating five-, six-, and seven-membered ring moieties as potent antibacterial inhibitors. ACS Omega. 2023 Aug 1;8(30):27216–30. Available from: .
  8. 8. Elkanzi NAA, Hrichi H, Alolayan RA, Derafa W, Zahou FM, Bakr RB. Synthesis of chalcones derivatives and their biological activities: A review. ACS Omega. 2022 Aug 16;7(32):27769–86. Available from: .

Details

Primary Language

English

Subjects

Organic Chemical Synthesis

Journal Section

Research Article

Publication Date

November 8, 2026

Submission Date

July 27, 2026

Acceptance Date

October 14, 2026

Published in Issue

Year 2026 Volume: 2026 Number: 2

APA
Ali, R. R., & Beebany, S. (2026). Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives. Journal of the Turkish Chemical Society Section A: Chemistry, 2026(2), 1-9. https://doi.org/10.18596/jotcsa.2004227
AMA
1.Ali RR, Beebany S. Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives. JOTCSA. 2026;2026(2):1-9. doi:10.18596/jotcsa.2004227
Chicago
Ali, Rozhgar Rokan, and Shakhawan Beebany. 2026. “Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives”. Journal of the Turkish Chemical Society Section A: Chemistry 2026 (2): 1-9. https://doi.org/10.18596/jotcsa.2004227.
EndNote
Ali RR, Beebany S (November 1, 2026) Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives. Journal of the Turkish Chemical Society Section A: Chemistry 2026 2 1–9.
IEEE
[1]R. R. Ali and S. Beebany, “Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives”, JOTCSA, vol. 2026, no. 2, pp. 1–9, Nov. 2026, doi: 10.18596/jotcsa.2004227.
ISNAD
Ali, Rozhgar Rokan - Beebany, Shakhawan. “Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives”. Journal of the Turkish Chemical Society Section A: Chemistry 2026/2 (November 1, 2026): 1-9. https://doi.org/10.18596/jotcsa.2004227.
JAMA
1.Ali RR, Beebany S. Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives. JOTCSA. 2026;2026:1–9.
MLA
Ali, Rozhgar Rokan, and Shakhawan Beebany. “Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives”. Journal of the Turkish Chemical Society Section A: Chemistry, vol. 2026, no. 2, Nov. 2026, pp. 1-9, doi:10.18596/jotcsa.2004227.
Vancouver
1.Rozhgar Rokan Ali, Shakhawan Beebany. Preparation, Characterization, Anti-Bacterial Activity and Anti-Oxidation Activity for Some New Benzodioxolyl Pyrazoline Derivatives. JOTCSA. 2026 Nov. 1;2026(2):1-9. doi:10.18596/jotcsa.2004227