Synthesis of Copper Nanoparticles Using a Different Method: Determination of Its Antioxidant and Antimicrobial Activity

Abstract

In this study, it was aimed to obtain copper oxide nanoparticles (CuO NPs) with the method of green synthesis by using peroxidase enzymes which were partly purified from fig (Ficus carica). Copper (II) oxide nanoparticles are successfully synthesized with the green synthesis method on the experiments we performed.  UV-VIS spectroscopy of the characterization of acquired CuO NPs were performed with Scanning Electron Microscopy (SEM) and X-ray Diffraction (XRD). Optimum activation temperature for green synthesis was observed to be in 30 min, pH:8, at 25 ^oC and in the concentration of 1mM CuCl₂. By using peroxidase enzymes with green synthesis, it was found out the results of SEM and XRD measurements that acquired CuO NPs were in the size of 50-120 nm. Afterwards, the antioxidant and antibacterial activities of these nanoparticles were measured and it was understood from the obtained results that CuO NPs had both antioxidant and antimicrobial activities.

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