Voltammetric and spectrophotometric pathways for the determination of total antioxidant capacity in commercial turnip juice

Year 2021, Volume: 8 Issue: 1, 163 - 172, 28.02.2021

Merve Öztürk Ersin Demir Tugba Ozdal

https://doi.org/10.18596/jotcsa.752982

Cited By: 3

Abstract

In this study, total flavonoid substance, total phenolic substance and total antioxidant capacity in turnip juice which made from the turnip (Brassica rape), a plant belonging to the family of Turpgiller (Brassicaceae), and also a drink specific to the Çukurova, were investigated. The turnip juice samples, taken from the turnip juice (A sample) and hot turnip juice (B) sample in local market, prepared in the laboratory were performed as two different parallel. In addition, electrochemical method was used to evaluate total antioxidant capacity (TAC) of turnip juice and hot turnip juice. By the SWSV method, under the optimized experimental conditions was applied for quercetin determination with a limit of detection (LOD) and limit of quantification (LOQ) of 0.017 and 0.057 mg/L, respectively. These values are satisfactory for application to real food samples for the evaluation of TAC. According to the results carried out by spectrophotometric methods, the highest total phenolic and flavonoid contents and TAC were observed as 13.049±13.40 mg GAE/ml, 37.850±0.70 mg QE/ml and 81.831±3,24 mg TE/100ml, respectively. In addition, the TAC in turnip juice and hot turnip juice were calculated as 573.05±0.43 mg/L and 854.98±9.9 mg/L by SWSV, respectively. The results obtained by voltammetry and spectrophotometry methods for determinations of TAC were in correlation and found to be compatible for the determination of TAC.

Keywords

Antioxidant, Turnip Juice, VOltammetry, Spectrophotometry

References

• 1. Bartosz G. Food oxidants and antioxidants: chemical, biological, and functional properties: CRC press; 2013.
• 2. Algarra M, Fernandes A, Mateus N, de Freitas V, da Silva JCE, Casado J. Anthocyanin profile and antioxidant capacity of black carrots (Daucus carota L. ssp. sativus var. atrorubens Alef.) from Cuevas Bajas, Spain. Journal of Food Composition and Analysis. 2014;33(1):71-6.
• 3. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy reviews. 2010;4(8):118.
• 4. Nizamlıoğlu N, Nas S. The phenolic compounds in vegetables and fruit; structures and their importance. Electronic Journal of Food Technologies. 2010;5(1):20-35.
• 5. Erten H, Tanguler H, Canbaş A. A traditional Turkish lactic acid fermented beverage: Shalgam (Salgam). Food Reviews International. 2008;24(3):352-9.
• 6. Tanguler H, Erten H. Occurrence and growth of lactic acid bacteria species during the fermentation of shalgam (salgam), a traditional Turkish fermented beverage. LWT-Food Science and Technology. 2012;46(1):36-41.
• 7. Tanguler H. Identification of predominant lactic acid bacteria isolated from shalgam beverage and improvement of its production technique. Doctor of Philosophy thesis, Cukurova University, Adana, Turkey. 2010.
• 8. Gläßgen WE, Wray V, Strack D, Metzger JW, Seitz HU. Anthocyanins from cell suspension cultures of Daucus carota. Phytochemistry. 1992;31(5):1593-601.
• 9. Alasalvar C, Grigor JM, Zhang D, Quantick PC, Shahidi F. Comparison of volatiles, phenolics, sugars, antioxidant vitamins, and sensory quality of different colored carrot varieties. Journal of Agricultural and Food Chemistry. 2001;49(3):1410-6.
• 10. Wootton-Beard PC, Moran A, Ryan L. Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP, DPPH, ABTS and Folin–Ciocalteu methods. Food Research International. 2011;44(1):217-24.
• 11. Contreras-Calderón J, Calderón-Jaimes L, Guerra-Hernández E, García-Villanova B. Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia. Food research international. 2011;44(7):2047-53.
• 12. Shahidi F, Naczk M. Antioxidant properties of food phenolics. Phenolics in food and nutraceuticals. CRC Press; 2004.
• 13. Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of agricultural and food chemistry. 2002;50(10):3010-4.
• 14. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chemistry. 1999;64(4):555-9.
• 15. Lee KW, Kim YJ, Lee HJ, Lee CY. Cocoa has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine. Journal of agricultural and food chemistry. 2003;51(25):7292-5.
• 16. Kumaran A. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food chemistry. 2006;97(1):109-14.
• 17. Rai S, Wahile A, Mukherjee K, Saha BP, Mukherjee PK. Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds. Journal of ethnopharmacology. 2006;104(3):322-7.
• 18. Apak R, Güçlü K, Özyürek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of agricultural and food chemistry. 2004;52(26):7970-81.
• 19. Demir E, Senocak A, Tassembedo-Koubangoye MF, Demirbas E, Aboul-Enein HY. Electrochemical Evaluation of the Total Antioxidant Capacity of Yam Food Samples on a Polyglycine-Glassy Carbon Modified Electrode. Current Analytical Chemistry. 2018;14:1-8.
• 20. Pastor FT, Šegan DM, Gorjanović SŽ, Kalušević AM, Sužnjević DŽ. Development of voltammetric methods for antioxidant activity determination based on Fe (III) reduction. Microchemical Journal. 2020;155:104721.
• 21. García-Carvajal E, Blandón-Naranjo L, Villa-Montoya V, Vázquez MV, Peláez-Jaramillo C. Electrochemical Approach to the Study of the Antioxidant Capacity of Vaccinium Meridionale Swart at Different Ripening Stages. Portugaliae Electrochimica Acta. 2019;37(1):71-82.
• 22. Alcalde B, Granados M, Saurina J. Exploring the Antioxidant Features of Polyphenols by Spectroscopic and Electrochemical Methods. Antioxidants. 2019;8(11):523.
• 23. Giovagnoli-Vicuña C, Pizarro S, Briones-Labarca V, Delgadillo Á. A Square Wave Voltammetry Study on the Antioxidant Interaction and Effect of Extraction Method for Binary Fruit Mixture Extracts. Journal of Chemistry. 2019;2019.
• 24. de Araújo Rodrigues I, Gomes SM, Fernandes IPG, Oliveira‐Brett AM. Phenolic Composition and Total Antioxidant Capacity by Electrochemical, Spectrophotometric and HPLC‐EC Evaluation in Portuguese Red and White Wines. Electroanalysis. 2019;31(5):936-45.
• 25. Demir E. Sensitive and Selective Pathway of Total Antioxidant Capacity in Commercially Lemon, Watermelon and Mango-pineapple Cold Teas by Square Wave Adsorptive Stripping Voltammetry. Gazi University Journal of Science. 2019;32(4):1123-36.
• 26. Vilas-Boas Â, Valderrama P, Fontes N, Geraldo D, Bento F. Evaluation of total polyphenol content of wines by means of voltammetric techniques: Cyclic voltammetry vs differential pulse voltammetry. Food chemistry. 2019;276:719-25.
• 27. Ekinci FY, Baser GM, Özcan E, Üstündağ ÖG, Korachi M, Sofu A, et al. Characterization of chemical, biological, and antiproliferative properties of fermented black carrot juice, shalgam. European Food Research and Technology. 2016;242(8):1355-68.
• 28. Öztan T. Antioxidant activities and phenolic substance profile of purple carrot, its concentrate, shalgam beverage, pomegranate juice and sour pomegranate concentrate products. International Information System For The Agricultural Science And Technology (AGRIS). 2006.