In vitro antimicrobial, anticancer and antioxidant activities and bioactive contents of endemic Acanthus dioscoridis L. var. dioscoridis flowers from Türkiye

Year 2024, Volume: 7 Issue: 2, 66 - 72, 18.12.2024

Serhat Keser , Omer Kaygılı , Suat Tekin , İsmail Türkoğlu , Prof. Dr. Ökkeş Yılmaz , Mustafa Karatepe , Sevda Kırbağ , Süleyman Sandal

https://doi.org/10.54565/jphcfum.1539481

Abstract

A. dioscoridis is a member of the Acanthaceae family and is represented by approximately 30 species. In Türkiye, 8 species, 6 of which are endemic, are distributed in Eastern and Central Anatolia. In the presented study, the antimicrobial, antiradical, and anticancer properties of flower extracts of endemic A. dioscoridis L. var. dioscoridis were investigated for the first time. The antiradical activity and phytochemical contents of this plant were also investigated. According to our study results, endemic A. dioscoridis flowers extract show anticancer activity against MCF-7, HCT-116 and LNCaP cancer cell lines, high antiradical activity against ABTS radicals, and effective antimicrobial activity against some microorganism-caused infection in humans. In conclusion, this study can be the first report about the anticancer, antiradical, and antimicrobial properties of endemic A. dioscoridis L. var. dioscoridis flower extracts.

Keywords

Acanthus, anticancer, antimicrobial, antioxidant, endemic, phytochemical

Supporting Institution

TÜBİTAK

Project Number

TÜBİTAK 114Z124

Thanks

The present study was supported by TUBITAK, under grant number 114Z124.

References

• Yang SA, Jeon SK, Lee EJ, Shim CH, Lee IS. 2010. Comparative study of the chemical composition and antioxidant activity of six essential oils and their components. Natural Product Research. 24 (2): 140–151.
• Shad AA, Ahmad S, Ullah R, AbdEl-Salam NM, Fouad H, Rehman NU, Hussain H, Saeed W. 2014. Phytochemical and biological activities of four wild medicinal plants. Scientific World Journal. 2014: 857363.
• Abdullah FO, Hamahameen B, Dastan D. 2021. Chemical constituents of the volatile and nonvolatile, cytotoxic and free radical scavenging activities of medicinal plant: Ranunculus millefoliatus and Acanthus dioscoridis. Polish Journal of Environmental Studies. 30: 1981–1989.
• Keser S. 2014. Antiradical activities and phytochemical compounds of firethorn (Pyracantha coccinea) fruit extracts. Natural Product Research. 28: 1789–1794.
• Keser S, Keser F, Kaygili O, Tekin S, Demir E, Turkoglu I, Turkoglu S, Parlak AE, Yilmaz O, Karatepe M, Sandal S, Kirbag S. 2020. Phytochemical compounds and antiradical, antimicrobial, and cytotoxic activities of the extracts from Hypericum scabrum L. flowers. Natural Product Research. 34: 714–719.
• Keser S, Keser F, Karatepe M, Kaygili O, Tekin S, Turkoglu I, Demir E, Yilmaz O, Kirbag S, Sandal, S. 2020. Bioactive contents, in vitro antiradical, antimicrobial and cytotoxic properties of rhubarb (Rheum ribes L.) extracts. Natural Product Research. 34: 3353–3357.
• Keser S, Kak O. 2021. In vitro antimicrobial, antiradical, anticancer evaluation, and phytochemical contents of endemic Scorzonera semicana DC. Journal of Food Processing and Preservation. 45: e15971.
• Çapanlar S. 2008. Phytochemical and biological activity studies on Acanthus hirsutus and Cephalaria paphlagonica species. Master of Science Thesis, Ege University, Graduate School of Natural and Applied Sciences.
• Harput US, Arihan O, Iskit AB, Nagatsu A, Saracoglu I. 2011. Antinociceptive, free-radical scavenging, and cytotoxic activities of Acanthus hirsutus Boiss. Journal of Medicinal Food. 14: 767–774.
• Kumar KTMS, Gorain B, Zothanpuia DKR, Samanta SK, Pal M, Biswas P, Roy A, Adhikari D, Karmakar S, Sen T. 2008. Anti-inflammatory activity of Acanthus ilicifolius. Journal of Ethnopharmacology. 120: 7–12.
• Babu BH. Shylesh BS, Padikkala J. 2001. Antioxidant and hepatoprotective effect of Acanthus ilicifolius. Fitoterapia,.72: 272–277.
• Keskin C. 2017. Investigations of in vitro antioxidant activities, elemental compositions and lipid constituents of Acanthus dioscoridis L. var. dioscoridis L. as a medicinal plant. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi.7:141–148.
• Sing D, Aeri V. 2013. Phytochemical and pharmacological potential of Acanthus ilicifolius. Journal of Pharmacy and Bioallied Sciences. 5: 17–20.
• Amin E, Radwan MM, El-Hawary SS, Fathy MM, Mohammed R, Becnel JJ, Khan I. 2012. Potent insecticidal secondary metabolites from the medicinal plant Acanthus montanus. Records of Natural Products. 6(3): 301–305.
• Huang MY, Zhong LJ, Wang F, Liu QY, Zhang YH. 2014. Chemical constituents from the roots of Acanthus ilicifolius. Biochemical Systematics and Ecology. 55: 145–147.
• Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology. 28: 25–30.
• Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26: 1231–1237.
• Halliwell B, Gutteridge JMC, Aruoma O. 1987. The deoxyribose method: a simple test tube assay for determination of rate constants for reactions of hydroxyl radicals. Analytical Biochemistry. 165: 215–219.
• Slinkard K, Singleton VL. 1977. Total phenol analysis-automation and comparison with manual methods. American Journal of Enology and Viticulture. 28: 49–55.
• Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY. 2003. Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of Agricultural and Food Chemistry. 51: 6509–6515.
• Amaeze OU, Ayoola GA, Sofidiya MO, Adepoju-Bello AA, Adegoke AO, Coker HAB. 2011. Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves. Oxidative Medicine and Cellular Longevity. Article ID 976701, 7 pages.
• Zu YG, Li CY, Fu YJ, Zhao CJ. 2006. Simultaneous determination of catechin, rutin, quercetin, kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaf by RP-HPLC with DAD. Journal of Pharmaceutical and Biomedical Analysis. 41: 714–719.
• Christie WW. 1992. Gas chromatography and lipids. The Oil Press, Glasgow.
• Sanchez-Machado DI, Lopez-Hernandez J, Paseiro-Losado P. 2002. High performance liquid chromatographic determination of alpha-tocopherol in macroalgae. Journal of Chromatography A. 976: 277–284.
• López-Cervantes J, Sánchez-Machado DI, Ríos-Vázquez NJ. 2006 High performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate. Journal of Chromatography A. 1105: 135–139.
• Collins CM, Lyne PM. 1989. Microbiological Methods, Buttermorths-Heinemann, London, England.
• Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 65: 55–63.
• Denizot F, Lang R. 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Immunological Methods. 89: 271–277.