Genome-Wide Analysis of the Cation ATPase Gene Family in Musca domestica L. (Diptera: Muscidae)

Abstract

Musca domestica L. is a significant pest threatening human and animal health by transmitting pathogens and causing substantial economic losses. Widespread resistance to traditional insecticides necessitates the discovery of novel molecular targets for vector control. The Cation ATPase gene family, essential for fundamental physiological processes such as cellular homeostasis, nerve impulse transmission, and osmoregulation, represents a promising target. This study presents a comprehensive genome-wide analysis identifying 36 Cation ATPase genes across the genomes of M. domestica, Aedes albopictus, and Drosophila melanogaster. Gene structures, conserved motifs, and physicochemical properties were thoroughly characterized. Comparative phylogenetic analyses elucidated their evolutionary history and structural divergence. Furthermore, 3D structural modeling, protein-protein interaction networks, and Gene Ontology enrichment analyses were utilized to evaluate their functional properties. In silico annotations indicate that this family plays critical roles in calcium and metal ion homeostasis, alongside the transmembrane transport of inorganic cations. Domain analyses confirmed the presence of P-type ATPase domains, strongly indicating their active participation in processes such as cytosolic calcium regulation. Providing the first comprehensive analysis of the Cation ATPase family in M. domestica, this research establishes a baseline framework for developing innovative and sustainable vector control strategies targeting these genes.

Keywords

• Musca domestica
• Cation ATPase
• Gene family
• Bioinformatic tools.

KATYON ATPaz GEN AİLESİNİN Musca domestica L. (Diptera: Muscidae)’DA GENOM ÇAPINDA ANALİZİ

Abstract

Musca domestica L., patojenleri taşıyarak insan ve hayvan sağlığını tehdit eden ve önemli ekonomik kayıplara neden olan önemli bir zararlıdır. Geleneksel insektisitlere karşı yaygın direnç gelişimi, vektör kontrolü için yeni moleküler hedeflerin keşfedilmesini gerekli kılmaktadır. Hücresel homeostaz, sinir impuls iletimi ve osmoregülasyon gibi temel fizyolojik süreçlerde kritik rol oynayan Katyon ATPaz gen ailesi, umut verici bir hedef olarak öne çıkmaktadır.Bu çalışmada, M. domestica, Aedes albopictus ve Drosophila melanogaster genomlarında toplam 36 Katyon ATPaz geninin tanımlandığı kapsamlı bir genom çapında analiz sunulmaktadır. Gen yapıları, korunmuş motifler ve fizikokimyasal özellikler ayrıntılı olarak karakterize edilmiştir. Karşılaştırmalı filogenetik analizler, bu genlerin evrimsel geçmişini ve yapısal farklılaşmasını ortaya koymuştur. Ayrıca, 3 boyutlu yapısal modelleme, protein-protein etkileşim ağları ve Gen Ontolojisi zenginleştirme analizleri kullanılarak fonksiyonel özellikleri değerlendirilmiştir.In silico anotasyonlar, bu gen ailesinin kalsiyum ve metal iyon homeostazında, ayrıca inorganik katyonların transmembran taşınmasında kritik roller üstlendiğini göstermektedir. Domain analizleri, P-tipi ATPaz domainlerinin varlığını doğrulayarak bu proteinlerin sitozolik kalsiyum düzenlenmesi gibi süreçlerde aktif rol aldığını güçlü şekilde ortaya koymaktadır.M. domestica’da Katyon ATPaz gen ailesine yönelik ilk kapsamlı analizi sunan bu çalışma, bu genleri hedef alan yenilikçi ve sürdürülebilir vektör kontrol stratejilerinin geliştirilmesi için temel bir çerçeve oluşturmaktadır.

Keywords

• Musca domestica
• Katyon ATPaz
• Gen ailesi
• Biyoinformatik araçlar

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