Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum

Mohammad Al-amin; Mohammad Arafat Siddiqui; Shamima Afrin Ruma; Nagla Mustafa Eltayeb; Gazi Nurun Nahar Sultana; Salizawati Muhamad Salhimi; Chowdhury Faiz Hossain

Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum

Abstract

The rhizome of Zingiber roseum has been used as traditional medicines for the treatment of gastric ulcer, skin diseases, and liver infections. Zerumbone is an essential sesquiterpenoid previously isolated from different species of Zingiberaceae and possesses numerous pharmacological properties. Based on previous reports, the aim of the current study was to investigate the antimicrobial activity of extract, fractions, and isolation of zerumbone from Zingiber roseum. The antimicrobial activity was determined using disc diffusion method. The serial tube dilution technique was used to determine Minimum Inhibitory Concentration (MIC) of the samples. Vacuum Liquid Chromatography (VLC) and open column chromatography were used for separation. The methanol extract exhibited maximum antimicrobial activity against Escherichia coli, Saccharomyces cerevisiae, and Aspergillus niger in both methods. VLC of the extract yielded five fractions (Fr. 1 to Fr. 5). Fr. 1 exhibited maximum antimicrobial activity compared to the other fractions. Column chromatography of Fr.1 generated 47 fractions. Fraction 1 to 9 were combined and crystallized in n-hexane to isolate zerumbone as the most abundant source (yield 6.5 % from dried MeOH extract). Zerumbone showed good activity against all tested bacteria. The results of our study might validate the ethnobotanical use of Zingiber roseum rhizomes in the treatment of infectious diseases. Moreover, Zingiber roseum could be one of the most abundant sources for the isolation of natural zerumbone to study different pharmacological studies in the field of new drug discovery.

Keywords

References

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Mohammad Al-amin This is me
Bangladesh

Mohammad Arafat Siddiqui This is me
Bangladesh

Shamima Afrin Ruma This is me
Bangladesh

Nagla Mustafa Eltayeb This is me
Malaysia

Gazi Nurun Nahar Sultana This is me
Bangladesh

Salizawati Muhamad Salhimi This is me
Malaysia

Chowdhury Faiz Hossain ^* This is me
Bangladesh

Publication Date

June 27, 2025

Submission Date

June 8, 2018

Acceptance Date

January 29, 2019

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA38PM84AA

Cite

RIS / Bibtex

APA

Al-amin, M., Siddiqui, M. A., Ruma, S. A., Eltayeb, N. M., Sultana, G. N. N., Muhamad Salhimi, S., & Hossain, C. F. (2025). Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum. Journal of Research in Pharmacy, 23(3), 559-566. https://izlik.org/JA38PM84AA

AMA

1.Al-amin M, Siddiqui MA, Ruma SA, et al. Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum. J. Res. Pharm. 2025;23(3):559-566. https://izlik.org/JA38PM84AA

Chicago

Al-amin, Mohammad, Mohammad Arafat Siddiqui, Shamima Afrin Ruma, et al. 2025. “Antimicrobial Activity of the Crude Extract, Fractions and Isolation of Zerumbone from the Rhizomes of Zingiber Roseum”. Journal of Research in Pharmacy 23 (3): 559-66. https://izlik.org/JA38PM84AA.

EndNote

Al-amin M, Siddiqui MA, Ruma SA, Eltayeb NM, Sultana GNN, Muhamad Salhimi S, Hossain CF (June 1, 2025) Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum. Journal of Research in Pharmacy 23 3 559–566.

IEEE

[1]M. Al-amin et al., “Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum”, J. Res. Pharm., vol. 23, no. 3, pp. 559–566, June 2025, [Online]. Available: https://izlik.org/JA38PM84AA

ISNAD

Al-amin, Mohammad - Siddiqui, Mohammad Arafat - Ruma, Shamima Afrin - Eltayeb, Nagla Mustafa - Sultana, Gazi Nurun Nahar - Muhamad Salhimi, Salizawati - Hossain, Chowdhury Faiz. “Antimicrobial Activity of the Crude Extract, Fractions and Isolation of Zerumbone from the Rhizomes of Zingiber Roseum”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 559-566. https://izlik.org/JA38PM84AA.

JAMA

1.Al-amin M, Siddiqui MA, Ruma SA, Eltayeb NM, Sultana GNN, Muhamad Salhimi S, Hossain CF. Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum. J. Res. Pharm. 2025;23:559–566.

MLA

Al-amin, Mohammad, et al. “Antimicrobial Activity of the Crude Extract, Fractions and Isolation of Zerumbone from the Rhizomes of Zingiber Roseum”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 559-66, https://izlik.org/JA38PM84AA.

Vancouver

1.Mohammad Al-amin, Mohammad Arafat Siddiqui, Shamima Afrin Ruma, Nagla Mustafa Eltayeb, Gazi Nurun Nahar Sultana, Salizawati Muhamad Salhimi, Chowdhury Faiz Hossain. Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):559-66. Available from: https://izlik.org/JA38PM84AA