Development and validation of a UV-Spectrophotometric method using methanol for the simultaneous estimation of doxycycline and voriconazole in pharmaceutical formulations

Year 2025, Volume: 29 Issue: 5, 1792 - 1801, 01.09.2025

Dr. Mrs. Neela Bhatia , Anagha Ajagekar Rutuja Chougale

https://doi.org/10.12991/jrespharm.1763367

Abstract

The development and validation of a UV-spectrophotometric method for the simultaneous estimation of
doxycycline and voriconazole in pharmaceutical formulations are presented in this study. This method was designed
to address the limitations of existing analytical techniques by providing a simple, accurate, and efficient approach
suitable for routine quality control and therapeutic monitoring. Methanol was selected as the solvent, and estimation
was done using the multiwavelength method of analysis. Estimation of doxycycline was done by recording a difference
of absorbance at 358 and 402 nm while the estimation of voriconazole was done by recording absorbance difference
at 428 and 463 nm. The difference of absorbance for estimation of two drugs is recorded for elimination of interference
of the other drug. The method demonstrated excellent linearity, with calibration curves showing strong correlation
coefficients. Accuracy was confirmed through satisfactory recovery rates, and precision was validated with %RSD
values below 2% for both intraday and inter-day measurements. The method exhibited robustness and ruggedness,
maintaining consistent performance under varying experimental conditions and across different analysts. Low limits
of detection and quantification underscored the method's sensitivity. This UV-spectrophotometric method offers
significant practical advantages, including reduced analysis time and cost-effectiveness, making it highly suitable for
adoption in pharmaceutical laboratories. The study concludes that this method is a valuable tool for improving drug
quality and safety, contributing to advancements in pharmaceutical sciences and supporting enhanced productivity in
drug analysis.

Keywords

Simultaneous estimation , doxycycline , voriconazole , method validation , UV-spectrophotometry

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