Formulation of an anti-aging cream containing Alteromonas ferment extract and evaluation of the effect on collagen concentration

Year 2023, Volume: 27 Issue: 2, 903 - 912, 27.06.2025

Gülşah Gedik

Abstract

This study relates to the Alteromonas ferment extract, a by-product of a marine bacteria, Alteromonas
macleodii, is used in anti-aging cream formulation. Alteromonas ferment extract is an innovative anti-pollution
ingredient that chelates heavy metals and has non-toxic anionic polysaccharides, which are used as thickening or
structuring agent in tissue engineering, wound management or drug delivery applications. Aging is a physiological
process, it is undesirable especially on the skin. Aging leads to weakening of facial muscles and loss of subcutaneous
fat and skin laxity, along with wrinkles, prominent lines and sagging skin. Changes in collagen play an important role
in reducing skin elasticity. In this case, fibroblast decreases, matrix metalloproteinase increases (responsible for collagen
breakdown), and glycosaminoglycans decrease. The objective of the study was to evaluate effects of the anti-aging
cosmetic face cream including Alteromonas ferment extract on human dermal fibroblast cells. The outcomes of the
characterization analysis have indicated the development of a successful cream formulation with optimum
characteristics. No microbial growth was observed. Given the results obtained in the tested conditions, the anti- aging
cream is not cytotoxic against fibroblasts 0.5% or less concentration. The dilutions of 0.1% and 0.2% were chosen for
evaluation of collagen concentration, because they were the most appropriate concentrations in terms of solubility and
lack of cytotoxicity. The treatment with anti-aging cream formulation at 0.1% and 0.2% increased collagen synthesis
57% and 67%, respectively in cell culture.

Keywords

Alteromonas ferment extract , anionic polysaccharides , collagen synthesis , anti-aging cream , skin aging

References

• Khan SS, Singer BD, Vaughan DE. Molecular and physiological manifestations and measurement of aging in humans. Aging Cell. 2017;16(4):624–633. https://doi.org/10.1111/acel.12601
• Singh R, Barden A, Mori T, Beilin L. Advanced glycation end-products: A review. Diabetologia. 2001;44(2):129–146. https://doi.org/10.1007/s001250051591
• Friedman O. Changes associated with the aging face. Facial Plast Surg Clin North Am. 2005;13(3):371–380. https://doi.org/10.1016/j.fsc.2005.04.004
• Fore J. A review of skin and the effects of aging on skin structure and function. Ostomy Wound Manage. 2006;52(9):24–35.
• Schroeder P, Lademann J, Darvin M E, Stege H, Marks C, Bruhnke S, Krutmann J. Infrared radiation-induced matrix metalloproteinase in human skin: Implications for protection. J Invest Dermatol. 2008;128(10):2491–2497. https://doi.org/10.1038/jid.2008.116
• Ghersetich I, Lotti T, Campanile G, Grappone C, Dini G. Hyaluronic acid in cutaneous intrinsic aging. Int J Dermatol. 1994;33(2):119–122. https://doi.org/10.1111/j.1365-4362.1994.tb01540.x
• Puri P, Nandar SK, Kathuria S, Ramesh V. Effects of air pollution on the skin: A review. Indian J Dermatol Venereol Leprol. 2017;83(4):415–423. https://doi.org/10.4103/0378-6323.199579
• Risom L, Møller P, Loft S. Oxidative stress-induced DNA damage by particulate air pollution. Mutat Res. 2005; 592(1– 2):119–137. https://doi.org/10.1016/j.mrfmmm.2005.06.012
• Panich U, Sittithumcharee G, Rathviboon N, Jirawatnotai S. Ultraviolet radiation-induced skin aging: the role of DNA damage and oxidative stress in epidermal stem cell damage mediated skin aging. Stem Cells Int. 2016; 7370642. https://doi.org/10.1155/2016/7370642
• Villa MT, White LE, Alam M, Yoo SS, Walton RL. Barbed sutures: a review of the literature. Plast Reconstr Surg. 2008;121(3):102e–108e. https://doi.org/10.1097/01.prs.0000299452.24743.65
• Brandt F, Cazzaniga A, Ballin A. Hyaluronic acid gel fillers in the management of facial aging. Clin Interv Aging. 2008;3(1):153–159. https://doi.org/10.2147/CIA.S2135
• Narins RS, Bowman PH. Injectable skin fillers. Clin Plast Surg. 2005; 32(2):151–162. https://doi.org/10.1016/j.cps.2004.12.002
• Chin J, Jiang BC, Mufidah I, Persada SF, Noer BA. The Investigation of Consumers’ Behavior Intention in Using Green Skincare Products: A Pro-Environmental Behavior Model Approach. Sustainability. 2018; 10(11):3922. https://doi.org/10.3390/su10113922
• Casillo A, Lanzetta R, Parrilli M, Corsaro M M. Exopolysaccharides from marine and marine extremophilic bacteria: structures, properties, ecological roles and applications. Mar. Drugs 2018; 16(2): 69. https://doi.org/10.3390/md16020069
• Shen S, Chen X, Shen Z, Chen H. Marine polysaccharides for wound dressings application: an overview. Pharmaceutics, 2021; 13(10): 1666. https://doi.org/10.3390/pharmaceutics13101666
• Concórdio-Reis P, Ferreira SS, Alves VD, Mopper X, Guézennec J, Coimbra M.A, Reis MAM, Freitas F. Rheological characterization of the exopolysaccharide produced by Alteromonas macleodii mo 169. Int. J. Biol. Macromol. 2023; 227: 619–629. https://doi.org/10.1016/j.ijbiomac.2022.12.117
• Zhang Z, Cai R, Zhang W, Fu Y, Jiao N. A novel exopolysaccharide with metal adsorption capacity produced by a marine bacterium Alteromonas sp. JL2810. Mar. Drugs. 2017; 15(6): 175. https://doi.org/10.3390/md15060175
• Borel M, Lamarque E, Loing E. Unique natural exopolysaccharides for biomimetic protective effect against urban pollution Journal of Cosmetic Science. 2017; 68(1):126–132.
• Garre A, Martinez-Masana G, Piquero-Casals J, Granger C. Redefining face contour with a novel anti-aging cosmetic product: an open-label, prospective clinical study. Clin Cosmet Investig Dermatol. 2017; 13(10):473-482. https://doi.org/10.2147/CCID.S148597
• Wang C, Ga, X, Chen Z, Chen Y, Chen H. Preparation, characterization and application of polysaccharide-based metallic nanoparticles: A review. Polymers. 2017; 9(12): 689. https://doi.org/10.3390/polym9120689
• Concórdio-Reis P, Alves VD, Moppert X, Guézennec J, Freitas F, Reis MAM. Characterization and biotechnological potential of extracellular polysaccharides synthesized by Alteromonas strains isolated from French Polynesia marine environments. Mar. Drugs. 2021; 19(9): 522. https://doi.org/10.3390/md19090522
• Guézennec J, Moppert X, Raguénès G, Richert L, Costa B, Simon-Colin C. Microbial mats in French Polynesia and their biotechnological applications. Process. Biochem. 2011; 46:16–22. https://doi.org/10.1016/j.procbio.2010.09.001
• Shafiq-un-Nabi S, Shakeel F, Talegaonkar S, Ali J, Baboota S, Ahuja A, Khar RK, Ali M. Formulation development and optimization using nanoemulsion technique: A technical note. AAPS PharmSciTech. 2007; 8(2): E12–7. https://doi.org/10.1208/pt0802028
• Terescenco D, Picard C, Clemenceau F, Grisel M, Savary G. Influence of the emollient structure on the properties of cosmetic emulsion containing lamellar liquid crystals. Colloids Surf A Physicochem Eng Asp. 2018; 536: 10–19. https://doi.org/10.1016/j.colsurfa.2017.08.017Get rights and content
• Lukić M, Pantelić I, Savić SD. Towards optimal pH of the skin and topical formulations: From the current state of the art to tailored products. Cosmetics. 2021; 83(3): 69. https://doi.org/10.3390/cosmetics8030069
• Gedik G Alaca S. Evaluation of the effect of anti-pollution & anti-aging eye cream on the collagen contraction. Journal of Pharmaceutical Technology. 2020; 1 (1): 13-17. https://doi.org/10.37662/jpt.2020.2
• Park RM, Ji Ahn JY, Kim SY, Wee JH, Kim YH, Jiho Min J. Effect of alginate oligosaccharides on collagen expression in HS 27 human dermal fibroblasts. J Toxicol Environ Health Sci. 2019; 11: 327-334. https://doi.org/10.1007/s13530-019-0421-5
• Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J. Int. Med. Res. 2015; 37: 1528–1542. https://doi.org/10.1177/147323000903700
• Lee BM, Park SJ, Noh I, Kim CH. The effects of the molecular weights of hyaluronic acid on the immune responses. Biomater Res. 2021; 25: 27. https://doi.org/10.1186/s40824-021-00228-4
• Ruoslahti E, Yamaguchi Y. Proteoglycans as modulators of growth factor activities. Cell. 1991; 64(5):867-9. https://doi.org/10.1016/0092-8674(91)90308-L
• Senni K, Pereira J, Gueniche F, Delbarre-Ladrat C, Sinquin C, Ratiskol J, Godeau G, Fischer AM, Helley D, Colliec- Jouault S. Marine polysaccharides: a source of bioactive molecules for cell therapy and tissue engineering. Mar. Drugs. 2011; 9(9): 1664-1681. https://doi.org/10.3390/md9091664
• Matou S, Colliec-Jouault S, Galy-Fauroux I, Ratiskol J, Sinquin C, Guezennec J, Fischer AM, Helley D. Effect of an oversulfated exopolysaccharide on angiogenesis induced by fibroblast growth factor-2 or vascular endothelial growth factor in vitro. Biochem. Pharmacol. 2005; 69,751–759. https://doi.org/10.1016/j.bcp.2004.11.021
• Ruiz-Velasco C, Baud'Huin M, Duplomb S, Colliec-Jouault S, Heymann D, Padrines M. Effects of a sulphated exopolysaccharide produced by Alteromonas infernus on rat bon cells. Glycobiology. 2011;21(6):781-95. https://doi.org/10.1093/glycob/cwr002
• Sahana TG, Rekha PD. A bioactive exopolysaccharide from marine bacteria Alteromonas sp. PRIM-28 and its role in cell proliferation and wound healing in vitro, International Journal of Biological Macromolecules. 2019; 131: 10-18. https://doi.org/10.1016/j.ijbiomac.2019.03.048
• Singh RP, Shukla MK, Mishra A, Kumari P, Reddy CRK, Jha B. Isolation and characterization of exopolysaccharides from seaweed associated bacteria Bacillus licheniformis. Carbohydr. Polym. 2011; 84(3):1019-1026. https://doi.org/10.1016/j.carbpol.2010.12.061
• Insulkar P, Kerkar S, Lele SS. Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities. Int. J. Biol. Macromol. 2018; 120(Pt B), 1441–1450. https://doi.org/10.1016/j.ijbiomac.2018.09.147
• Jiang Z, Ueno M, Nishiguchi T, Abu R, Isaka S, Okimura T, Yamaguchi K, Oda T. Importance of sulfate groups for the macrophage-stimulating activities of ascophyllan isolated from the brown alga Ascophyllum nodosum. Carbohydr. Res. 2013; 380: 124–129. https://doi.org/10.1016/j.carres.2013.05.018
• Gedik G. Formulation and evaluation of intimate wash containing manuka oil and salix bark extract. J.Res.Pharm. 2022; 26(5): 1252-1260.
• Nazlı H, Gedik G. In-vitro evaluation of dendrimeric formulation of oxaliplatin. Pharm Dev Technol. 2021 Sep;26(7):750-76. https://doi.org/10.1080/10837450.2021.1944205