Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model

Mehmet Mustafa Demir; Volkan İpek; Hidayet Tutun; Harun Çınar; Leyla Elif Özgü Ayözger

doi:10.30782/jrvm.1217557

Research Article

Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model

Year 2023, Volume: 42 Issue: 2, 54 - 64, 31.12.2023

Mehmet Mustafa Demir , Volkan İpek , Hidayet Tutun , Harun Çınar , Leyla Elif Özgü Ayözger

https://doi.org/10.30782/jrvm.1217557

Abstract

This study aimed to evaluate the wound healing activity of an ointment containing the hydroethanolic extract of Salvia Tomentosa (S. tomentosa)
in a dermal wound model using rabbits. Ointments with concentrations of 2.5% (w/v) and 5% (w/v) were prepared using the hydroethanolic
extracts of S. tomentosa. An in vivo excisional wound model was embloyed with New Zealand rabbits (n = 35) to assess the wound healing
activity of these ointments. Rabbits were randomly divided into five experimental groups: negative (received no treatment), vehicle (received
only the ointment vehicle), positive control (treated with Fitokrem®), and those treated with 2.5% (w/v) and 5% (w/v) S. tomentosa ointments.
Wound diameters were measured using calipers on the 4th, 8th, and 14th days, and histopathological examinations were conducted on the 3rd,
7th, and 14th days of treatment. The total phenolic content of the S. tomentosa hydroethanolic extract was determined to be 61.52 ± 2.33 μg of
Gallic Acid Equivalents per mg of dry plant material. The total phenolic content and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity
of the hydroethanolic extract of Salvia tomentosa were determined spectrophotometrically. In terms of wound closure, both
2.5% and 5% S. tomentosa ointments demonstrated wound healing activity comparable to that of Fitokrem®. In conclusion, S. tomentosa exibits a
positive effect on dermal wound healing in rabbits.

Keywords

Rabbit, radical scavenging activity, Salvia tomentosa, total phenolic content, wound healing

Supporting Institution

Burdur Mehmet Akif Ersoy University, Scientific Research Project Office

Project Number

0644-YL-20

Thanks

This work was supported by Burdur Mehmet Akif Ersoy University, Scientific Research Project Office under Grant no. 0644-YL-20. We thank to Prof Dr Osman TUGAY for the herbarium number, and Dr Ümit ERDOĞAN for the ointment preparation and Kale Naturel for the extraction process.

References

1. Kumar S, Wong P, Leaper D. What is new in wound healing? Turkish J Med Sci. 2004;34:147-160.
2. Han G, Ceilley R. Chronic Wound Healing: A Review of Current Management and Treatments. Adv Ther. 2017;34(3):599-610. doi:10.1007/S12325-017-0478-Y
3. Guo S, DiPietro LA. Factors Affecting Wound Healing. J Dent Res. 2010;89(3):219. doi:10.1177/0022034509359125
4. Pereira RF, Bártolo PJ. Traditional Therapies for Skin Wound Healing. Adv Wound Care. 2016;5(5):208. doi:10.1089/WOUND.2013.0506
5. Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res. 2009;37(5):1528-1542. doi:10.1177/147323000903700531
6. Amri B, Martino E, Vitulo F, et al. Marrubium vulgare l. leave extract: Phytochemical composition, antioxidant and wound healing properties. Molecules. 2017;22(11):1851. doi:10.3390/molecules22111851
7. Barku VY. Wound Healing: Contributions from Plant Secondary Metabolite Antioxidants. In: Wound Healing - Current Perspectives. IntechOpen; 2019. doi:10.5772/intechopen.81208
8. Bilir K, Tutun H, Sevin S, Kismali G, Yarsan E. Cytotoxic effects of Rhododendron ponticum L. extract on prostate carcinoma and adenocarcinoma cell line (DU145, PC3). Kafkas Univ Vet Fak Derg. 2018;24(3):451-457.
9. Chah K, Eze C, Emuelosi C, Esimone C. Antibacterial and wound healing properties of methanolic extracts of some Nigerian medicinal plants. J Ethnopharmacol. 2006;104(1-2):164-167. doi:10.1016/J.JEP.2005.08.070
10. Rathore VS, Balachandar K. Three-dimensional finite element simulation of gas tungsten arc welding for β-titanium alloy. Proc Inst Mech Eng Part B J Eng Manuf. 2014;228(7):730-735. doi:10.1177/0954405413513248
11. Kareru PG, Keriko JM, Kenji GM, Thiong’o GT, Gachanja AN, Mukiira HN. Antimicrobial Activities of Skincare Preparations from Plant Extracts. African J Tradit Complement Altern Med. 2010;7(3):214. doi:10.4314/AJTCAM.V7I3.54777
12. Wagate CG, Mbaria JM, Gakuya DW, et al. Screening of some Kenyan medicinal plants for antibacterial activity. Phytother Res. 2010;24(1):150-153. doi:10.1002/PTR.2866
13. Gould SWJ, Fielder MD, Kelly AF, Naughton DP. Anti-microbial activities of pomegranate rind extracts: enhancement by cupric sulphate against clinical isolates of S. aureus, MRSA and PVL positive CA-MSSA. BMC Complement Altern Med. 2009;9. doi:10.1186/1472-6882-9-23
14. Yuniarti WM, Lukiswanto BS. Effects of herbal ointment containing the leaf extracts of Madeira vine (Anredera cordifolia (Ten.) Steenis) for burn wound healing process on albino rats. Vet World. 2017;10(7):808. doi:10.14202/VETWORLD.2017.808-813
15. Rhimi W, Hlel R, Salem I Ben, Boulila A, Rejeb A, Saidi M. Dittrichia viscosa L. Ethanolic Extract Based Ointment with Antiradical, Antioxidant, and Healing Wound Activities. Biomed Res Int. 2019;2019. doi:10.1155/2019/4081253
16. Fikru A, Makonnen E, Eguale T, Debella A, Abie Mekonnen G. Evaluation of in vivo wound healing activity of methanol extract of Achyranthes aspera L. J Ethnopharmacol. 2012;143(2):469-474. doi:10.1016/J. JEP.2012.06.049
17. Haznedaroglu MZ, Karabay NU, Zeybek U. Antibacterial activity of Salvia Tomentosa essential oil. Fitoterapia. 2001;72(7):829-831. doi:10.1016/S0367-326X(01)00335-5
18. Inngjerdingen K, Nergård CS, Diallo D, Mounkoro PP, Paulsen BS. An ethnopharmacological survey of plants used for wound healing in Dogonland, Mali, West Africa. J Ethnopharmacol. 2004;92(2-3):233-244. doi:10.1016/j.jep.2004.02.021
19. Schmidt C, Fronza M, Goettert M, et al. Biological studies on Brazilian plants used in wound healing. J Ethnopharmacol. 2009;122(3):523-532. doi:10.1016/J. JEP.2009.01.022
20. Tümen G, Malyer H, Başer K, Öz Aydın S. Plants used in Anatolia for wound healing. In: Proceedings of the IVth International Congress of Ethnobotany. 2006:217-221.
21. Davis P. Flora of Turkey and the Aegean Islands. Edinburgh University Press; 1982.
22. Arslan M. Cultivation Potential of Salvia Tomentosa and S. aramiensis Under the Eastern Mediterranean Conditions. Sci Pap Ser A Agron. 2016;LIX:174-177.
23. Ulubelen A, Miski M, Neuman P, Mabry TJ. Flavonoids of Salvia Tomentosa (Labiatae). J Nat Prod. 1979;42(3):261-263. doi:10.1021/NP50003A002
24. Askun T, Tumen G, Satil F, Ates M. Characterization of the phenolic composition and antimicrobial activities of Turkish medicinal plants. Pharm Biol. 2009;47(7):563-571. doi:10.1080/13880200902878069
25. Bayan Y, Genç N. Determination of Antifungal and Antioxidant Activities of Salvia Tomentosa Mill. Iğdır Üniversitesi Fen Bilim Enstitüsü Derg. 2017;7(4):17- Demir et al. 2023.
26. Dinçer C, Tontul I, Çam IB, et al. Phenolic composition and antioxidant activity of Salvia Tomentosa Miller: Effects of cultivation, harvesting year, and storage. Turkish J Agric For. 2013;37(5):561-567. doi:10.3906/tar-1211-72
27. Estévez M, Ramírez R, Ventanas S, Cava R. Sage and rosemary essential oils versus BHT for the inhibition of lipid oxidative reactions in liver pâté. LWT - Food Sci Technol. 2007;40(1):58-65. doi:10.1016/j.lwt.2005.07.010
28. Comino-Sanz IM, López-Franco MD, Castro B, Pancorbo-Hidalgo PL. The Role of Antioxidants on Wound Healing: A Review of the Current Evidence. J Clin Med. 2021;10(16). doi:10.3390/JCM10163558
29. Salem FMA, Ibrahim HM. Dry fermented buffalo sausage with sage oil extract: Safety and quality. Grasas y Aceites. 2010;61(1):76-85. doi:10.3989/gya.075109
30. Tepe B, Daferera D, Sokmen A, Sokmen M, Polissiou M. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia Tomentosa Miller (Lamiaceae). Food Chem. 2005;90(3):333-340. doi:10.1016/j.foodchem.2003.09.013
31. Scrima M, Melito C, Merola F, et al. Evaluation of wound healing activity of salvia haenkei hydroalcoholic aerial part extract on in vitro and in vivo experimental models. Clin Cosmet Investig Dermatol. 2020;13:627-637. doi:10.2147/CCID.S224418
32. Pawar SS, Dasgupta D. Quantification of phenolic content from stem-bark and root of Hugonia mystax Linn. using RP-HPLC. J King Saud Univ - Sci. 2018;30(3):293-300. doi:10.1016/J.JKSUS.2016.09.002
33. Ben Mansour R, Ksouri WM, Cluzet S, Krisa S, Richard T, Ksouri R. Assessment of Antioxidant Activity and Neuroprotective Capacity on PC12 Cell Line of Frankenia thymifolia and Related Phenolic LC-MS/MS Identification. Evidence-based Complement Altern Med. 2016;2016. doi:10.1155/2016/2843463
34. Huang LP, Wang GQ, Jia ZS, Chen JW, Wang G, Wang XL. Paclitaxel reduces formation of hypertrophic scars in the rabbit ear model. Ther Clin Risk Manag. 2015;11:1089. doi:10.2147/TCRM.S82961
35. Ahmed RR, Mahmoud A, Ahmed OM, Metwalli A, Ebaid H. Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats. Biol Res. 2015;48(1):1-12. doi:10.1186/S40659-015- 0044-5/TABLES/4
36. Gül Satar NY, Cangül IT, Topal A, Kurt H, Ipek V, Onel GI. The effects of Tarantula cubensis venom on open wound healing in rats. J Wound Care. 2017;26(2):66-71. doi:10.12968/jowc.2017.26.2.66
37. Güzel S, Özay Y, Kumaş M, et al. Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats. Biomed Pharmacother. 2019;111(September 2018):1260-1276. doi:10.1016/j.biopha.2019.01.038
38. Broughton G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg. 2006;117(7 SUPPL.). doi:10.1097/01.prs.0000225430.42531.c2
39. Budovsky A, Yarmolinsky L, Ben-Shabat S. Effect of medicinal plants on wound healing. Wound Repair Regen. 2015;23(2):171-183. doi:10.1111/wrr.12274
40. Tan N, Satana D, Sen B, et al. Antimycobacterial and antifungal activities of selected four Salvia species. Rec Nat Prod. 2016;10(5):593-603.
41. Ulukanli Z, Karabörklü S, Cenet M, Sagdic O, Ozturk I, Balcilar M. Essential oil composition, insecticidal and antibacterial activities of Salvia Tomentosa Miller. Med Chem Res. 2013;22(2):832-840. doi:10.1007/s00044-012-0075-1
42. Gruenwald J, Brendler T, Jaenicke C. PDR for Herbal Medicines. Thompson PDR; 2000.
43. Tuladhar ET, Rao A. Plasma protein oxidation and total antioxidant power in premenstrual syndrome. Asian Pac J Trop Med. 2010;3(3):237-240. doi:10.1016/ S1995-7645(10)60017-X
44. Wojdyło A, Oszmiański J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 2007;105(3):940-949. doi:10.1016/J. FOODCHEM.2007.04.038
45. Khorasani Esmaeili A, Mat Taha R, Mohajer S, Banisalam B. Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In vivo and In vitro Grown Trifolium pratense L. (Red Clover). Biomed Res Int. 2015;2015. doi:10.1155/2015/643285
46. Erdogan-Orhan I, Baki E, Şenol S, Yilmaz G. Sagecalled plant species sold in Turkey and their antioxidant activities. J Serbian Chem Soc. 2010;75(11):1491- 1501. doi:10.2298/JSC100322115E
47. Firuzi O, Miri R, Asadollahi M, Eslami S, Jassbi AR. Cytotoxic, Antioxidant and Antimicrobial Activities and Phenolic Contents of Eleven Salvia Species from Iran. Iran J Pharm Res IJPR. 2013;12(4):801.
48. Agar O, Dikmen M, Ozturk N, Yilmaz M, Temel H, Turkmenoglu F. Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey. Molecules. 2015;20(10):17976-18000. doi:10.3390/MOLECULES201017976
49. Aker D, Nisbet C. Antioxidant activities, total phenolic and flavonoid contents of honey collected from different botanical origins. Ankara Üniversitesi Vet Fakültesi Derg. 2020;67(2):133-136. doi:10.33988/AUVFD. 523745
50. Erturk Ö, Kalın S, Ayvaz MÇ. Physicochemical properties, bioactive components, antioxidant and antimicrobial potentials of some selected honeys from different provinces of Turkey. Br Food J. 2019;121(6):1298- 1313. doi:10.1108/BFJ-04-2018-0261
51. Schäfer M, Werner S. Oxidative stress in normal and impaired wound repair. Pharmacol Res. 2008;58(2):165-171. doi:10.1016/J.PHRS.2008.06.004
52. Yadav E, Singh D, Yadav P, Verma A. Antioxidant and anti-inflammatory properties of Prosopis cineraria based phenolic rich ointment in wound healing. Biomed Pharmacother. 2018;108:1572-1583. doi:10.1016/J.BIOPHA.2018.09.180
53. Jagetia GC, Rajanikant GK. Role of curcumin, a naturally occurring phenolic compound of turmeric in accelerating the repair of excision wound, in mice whole-body exposed to various doses of γ-radiation. J Surg Res. 2004;120(1):127-138. doi:10.1016/j. jss.2003.12.003
54. Guimarães I, Baptista-Silva S, Pintado M, Oliveira AL. Polyphenols: A Promising Avenue in Therapeutic Solutions for Wound Care. Appl Sci 2021, Vol 11, Page 1230. 2021;11(3):1230. doi:10.3390/APP11031230
55. Karimzadeh S, Farahpour MR. Topical application of Salvia officinalis hydroethanolic leaf extract improves wound healing process. Indian J Exp Biol. 2017;55(2):98-106.
56. Pintapagung T, Asawapattanakul T, Buasrithong N, Phunnanon M, Thaingkhan W. Effect of oral administration of chia (Salvia hispanica L.) seed extract on wound healing property in diabetic mice. Vet Integr Sci. 2020;18(3):153-171.
57. Irmak F, Kurt Yazar S, Şirvan SS, Serin M, Özağarı A, Karasoy Yeşilada A. Beneficial effects of Salvia miltiorrhiza in the healing of burn wounds: an experimental study in rats. J Plast Surg Hand Surg. 2018;52(4):229- 233. doi:10.1080/2000656X.2018.1461631
58. Salimikia I, Aryanpour M, Bahramsoltani R, et al. Phytochemical and Wound Healing Effects of Methanolic Extract of Salvia Multicaulis Vahl. in Rat. J Med Plants Stud. 2016;15(57); 38-46.
59. Süntar I, Akkol EK, Enol FS, Keles H, Orhan IE. Investigating wound healing, tyrosinase inhibitory and antioxidant activities of the ethanol extracts of Salvia cryptantha and Salvia cyanescens using in vivo and in vitro experimental models. J Ethnopharmacol. 2011;135(1):71-77. doi:10.1016/j.jep.2011.02.022
60. Pastar I, Stojadinovic O, Yin NC, et al. Epithelialization in Wound Healing: A Comprehensive Review. Adv Wound Care. 2014;3(7):445-464. doi:10.1089/wound.2013.0473
61. Ben Amar M, Wu M. Re-epithelialization: advancing epithelium frontier during wound healing. J R Soc Interface. 2014;11(93):20131038. doi:10.1098/rsif.2013.1038
62. Duque GA, Descoteaux A. Macrophage cytokines: Involvement in immunity and infectious diseases. Front Immunol. 2014;5(OCT):491. doi:10.3389/fimmu.2014.00491
63. Farahpour MR, Mirzakhani N, Doostmohammadi J, Ebrahimzadeh M. Hydroethanolic Pistacia atlantica hulls extract improved wound healing process; evidence for mast cells infiltration, angiogenesis and RNA stability. Int J Surg. 2015;17:88-98. doi:10.1016/j.ijsu.2015.03.019
64. Harper D, Young A, McNaught CE. The physiology of wound healing. Surg (United Kingdom). 2014;32(9):445 450. doi:10.1016/j.mpsur.2014.06.010
65. Cerqueira MT, Pirraco RP, Martins AR, Santos TC, Reis RL, Marques AP. Cell sheet technology-driven re epithelialization and neovascularization of skin wounds. Acta Biomater. 2014;10(7):3145-3155. doi:10.1016/j. actbio.2014.03.006
66. Ackermann MR. Inflammation and Healing. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th ed. Elsevier; 2017:121-131.
67. Askun T, Hüsnü Can Başer K, Tümen G, Kürkçüoğlu M. Characterization of essential oils of some Salvia species and their antimycobacterial activities. Turkish J Biol. 2010;34(1):89-95. doi:10.3906/biy-0809-2
68. Sawmiller D, Li S, Shahaduzzaman M, et al. Luteolin reduces Alzheimer’s disease pathologies induced by traumatic brain injury. Int J Mol Sci. 2014;15(1):895-904. doi:10.3390/ijms15010895

Year 2023, Volume: 42 Issue: 2, 54 - 64, 31.12.2023

Mehmet Mustafa Demir , Volkan İpek , Hidayet Tutun , Harun Çınar , Leyla Elif Özgü Ayözger

https://doi.org/10.30782/jrvm.1217557

Abstract

Keywords

Rabbit, radical scavenging activity, Salvia tomentosa, total phenolic content, wound healing

Project Number

0644-YL-20

References

1. Kumar S, Wong P, Leaper D. What is new in wound healing? Turkish J Med Sci. 2004;34:147-160.
2. Han G, Ceilley R. Chronic Wound Healing: A Review of Current Management and Treatments. Adv Ther. 2017;34(3):599-610. doi:10.1007/S12325-017-0478-Y
3. Guo S, DiPietro LA. Factors Affecting Wound Healing. J Dent Res. 2010;89(3):219. doi:10.1177/0022034509359125
4. Pereira RF, Bártolo PJ. Traditional Therapies for Skin Wound Healing. Adv Wound Care. 2016;5(5):208. doi:10.1089/WOUND.2013.0506
5. Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res. 2009;37(5):1528-1542. doi:10.1177/147323000903700531
6. Amri B, Martino E, Vitulo F, et al. Marrubium vulgare l. leave extract: Phytochemical composition, antioxidant and wound healing properties. Molecules. 2017;22(11):1851. doi:10.3390/molecules22111851
7. Barku VY. Wound Healing: Contributions from Plant Secondary Metabolite Antioxidants. In: Wound Healing - Current Perspectives. IntechOpen; 2019. doi:10.5772/intechopen.81208
8. Bilir K, Tutun H, Sevin S, Kismali G, Yarsan E. Cytotoxic effects of Rhododendron ponticum L. extract on prostate carcinoma and adenocarcinoma cell line (DU145, PC3). Kafkas Univ Vet Fak Derg. 2018;24(3):451-457.
9. Chah K, Eze C, Emuelosi C, Esimone C. Antibacterial and wound healing properties of methanolic extracts of some Nigerian medicinal plants. J Ethnopharmacol. 2006;104(1-2):164-167. doi:10.1016/J.JEP.2005.08.070
10. Rathore VS, Balachandar K. Three-dimensional finite element simulation of gas tungsten arc welding for β-titanium alloy. Proc Inst Mech Eng Part B J Eng Manuf. 2014;228(7):730-735. doi:10.1177/0954405413513248
11. Kareru PG, Keriko JM, Kenji GM, Thiong’o GT, Gachanja AN, Mukiira HN. Antimicrobial Activities of Skincare Preparations from Plant Extracts. African J Tradit Complement Altern Med. 2010;7(3):214. doi:10.4314/AJTCAM.V7I3.54777
12. Wagate CG, Mbaria JM, Gakuya DW, et al. Screening of some Kenyan medicinal plants for antibacterial activity. Phytother Res. 2010;24(1):150-153. doi:10.1002/PTR.2866
13. Gould SWJ, Fielder MD, Kelly AF, Naughton DP. Anti-microbial activities of pomegranate rind extracts: enhancement by cupric sulphate against clinical isolates of S. aureus, MRSA and PVL positive CA-MSSA. BMC Complement Altern Med. 2009;9. doi:10.1186/1472-6882-9-23
14. Yuniarti WM, Lukiswanto BS. Effects of herbal ointment containing the leaf extracts of Madeira vine (Anredera cordifolia (Ten.) Steenis) for burn wound healing process on albino rats. Vet World. 2017;10(7):808. doi:10.14202/VETWORLD.2017.808-813
15. Rhimi W, Hlel R, Salem I Ben, Boulila A, Rejeb A, Saidi M. Dittrichia viscosa L. Ethanolic Extract Based Ointment with Antiradical, Antioxidant, and Healing Wound Activities. Biomed Res Int. 2019;2019. doi:10.1155/2019/4081253
16. Fikru A, Makonnen E, Eguale T, Debella A, Abie Mekonnen G. Evaluation of in vivo wound healing activity of methanol extract of Achyranthes aspera L. J Ethnopharmacol. 2012;143(2):469-474. doi:10.1016/J. JEP.2012.06.049
17. Haznedaroglu MZ, Karabay NU, Zeybek U. Antibacterial activity of Salvia Tomentosa essential oil. Fitoterapia. 2001;72(7):829-831. doi:10.1016/S0367-326X(01)00335-5
18. Inngjerdingen K, Nergård CS, Diallo D, Mounkoro PP, Paulsen BS. An ethnopharmacological survey of plants used for wound healing in Dogonland, Mali, West Africa. J Ethnopharmacol. 2004;92(2-3):233-244. doi:10.1016/j.jep.2004.02.021
19. Schmidt C, Fronza M, Goettert M, et al. Biological studies on Brazilian plants used in wound healing. J Ethnopharmacol. 2009;122(3):523-532. doi:10.1016/J. JEP.2009.01.022
20. Tümen G, Malyer H, Başer K, Öz Aydın S. Plants used in Anatolia for wound healing. In: Proceedings of the IVth International Congress of Ethnobotany. 2006:217-221.
21. Davis P. Flora of Turkey and the Aegean Islands. Edinburgh University Press; 1982.
22. Arslan M. Cultivation Potential of Salvia Tomentosa and S. aramiensis Under the Eastern Mediterranean Conditions. Sci Pap Ser A Agron. 2016;LIX:174-177.
23. Ulubelen A, Miski M, Neuman P, Mabry TJ. Flavonoids of Salvia Tomentosa (Labiatae). J Nat Prod. 1979;42(3):261-263. doi:10.1021/NP50003A002
24. Askun T, Tumen G, Satil F, Ates M. Characterization of the phenolic composition and antimicrobial activities of Turkish medicinal plants. Pharm Biol. 2009;47(7):563-571. doi:10.1080/13880200902878069
25. Bayan Y, Genç N. Determination of Antifungal and Antioxidant Activities of Salvia Tomentosa Mill. Iğdır Üniversitesi Fen Bilim Enstitüsü Derg. 2017;7(4):17- Demir et al. 2023.
26. Dinçer C, Tontul I, Çam IB, et al. Phenolic composition and antioxidant activity of Salvia Tomentosa Miller: Effects of cultivation, harvesting year, and storage. Turkish J Agric For. 2013;37(5):561-567. doi:10.3906/tar-1211-72
27. Estévez M, Ramírez R, Ventanas S, Cava R. Sage and rosemary essential oils versus BHT for the inhibition of lipid oxidative reactions in liver pâté. LWT - Food Sci Technol. 2007;40(1):58-65. doi:10.1016/j.lwt.2005.07.010
28. Comino-Sanz IM, López-Franco MD, Castro B, Pancorbo-Hidalgo PL. The Role of Antioxidants on Wound Healing: A Review of the Current Evidence. J Clin Med. 2021;10(16). doi:10.3390/JCM10163558
29. Salem FMA, Ibrahim HM. Dry fermented buffalo sausage with sage oil extract: Safety and quality. Grasas y Aceites. 2010;61(1):76-85. doi:10.3989/gya.075109
30. Tepe B, Daferera D, Sokmen A, Sokmen M, Polissiou M. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia Tomentosa Miller (Lamiaceae). Food Chem. 2005;90(3):333-340. doi:10.1016/j.foodchem.2003.09.013
31. Scrima M, Melito C, Merola F, et al. Evaluation of wound healing activity of salvia haenkei hydroalcoholic aerial part extract on in vitro and in vivo experimental models. Clin Cosmet Investig Dermatol. 2020;13:627-637. doi:10.2147/CCID.S224418
32. Pawar SS, Dasgupta D. Quantification of phenolic content from stem-bark and root of Hugonia mystax Linn. using RP-HPLC. J King Saud Univ - Sci. 2018;30(3):293-300. doi:10.1016/J.JKSUS.2016.09.002
33. Ben Mansour R, Ksouri WM, Cluzet S, Krisa S, Richard T, Ksouri R. Assessment of Antioxidant Activity and Neuroprotective Capacity on PC12 Cell Line of Frankenia thymifolia and Related Phenolic LC-MS/MS Identification. Evidence-based Complement Altern Med. 2016;2016. doi:10.1155/2016/2843463
34. Huang LP, Wang GQ, Jia ZS, Chen JW, Wang G, Wang XL. Paclitaxel reduces formation of hypertrophic scars in the rabbit ear model. Ther Clin Risk Manag. 2015;11:1089. doi:10.2147/TCRM.S82961
35. Ahmed RR, Mahmoud A, Ahmed OM, Metwalli A, Ebaid H. Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats. Biol Res. 2015;48(1):1-12. doi:10.1186/S40659-015- 0044-5/TABLES/4
36. Gül Satar NY, Cangül IT, Topal A, Kurt H, Ipek V, Onel GI. The effects of Tarantula cubensis venom on open wound healing in rats. J Wound Care. 2017;26(2):66-71. doi:10.12968/jowc.2017.26.2.66
37. Güzel S, Özay Y, Kumaş M, et al. Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats. Biomed Pharmacother. 2019;111(September 2018):1260-1276. doi:10.1016/j.biopha.2019.01.038
38. Broughton G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg. 2006;117(7 SUPPL.). doi:10.1097/01.prs.0000225430.42531.c2
39. Budovsky A, Yarmolinsky L, Ben-Shabat S. Effect of medicinal plants on wound healing. Wound Repair Regen. 2015;23(2):171-183. doi:10.1111/wrr.12274
40. Tan N, Satana D, Sen B, et al. Antimycobacterial and antifungal activities of selected four Salvia species. Rec Nat Prod. 2016;10(5):593-603.
41. Ulukanli Z, Karabörklü S, Cenet M, Sagdic O, Ozturk I, Balcilar M. Essential oil composition, insecticidal and antibacterial activities of Salvia Tomentosa Miller. Med Chem Res. 2013;22(2):832-840. doi:10.1007/s00044-012-0075-1
42. Gruenwald J, Brendler T, Jaenicke C. PDR for Herbal Medicines. Thompson PDR; 2000.
43. Tuladhar ET, Rao A. Plasma protein oxidation and total antioxidant power in premenstrual syndrome. Asian Pac J Trop Med. 2010;3(3):237-240. doi:10.1016/ S1995-7645(10)60017-X
44. Wojdyło A, Oszmiański J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 2007;105(3):940-949. doi:10.1016/J. FOODCHEM.2007.04.038
45. Khorasani Esmaeili A, Mat Taha R, Mohajer S, Banisalam B. Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In vivo and In vitro Grown Trifolium pratense L. (Red Clover). Biomed Res Int. 2015;2015. doi:10.1155/2015/643285
46. Erdogan-Orhan I, Baki E, Şenol S, Yilmaz G. Sagecalled plant species sold in Turkey and their antioxidant activities. J Serbian Chem Soc. 2010;75(11):1491- 1501. doi:10.2298/JSC100322115E
47. Firuzi O, Miri R, Asadollahi M, Eslami S, Jassbi AR. Cytotoxic, Antioxidant and Antimicrobial Activities and Phenolic Contents of Eleven Salvia Species from Iran. Iran J Pharm Res IJPR. 2013;12(4):801.
48. Agar O, Dikmen M, Ozturk N, Yilmaz M, Temel H, Turkmenoglu F. Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey. Molecules. 2015;20(10):17976-18000. doi:10.3390/MOLECULES201017976
49. Aker D, Nisbet C. Antioxidant activities, total phenolic and flavonoid contents of honey collected from different botanical origins. Ankara Üniversitesi Vet Fakültesi Derg. 2020;67(2):133-136. doi:10.33988/AUVFD. 523745
50. Erturk Ö, Kalın S, Ayvaz MÇ. Physicochemical properties, bioactive components, antioxidant and antimicrobial potentials of some selected honeys from different provinces of Turkey. Br Food J. 2019;121(6):1298- 1313. doi:10.1108/BFJ-04-2018-0261
51. Schäfer M, Werner S. Oxidative stress in normal and impaired wound repair. Pharmacol Res. 2008;58(2):165-171. doi:10.1016/J.PHRS.2008.06.004
52. Yadav E, Singh D, Yadav P, Verma A. Antioxidant and anti-inflammatory properties of Prosopis cineraria based phenolic rich ointment in wound healing. Biomed Pharmacother. 2018;108:1572-1583. doi:10.1016/J.BIOPHA.2018.09.180
53. Jagetia GC, Rajanikant GK. Role of curcumin, a naturally occurring phenolic compound of turmeric in accelerating the repair of excision wound, in mice whole-body exposed to various doses of γ-radiation. J Surg Res. 2004;120(1):127-138. doi:10.1016/j. jss.2003.12.003
54. Guimarães I, Baptista-Silva S, Pintado M, Oliveira AL. Polyphenols: A Promising Avenue in Therapeutic Solutions for Wound Care. Appl Sci 2021, Vol 11, Page 1230. 2021;11(3):1230. doi:10.3390/APP11031230
55. Karimzadeh S, Farahpour MR. Topical application of Salvia officinalis hydroethanolic leaf extract improves wound healing process. Indian J Exp Biol. 2017;55(2):98-106.
56. Pintapagung T, Asawapattanakul T, Buasrithong N, Phunnanon M, Thaingkhan W. Effect of oral administration of chia (Salvia hispanica L.) seed extract on wound healing property in diabetic mice. Vet Integr Sci. 2020;18(3):153-171.
57. Irmak F, Kurt Yazar S, Şirvan SS, Serin M, Özağarı A, Karasoy Yeşilada A. Beneficial effects of Salvia miltiorrhiza in the healing of burn wounds: an experimental study in rats. J Plast Surg Hand Surg. 2018;52(4):229- 233. doi:10.1080/2000656X.2018.1461631
58. Salimikia I, Aryanpour M, Bahramsoltani R, et al. Phytochemical and Wound Healing Effects of Methanolic Extract of Salvia Multicaulis Vahl. in Rat. J Med Plants Stud. 2016;15(57); 38-46.
59. Süntar I, Akkol EK, Enol FS, Keles H, Orhan IE. Investigating wound healing, tyrosinase inhibitory and antioxidant activities of the ethanol extracts of Salvia cryptantha and Salvia cyanescens using in vivo and in vitro experimental models. J Ethnopharmacol. 2011;135(1):71-77. doi:10.1016/j.jep.2011.02.022
60. Pastar I, Stojadinovic O, Yin NC, et al. Epithelialization in Wound Healing: A Comprehensive Review. Adv Wound Care. 2014;3(7):445-464. doi:10.1089/wound.2013.0473
61. Ben Amar M, Wu M. Re-epithelialization: advancing epithelium frontier during wound healing. J R Soc Interface. 2014;11(93):20131038. doi:10.1098/rsif.2013.1038
62. Duque GA, Descoteaux A. Macrophage cytokines: Involvement in immunity and infectious diseases. Front Immunol. 2014;5(OCT):491. doi:10.3389/fimmu.2014.00491
63. Farahpour MR, Mirzakhani N, Doostmohammadi J, Ebrahimzadeh M. Hydroethanolic Pistacia atlantica hulls extract improved wound healing process; evidence for mast cells infiltration, angiogenesis and RNA stability. Int J Surg. 2015;17:88-98. doi:10.1016/j.ijsu.2015.03.019
64. Harper D, Young A, McNaught CE. The physiology of wound healing. Surg (United Kingdom). 2014;32(9):445 450. doi:10.1016/j.mpsur.2014.06.010
65. Cerqueira MT, Pirraco RP, Martins AR, Santos TC, Reis RL, Marques AP. Cell sheet technology-driven re epithelialization and neovascularization of skin wounds. Acta Biomater. 2014;10(7):3145-3155. doi:10.1016/j. actbio.2014.03.006
66. Ackermann MR. Inflammation and Healing. In: Zachary JF, ed. Pathologic Basis of Veterinary Disease. 6th ed. Elsevier; 2017:121-131.
67. Askun T, Hüsnü Can Başer K, Tümen G, Kürkçüoğlu M. Characterization of essential oils of some Salvia species and their antimycobacterial activities. Turkish J Biol. 2010;34(1):89-95. doi:10.3906/biy-0809-2
68. Sawmiller D, Li S, Shahaduzzaman M, et al. Luteolin reduces Alzheimer’s disease pathologies induced by traumatic brain injury. Int J Mol Sci. 2014;15(1):895-904. doi:10.3390/ijms15010895

There are 68 citations in total.

Details

Primary Language	English
Subjects	Veterinary Sciences (Other)
Journal Section	Research Articles
Authors	Mehmet Mustafa Demir 0000-0001-9953-6355 Volkan İpek 0000-0001-5874-7797 Hidayet Tutun 0000-0001-9512-8637 Harun Çınar 0000-0003-4412-8949 Leyla Elif Özgü Ayözger 0000-0003-3320-1688
Project Number	0644-YL-20
Publication Date	December 31, 2023
Acceptance Date	October 13, 2023
Published in Issue	Year 2023 Volume: 42 Issue: 2

Cite

APA	Demir, M. M., İpek, V., Tutun, H., Çınar, H., et al. (2023). Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model. Journal of Research in Veterinary Medicine, 42(2), 54-64. https://doi.org/10.30782/jrvm.1217557
AMA	Demir MM, İpek V, Tutun H, Çınar H, Ayözger LEÖ. Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model. J Res Vet Med. December 2023;42(2):54-64. doi:10.30782/jrvm.1217557
Chicago	Demir, Mehmet Mustafa, Volkan İpek, Hidayet Tutun, Harun Çınar, and Leyla Elif Özgü Ayözger. “Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model”. Journal of Research in Veterinary Medicine 42, no. 2 (December 2023): 54-64. https://doi.org/10.30782/jrvm.1217557.
EndNote	Demir MM, İpek V, Tutun H, Çınar H, Ayözger LEÖ (December 1, 2023) Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model. Journal of Research in Veterinary Medicine 42 2 54–64.
IEEE	M. M. Demir, V. İpek, H. Tutun, H. Çınar, and L. E. Ö. Ayözger, “Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model”, J Res Vet Med, vol. 42, no. 2, pp. 54–64, 2023, doi: 10.30782/jrvm.1217557.
ISNAD	Demir, Mehmet Mustafa et al. “Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model”. Journal of Research in Veterinary Medicine 42/2 (December 2023), 54-64. https://doi.org/10.30782/jrvm.1217557.
JAMA	Demir MM, İpek V, Tutun H, Çınar H, Ayözger LEÖ. Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model. J Res Vet Med. 2023;42:54–64.
MLA	Demir, Mehmet Mustafa et al. “Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model”. Journal of Research in Veterinary Medicine, vol. 42, no. 2, 2023, pp. 54-64, doi:10.30782/jrvm.1217557.
Vancouver	Demir MM, İpek V, Tutun H, Çınar H, Ayözger LEÖ. Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model. J Res Vet Med. 2023;42(2):54-6.

Download Cover Image

Article Files

Full Text