Zeytinyağı Değirmeni Atıksuyundan Yarrowia lipolytica ile Biyokütle Üretimi: Protein İçeriğinin Değerlendirilmesi

Year 2024, Volume: 2 Issue: 2, 136 - 143, 31.12.2024

Bilge Sayın

https://doi.org/10.63063/jsat.1596989

Abstract

Zeytinyağı üretiminin bir yan ürünü olan zeytin değirmeni atık suyu (ZDA), mikrobiyal uygulamalar için umut vadeden bir substrattır. Bu tarımsal-endüstriyel atık, mikrobiyal büyümeyi destekleyen karbon ve azot kaynakları içerdiğinden biyokütle üretimi için önemli bir potansiyel sunmaktadır. Ayrıca, bu atığın kullanımı çevresel olarak sürdürülebilir bir atık yönetimine katkıda bulunmaktadır. ZDA’yı bir büyüme ortamı olarak kullanan Yarrowia lipolytica, sentetik ortama bir alternatif sunarak bu atığın ekonomik değerini artırırken üretim maliyetlerini düşürmektedir. Bu çalışmada, Y. lipolytica tarafından ZDA’dan biyokütle üretimi Taguchi yöntemi kullanılarak optimize edilmiştir. Deneysel tasarım, OMW seyreltme oranının (%20, 40 ve 60), azot konsantrasyonunun (0, 1 ve 2 g/L) ve inkübasyon süresinin (3, 5 ve 7 gün) etkilerini değerlendirmiştir. L9 ortogonal dizilimi kullanılarak dokuz deney yürütülmüş ve önemli faktörler varyans analizi (ANOVA) kullanılarak belirlenmiştir. Bu değişkenler arasında azot konsantrasyonu biyokütle üretimini önemli ölçüde etkilemiştir (p < 0,05). En yüksek biyokütle konsantrasyonu 11,58±0,93 g/L olarak elde edilmiş ve optimum koşullar %60 OMW seyreltme oranı, 2 g/L azot ilavesi ve 5 gün inkübasyon olarak bulunmuştur. Bu koşullar altında, biyokütlenin protein içeriği %25,88±0,63 w/w biyokütle kuru ağırlığı olarak belirlenmiştir.

Keywords

zeytin değirmeni atık suyu, biyokütle, Yarrowia, atık yönetimi, Taguchi

Biomass Production by Yarrowia lipolytica from Olive Mill Wastewater: Evaluation of Protein Content

Abstract

Olive mill wastewater (OMW), a by-product of olive oil production, is a promising substrate for microbial applications. This agro-industrial waste offers considerable potential for biomass production as it contains carbon and nitrogen sources that support microbial growth. Additionally, utilization of this waste contributes to environmentally sustainable waste management. Utilizing OMW as a growth medium, Yarrowia lipolytica offers an alternative to synthetic media, enhancing the economic value of this waste while reducing production costs. In this study, biomass production by Y. lipolytica from OMW was optimized using the Taguchi method. The experimental design evaluated the effects of the OMW dilution rate (20, 40, and 60%), nitrogen concentration (0, 1, and 2 g/L), and incubation time (3, 5, and 7 days). Nine experiments were conducted using the L9 orthogonal array, and significant factors were identified using analysis of variance (ANOVA). Among these variables, nitrogen concentration significantly influenced biomass production (p < 0.05). The highest biomass concentration of 11.58±0.93 g/L was achieved, and the optimum conditions were found as OMW dilution rate of 60%, 2 g/L nitrogen addition, and 5 days of incubation. Under these conditions, the protein content of the biomass was determined as 25.88±0.63% w/w biomass dry weight.

Keywords

olive mill wastewater, biomass, Yarrowia, waste management, Taguchi

References

• D. Sarris, A. Rapti, N. Papafotis, A. A. Koutinas, and S. Papanikolaou, "Production of added-value chemical compounds through bioconversions of olive-mill wastewaters blended with crude glycerol by a Yarrowia lipolytica strain," Molecules, vol. 24, no. 2, p. 222, 2019.
• F. Chidichimo, M. R. Basile, C. Conidi, G. De Filpo, R. Morelli, and A. Cassano, "A new approach for bioremediation of olive mill wastewaters: combination of straw filtration and nanofiltration," Membranes, vol. 14, no. 2, p. 38, 2024.
• K. Ayadi, M. Meziane, D. Rouam, M. N. Bouziane, and K. El-Miloudi, "Olive mill wastewater for bioethanol production using immobilised cells," Kemija u industriji, vol. 71, no. 1-2, pp. 21–28, 2022.
• M. R. Zahi, W. Zam, and M. El Hattab, "State of knowledge on chemical, biological and nutritional properties of olive mill wastewater," Food Chemistry, vol. 381, p. 132238, 2022.
• G. J. Martin and S. Chan, "Future production of yeast biomass for sustainable proteins: a critical review," Sustainable Food Technology, 2024.
• D. I. Koukoumaki et al., "Recent advances in the production of single cell protein from renewable resources and applications," Carbon Resources Conversion, vol. 7, no. 2, p. 100195, 2024.
• M. Sharif et al., "Single cell protein: Sources, mechanism of production, nutritional value and its uses in aquaculture nutrition," Aquaculture, vol. 531, p. 735885, 2021.
• B. C. Bratosin, S. Darjan, and D. C. Vodnar, "Single cell protein: A potential substitute in human and animal nutrition," Sustainability, vol. 13, no. 16, p. 9284, 2021.
• P. Juszczyk, W. Rymowicz, A. Kita, and A. Rywińska, "Biomass production by Yarrowia lipolytica yeast using waste derived from the production of ethyl esters of polyunsaturated fatty acids of flaxseed oil," Industrial Crops and Products, vol. 138, p. 111590, 2019.
• R. Yang, Z. Chen, P. Hu, S. Zhang, and G. Luo, "Two-stage fermentation enhanced single-cell protein production by Yarrowia lipolytica from food waste," Bioresource Technology, vol. 361, p. 127677, 2022.
• K. Drzymała, A. M. Mirończuk, W. Pietrzak, and A. Dobrowolski, "Rye and oat agricultural wastes as substrate candidates for biomass production of the non-conventional yeast Yarrowia lipolytica," Sustainability, vol. 12, no. 18, p. 7704, 2020.
• M. Lopes et al., "The use of olive mill wastewater by wild type Yarrowia lipolytica strains: medium supplementation and surfactant presence effect," Journal of Chemical Technology & Biotechnology, vol. 84, no. 4, pp. 533–537, 2009.
• D. Sarris, M. Galiotou‐Panayotou, A. A. Koutinas, M. Komaitis, and S. Papanikolaou, "Citric acid, biomass and cellular lipid production by Yarrowia lipolytica strains cultivated on olive mill wastewater‐based media," Journal of Chemical Technology & Biotechnology, vol. 86, no. 11, pp. 1439–1448, 2011.
• D. Sarris et al., "Conversions of olive mill wastewater‐based media by Saccharomyces cerevisiae through sterile and non‐sterile bioprocesses," Journal of Chemical Technology & Biotechnology, vol. 88, no. 5, pp. 958–969, 2013.
• A. Cicci, M. Stoller, and M. Bravi, "Microalgal biomass production by using ultra-and nanofiltration membrane fractions of olive mill wastewater," Water Research, vol. 47, no. 13, pp. 4710–4718, 2013.
• G. Hodaifa, S. Sánchez, M. E. Martínez, and R. Órpez, "Biomass production of Scenedesmus obliquus from mixtures of urban and olive-oil mill wastewaters used as culture medium," Applied Energy, vol. 104, pp. 345–352, 2013.
• F. Di Caprio, P. Altimari, and F. Pagnanelli, "Integrated biomass production and biodegradation of olive mill wastewater by cultivation of Scenedesmus sp.," Algal Research, vol. 9, pp. 306–311, 2015.
• F. Arous et al., "Biosynthesis of single-cell biomass from olive mill wastewater by newly isolated yeasts," Environmental Science and Pollution Research, vol. 23, pp. 6783–6792, 2016.
• I. Giavasis and K. Petrotos, "Biovalorization of olive mill waste water for the production of single cell protein from Saccharomyces cerevisiae, Candida utilis, and Pleurotus ostreatus," British Biotechnology Journal, vol. 11, pp. 1–16, 2016.
• F. Tufaner, "Evaluation of COD and color removals of effluents from UASB reactor treating olive oil mill wastewater by Fenton process," Separation Science and Technology, vol. 55, no. 18, pp. 3455–3466, 2020.
• S. Papanikolaou et al., "Yarrowia lipolytica as a potential producer of citric acid from raw glycerol," Journal of Applied Microbiology, vol. 92, no. 4, pp. 737–744, 2002.
• D. Priyadarshi and K. K. Paul, "Optimisation of biodiesel production using Taguchi model," Waste and Biomass Valorization, vol. 10, pp. 1547–1559, 2019.
• M. S. Lucas and J. A. Peres, "Removal of COD from olive mill wastewater by Fenton’s reagent: Kinetic study," Journal of Hazardous Materials, vol. 168, no. 2-3, pp. 1253–1259, 2009.
• A. Jamrah, T. M. Al-Zghoul, and M. M. Darwish, "A comprehensive review of combined processes for olive mill wastewater treatments," Case Studies in Chemical and Environmental Engineering, vol. 100493, 2023.
• W. Cui et al., "Direct conversion of inulin into single cell protein by the engineered Yarrowia lipolytica carrying inulinase gene," Process Biochemistry, vol. 46, no. 7, pp. 1442–1448, 2011.
• M. C. Chalón et al., "Microbiological culture broth designed from food waste," Journal of Environmental Management, vol. 115, pp. 1–4, 2013.
• C. Zvidzai et al., "Potential commercialization of a microbial medium formulated from industrial food waste," African Journal of Microbiology Research, vol. 1, no. 6, pp. 079–087, 2007.
• E. Blanchet et al., "Comparison of synthetic medium and wastewater used as dilution medium to design scalable microbial anodes: application to food waste treatment," Bioresource Technology, vol. 185, pp. 106–115, 2015.
• T. L. Chen et al., "Implementation of green chemistry principles in circular economy system towards sustainable development goals: Challenges and perspectives," Science of the Total Environment, vol. 716, p. 136998, 2020.
• D. Sarris et al., "Production of added‐value metabolites by Yarrowia lipolytica growing in olive mill wastewater‐based media under aseptic and non‐aseptic conditions," Engineering in Life Sciences, vol. 17, no. 6, pp. 695–709, 2017.
• R. C. Carranza-Méndez et al., "Production of single cell protein from orange peel residues by Candida utilis," Biocatalysis and Agricultural Biotechnology, vol. 40, p. 102298, 2022.
• M. E. Jach et al., "Statistical evaluation of growth parameters in biofuel waste as a culture medium for improved production of single cell protein and amino acids by Yarrowia lipolytica," AMB Express, vol. 10, pp. 1–12, 2020.