Olfactory Bulb Lesion Induced Pineal Gland Calcification: a First Experimental Study

Abstract

Objectives: There is no enough stereological study that seriously mentions maternal olfactory disorders among the causes of pineal gland insults. This study sought to examine the connection between lesions caused by olfactory bulbectomy and changes in the pineal gland in rats Methods: A total of 24 male rats were used in the experiment, 5 control, 6 SHAM and 13 study. After a 1 mm burrhole was opened in the midfrontal-interpupillary line for the subjects in the SHAM and study groups, only the olfactory dura was opened in the SHAM group, while the olfactory bulbs were crushed in study group. They were sacrificed after a two-month follow-up. The number of calcified pineal cells (n/mm3) determined. Results analyzed with the Mann-Witney U test. Results: The mean calcified pineal cells numbers (n/mm3) were measured as per cubic milimeters as: (7±2) x103/mm3) in control (Group I); (15±3) x103 in SHAM (Group II); (34±9) x103 in study group (Group III). P<.005/(GI-GII); P<.0005/(GII-GIII); P<.00001/(GI-GIII). Conclusions: Olfactory bulb lesion may be responsible for pineal gland calcification. Pathologies in the pineal gland resulting from olfactory bulbectomy may contribute to various endocrine, immune, and reproductive disorders with unclear etiologies.

Keywords

• Olfactory Bulb Lesion
• Pineal Gland
• Calcification

Olfaktör Bulbus Lezyonu ile İndüklenmiş Pineal Gland Kalsifikasyonu: İlk Deneysel Çalışma

Abstract

Amaç: Pineal bez hastalıklarının nedenleri arasında maternal koku bozukluklarından ciddi şekilde bahseden yeterli stereolojik çalışma bulunmamaktadır. Bu çalışma, olfaktör bulbektominin neden olduğu lezyonlar ile sıçanlarda pineal bezdeki değişiklikler arasındaki bağlantıyı incelemeyi amaçlamıştır. Yöntem: Bu çalışmada 5 kontrol, 6 Sham ve 13 çalışma olmak üzere toplam 24 erkek sıçan kullanıldı. Sham ve çalışma grupları için midfrontal-interpupiller çizgide 1 mm'lik bir burrhole açıldıktan sonra, Sham grubunda sadece olfaktor dura açıldı, çalışma grubunda ise olfaktor bulbuslar hasarlandırılmıştır. İki aylık takipten sonra sıçanlar öldürüldüler. Kalsifiye edilmiş pineal hücre sayısı (n/mm3) belirlendi. Sonuçlar Mann-Witney U testi ile analiz edildi. Bulgular: Ortalama kalsifiye pineal hücre sayısı (n/mm3) milimetre küp başına şu şekilde ölçüldü: Kontrolde (Grup I) (7±2) x103/mm3); SHAM'da (Grup II) (15±3) x103); (34±9) x103 çalışma grubunda (Grup III). P<.005/(GI-GII); P<.0005/(GII-GIII); P<.00001/(GI-GIII). Sonuç: Olfaktör bulbus lezyonu pineal bez kalsifikasyonundan sorumlu olabilir. Olfaktor bulbektomi sonucu pineal bezde oluşan patolojiler etiyolojisi tam olarak bilinmeyen çeşitli endokrin, immün ve üreme bozukluklarına yol açabilir.

Keywords

• Olfaktör bulbus lezyonu
• pineal bez
• kalsifikasyon

References

1. Ferreira-Medeiros M, Mandarim-de-Lacerda CA, Correa-Gillieron EM. Pineal gland post-natal growth in rat revisited. Anat Histol Embryol. 2007;36(4):284-289. doi:10.1111/j.1439-0264.2007.00763.x
2. Nelson RJ, Moffatt CA, Goldman BD. Reproductive and nonreproductive responsiveness to photoperiod in laboratory rats. J Pineal Res. 1994;17(3):123-131. doi:10.1111/j.1600-079x.1994.tb00123.x
3. Cos S, Bardasano JL, Mediavilla MD, Sánchez Barceló EJ. [Synaptic ribbons in pinealocytes of olfactory bulbectomized rats under experimental conditions]. J Hirnforsch. 1989;30(1):91-98.
4. Gala RR, Clarke WP, Haisenleder DJ, Pan JT, Pieper DR. The influence of blinding, olfactory bulbectomy and pinealectomy on plasma and anterior pituitary prolactin levels and on uterine and anterior pituitary weights in normal and neonatally androgenized rats. Life Sci. 1985;36(17):1617-1624. doi:10.1016/0024-3205(85)90364-9
5. Leadem CA, Blask DE. Pineal gland inhibition of prolactin cell activity is independent of gonadal regression. Neuroendocrinology. 1982;35(2):133-138. doi:10.1159/000123368
6. Leadem CA, Blask DE. A comparative study of the effects of the pineal gland on prolactin synthesis, storage and release in male and female blind-anosmic rats. Biol Reprod. 1982;26(3):413-421. doi:10.1095/biolreprod26.3.413
7. Bukreeva I, Junemann O, Cedola A, et al. Micromorphology of pineal gland calcification in age-related neurodegenerative diseases. Med Phys. 2023;50(3):1601-1613. doi:10.1002/mp.16080
8. Lukac T, Matavulj A, Matavulj M, Rajković V, Lazetić B. Photoperiodism as a modifier of effect of extremely low-frequency electromagnetic field on morphological properties of pineal gland. Bosn J basic Med Sci. 2006;6(3):10-16. doi:10.17305/bjbms.2006.3136

9. Bittman EL, Crandell RG, Lehman MN. Influences of the paraventricular and suprachiasmatic nuclei and olfactory bulbs on melatonin responses in the golden hamster. Biol Reprod. 1989;40(1):118-126. doi:10.1095/biolreprod40.1.118
10. Sánchez-Criado JE, Guisado R. Corpus luteum progesterone secretion in rats undergoing pineal-mediated reproductive regression. J Pineal Res. 1988;5(5):489-497. doi:10.1111/j.1600-079x.1988.tb00791.x
11. Toma JG, Amerongen HM, Hennes SC, O’Brien MG, McBlain WA, Buzzell GR. Effects of olfactory bulbectomy, melatonin, and/or pinealectomy on three sublines of the Dunning R3327 rat prostatic adenocarcinoma. J Pineal Res. 1987;4(3):321-338. doi:10.1111/j.1600-079x.1987.tb00870.x
12. Pieper DR, Stone DA, Alexander RL, Subramanian MG. Olfactory bulbectomy prevents anti-gonadal effect of exogenous melatonin in male hamsters. Am J Physiol. 1986;251(6 Pt 2):R1208-11. doi:10.1152/ajpregu.1986.251.6.R1208
13. Leadem CA, Burns DM, Benson B. Possible involvement of the hypothalamic dopaminergic system in the prolactin-inhibitory effects of the pineal gland in blind-anosmic male rats. Neuroendocrinology. 1988;48(1):1-7. doi:10.1159/000124982
14. Pieper DR, Samyn MM, Heverly RN. Olfactory bulbectomy prevents short photoperiod-induced anestrus in female golden hamsters. Brain Res. 1986;380(1):54-58. doi:10.1016/0006-8993(86)91428-9
15. Mediavilla MD, Sánchez-Barceló EJ, Sánchez-Criado JE, Cos S, Cortines MD. Antigonadal actions of olfactory and light deprivation. II. Effects of pinealectomy or melatonin injections in olfactory bulb deafferented or bulbectomized male rats. J Pineal Res. 1985;2(2):191-200. doi:10.1111/j.1600-079x.1985.tb00639.x
16. Blask DE, Nodelman JL. An interaction between the pineal gland and olfactory deprivation in potentiating the effects of melatonin on gonads, accessory sex organs, and prolactin in male rats. J Neurosci Res. 1980;5(2):129-136. doi:10.1002/jnr.490050204
17. Leadem CA, Blask DE. Characterization of a pineal-mediated inhibition of pubertal prolactin cell development in blind-anosmic female rats. J Pineal Res. 1984;1(3):263-272. doi:10.1111/j.1600-079x.1984.tb00217.x
18. Kanat A, Aydin MD, Akca N, Ozmen S. First histopathological bridging of the distance between Onuf’s nucleus and substantia nigra after olfactory bulbectomy-new ideas about the urinary dysfunction in cerebral neurodegenerative disease: an experimental study. Low Urin Tract Symptoms. 2021;13(3):383-389. doi:10.1111/luts.12371
19. Olfactory Bulbectomy-Related Onuf’s Nucleus Degeneration: New Experimental Evidence for Kallmann Syndrome. Neuroendocrinology. 2023;113(10):1051-1058. doi:10.1159/000531962
20. Karadeniz E, Kocak MN, Ahiskalioglu A, et al. Exploring of the Unpredicted Effects of Olfactory Network Injuries on Mammary Gland Degeneration: A Preliminary Experimental Study. J Investig Surg Off J Acad Surg Res. 2019;32(7):624-631. doi:10.1080/08941939.2018.1446107
21. Firinci B, Caglar O, Karadeniz E, Ahiskalioglu A, Demirci T, Aydin MD. Mysterious effects of olfactory pathway lesions on intestinal immunodeficiency targeting Peyer’s patches: The first experimental study. Med Hypotheses. 2019;125:31-36. doi:10.1016/j.mehy.2019.02.032
22. Aydin N, Ramazanoglu L, Onen MR, et al. Rationalization of the Irrational Neuropathologic Basis of Hypothyroidism-Olfaction Disorders Paradox: Experimental Study. World Neurosurg. 2017;107:400-408. doi:10.1016/j.wneu.2017.07.180
23. Oral E, Aydin MD, Aydin N, et al. How olfaction disorders can cause depression? The role of habenular degeneration. Neuroscience. 2013;240:63-69. doi:10.1016/j.neuroscience.2013.02.026
24. Cos S, Garijo F, Corral J, Mediavilla MD, Sánchez-Barceló EJ. Histopathologic features of DMBA-induced mammary tumors in blind-underfed, blind-anosmic, and blind cold-exposed rats: influence of the pineal gland. J Pineal Res. 1989;6(3):221-231. doi:10.1111/j.1600-079x.1989.tb00418.x
25. Humbert W, Pévet P. The pineal gland of the aging rat: calcium localization and variation in the number of pinealocytes. J Pineal Res. 1995;18(1):32-40. doi:10.1111/j.1600-079x.1995.tb00137.x
26. Xerfan EMS, Morelhao PK, Arakaki FH, et al. Could melatonin have a potential adjuvant role in the treatment of the lasting anosmia associated with COVID-19? A review. Int J Dev Neurosci Off J Int Soc Dev Neurosci. 2022;82(6):465-470. doi:10.1002/jdn.10208