BIOCHEMICAL EVALUATIONS OF QUINALPHOS EXPOSED ZEBRAFISH LIVER ORGANOTYPIC TISSUE CULTURE

Abstract

As being a broad-spectrum, organophosphorus insecticide and acaricide, quinalphos is widely used against a range of pests and has a great threat to aquatic systems. Although the methods for detecting and predicting the harmful effects of chemicals on non-target organisms are traditionally perfected by in vivo experiments, cell culture methods that were used widely in recent decades are also an important tool for these kinds of research. In order to evaluate the effects of quinalphos on liver organotypic culture system of zebrafish, tissue cubes (1-2 mm3) were prepared by dissection and slicing of the liver tissues, embedded in agarose and cultured. The cubes were exposed to three different concentrations of quinalphos (2, 4 and 8 mg/L) for 24 and 96 hours. By performing the comet assay as an emerging tool for cytotoxicity, it was detected that quinalphos causes DNA damage. Increased levels of catalase, superoxide dismutase and glutathione-s-transferase were also measured. All of these parameters were noted as concentration- and time-dependent. Our data suggest that organotypic liver tissue culture of zebrafish is a practical alternative to the whole fish..

Keywords

• Zebrafish liver organotypic culture,quinalphos,oxidative stress

References

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