HISTOPATHOLOGICAL CHANGES IN THE STOMACH MUCOSA OF RATS FED WITH CARPET SHELL CLAM (RUDITAPES DECUSSATUS)

Year 2018, Volume: 2 Issue: 3, 115 - 124, 31.07.2018

Aslı Muratlı Mehmet Rıza Gezen

Abstract

Free radicals are produced in cells by cellular
metabolism and exogenous agents. These specie reacts with biomolecules in
cells, including DNA, proteins, lipids and carbonhydrates. The resulting
oxidative damage to DNA, is implicated in mutagenesis, carcinogenesis, and
aging. Heavy metals, industrial and household wastes and pesticides are threats
for the aquatic ecosystem. Polluted water sources are streaming into the seas
and cause pollution in these systems. Dardanelles is exposed to pollution from
the Marmara and Black Sea. Our previous studies demonstrated that the water and
mollusc from certain regions of the Dardanelles contained heavy metal salts.
The purpose of the study is to demonstrate DNA damage and the histopathologic
changes in the gastric tissues of rats which are fed with carpet shell clam
that are collected from the Çardak Lagoon (Çanakkale, Turkey). Four groups of
rats are included in the study, group 1 (n=6), control group fed with standard
rat food, group 2 (n=6), 75%  carpet
shell clam and 25% standard rat food daily, group 3 (n=6), 75% carpet shell
clam and 25% standard rat food every two days, group 4 (n=6), 75%  carpet shell clam and 25% standard rat food
every three days. After the routine histopathologic processing all gastric
tissue samples are evaluated in terms of 8-hydroxy-2' -deoxyguanosine (8-OHdG)
immunoreactivity with light microscopy and image analysis software. No histopathologic
differences found in standard hematoxylin-eosin stained gastric tissue samples
of the control group. Mucosal specimens of the second, third and fourth groups
showed mild mononuclear inflammation in favor of chronic gastritis. In immunohistochemical staining,
8-OHdG immunoreactivity in gastric epithelial cells.  8-OHdG immunoreactivity was negative in
stomach tissues in all groups.  There was
no statistically significant difference between the groups that were fed every
day, every other day and every three days with clam (p>0.05). The results of
our studies showed that rats fed more with carpet shell clam could produce
gastritis in the stomach

Keywords

Dardanelles, stomach, carpet shell clam, 8-hydroxy-2' -deoxyguanosine, immunohistochemistry

References

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