BIOCHEMICAL EVALUATIONS OF QUINALPHOS EXPOSED ZEBRAFISH LIVER ORGANOTYPIC TISSUE CULTURE

Year 2019, Volume: 3 Issue: 4, 319 - 328, 29.10.2019

Burak Gökçe , Sema İşisağ Üçüncü

https://doi.org/10.26900/jsp.3.033

Abstract

As being a broad-spectrum, organophosphorus insecticide and acaricide,
quinalphos is widely used against a range of pests and has a great threat to
aquatic systems. Although the methods for detecting and predicting the harmful
effects of chemicals on non-target organisms are traditionally perfected by in
vivo experiments, cell culture methods that were used widely in recent decades
are also an important tool for these kinds of research. In order to evaluate
the effects of quinalphos on liver organotypic culture system of zebrafish,
tissue cubes (1-2 mm3) were prepared by dissection and slicing of the liver
tissues, embedded in agarose and cultured. The cubes were exposed to three
different concentrations of quinalphos (2, 4 and 8 mg/L) for 24 and 96 hours.
By performing the comet assay as an emerging tool for cytotoxicity, it was
detected that quinalphos causes DNA damage. Increased levels of catalase,
superoxide dismutase and glutathione-s-transferase were also measured. All of
these parameters were noted as concentration- and time-dependent. Our data
suggest that organotypic liver tissue culture of zebrafish is a practical
alternative to the whole fish..

Keywords

Zebrafish liver organotypic culture, quinalphos, oxidative stress

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