Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri

Fikret Altındağ

Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri

Abstract

Amaç: Hepatotoksisite diyabetin en önemli sekonder komplikasyonlarından biridir. Diyabetin neden olduğu karaciğer hasarının ana nedenleri oksidatif stres ve inflamasyondur. Sinapik asidin güçlü bir antioksidan ve antiinflamatuar olduğu öne sürülmüştür. Bu çalışmada, deneysel diyabet modelinde sinapik asidin (SA) hepatoprotektif etkilerini TNF-α, AST, ALT seviyeleri, MT boyama yoğunluğu ve histolojik değişiklikleri değerlendirerek araştırmayı amaçladık. Materyal ve Metot: Sıçanlar dört gruba ayrıldı (n=7): Sham (S), SA, Diyabetik (D), Diyabetik+Sinapik Asit (D+SA). S grubuna intragastrik (i.g.) yolla serum fizyolojik verildi. SA grubuna 28 gün boyunca i.g. yolla 20 mg/kg/gün SA verildi. D grubuna tek doz 50 mg/kg STZ intraperitoneal (i.p.) enjekte edildi. D+SA grubuna, tek doz 50 mg/kg STZ i.p. yolla enjekte edildi ve 28 gün boyunca 20 mg/kg/gün SA i.g. yolla verildi. Tümor nekroz faktör-alfa (TNF-α) ekspresyonu immünohistokimyasal yöntemle değerlendirildi. Karaciğerde olası fibrozisi değerlendirmek için Masson’s trichrome (MT) boyası ve histolojik inceleme için hematoksilen-eozin (H-E) boyası ile karaciğer boyandı. Ayrıca karaciğer fonksiyon testleri olan serum AST ve ALT seviyeleri ölçüldü. Bulgular: S ve SA grupları normal histolojik mimariye ve negatif TNF-a immüno-ekspresyonuna sahipti. S grubu ile karşılaştırıldığında, D grubu daha yüksek AST ve ALT seviyelerine ve MT boyama yoğunluğuna sahipti. D grubunda şiddetli TNF-α immün ekpresyonunun yanı sıra vasküler dilatasyon, apoptotik hücreler ve inflamatuar hücrelerin infiltrasyonu gibi histopatolojik değişiklikler gözlendi. D grubu ile karşılaştırıldığında D+SA grubunda TNF-α immunoexpression, histopatolojik değişiklikler, AST ve ALT seviyeleri azaldı. Sonuç: Çalışmamız, STZ ile indüklenen diyabetik sıçanlarda SA’nın hepatotoksisiteye karşı hepatoprotektif bir etkiye sahip olabileceğini ortaya koydu.

Keywords

References

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Details

Primary Language

Turkish

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Fikret Altındağ This is me
Türkiye

Publication Date

December 1, 2021

Submission Date

September 16, 2021

Acceptance Date

December 9, 2021

Published in Issue

Year 2021 Volume: 11 Number: 3

IZ

https://izlik.org/JA43LC57SY

Cite

RIS / Bibtex

APA

Altındağ, F. (2021). Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri. Kafkas Journal of Medical Sciences, 11(3), 410-416. https://izlik.org/JA43LC57SY

AMA

1.Altındağ F. Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri. Kafkas Journal of Medical Sciences. 2021;11(3):410-416. https://izlik.org/JA43LC57SY

Chicago

Altındağ, Fikret. 2021. “Streptozotosin Ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri”. Kafkas Journal of Medical Sciences 11 (3): 410-16. https://izlik.org/JA43LC57SY.

EndNote

Altındağ F (December 1, 2021) Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri. Kafkas Journal of Medical Sciences 11 3 410–416.

IEEE

[1]F. Altındağ, “Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri”, Kafkas Journal of Medical Sciences, vol. 11, no. 3, pp. 410–416, Dec. 2021, [Online]. Available: https://izlik.org/JA43LC57SY

ISNAD

Altındağ, Fikret. “Streptozotosin Ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri”. Kafkas Journal of Medical Sciences 11/3 (December 1, 2021): 410-416. https://izlik.org/JA43LC57SY.

JAMA

1.Altındağ F. Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri. Kafkas Journal of Medical Sciences. 2021;11:410–416.

MLA

Altındağ, Fikret. “Streptozotosin Ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri”. Kafkas Journal of Medical Sciences, vol. 11, no. 3, Dec. 2021, pp. 410-6, https://izlik.org/JA43LC57SY.

Vancouver

1.Fikret Altındağ. Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Sinapik Asidin Hepatoprotektif Etkileri. Kafkas Journal of Medical Sciences [Internet]. 2021 Dec. 1;11(3):410-6. Available from: https://izlik.org/JA43LC57SY