The Effects of Engeletin on Cell Proliferation and Invasion in the Human Breast Cancer Cell Line (MCF-7)

Abstract

Aim: Belonging to the group of flovonoids, Engeletin is a molecule with strong anti-inflammatory, antioxidant and anticancer properties. However, the effect of this molecule on breast cancer cells has not been studied yet. For this purpose, the effectiveness of Engeletin (ENG) on cell proliferation, invasion and apoptosis in the human breast cancer cell line (MCF-7) was investigated in this study, Materials and Methods: ENG was studied at 1, 10 and 100 µM doses in the MCF-7 cell line. In the study, cell proliferation was analyzed by MTT cell viability test, its effectiveness on invasion was analyzed by transwell assay, cellular viability and apoptotic evaluation were analyzed by fluorescence staining method. Results: It was determined that Engeletin reduced MCF-7 cell proliferation. The ENG 100 µM dose was found to be the most effective dose. While ENG application decreases the number of viable cells, it causes an increase in the number of apoptotic cells. In addition, it was determined that ENG application significantly decreased the number of invasive cells in a dose-dependent manner compared to the control group (p<0.001). Conclusion: Engeletin is a molecule with anti-carcinogenic, antiproliferative activity on MCF-7 cells. In addition, ENG shows an anti-invasive activity in MCF-7 cells, demonstrating that it is a molecule with anti-metastatic activity.

Keywords

• Engeletin
• Cell Proliferation
• Cell Viability
• Invasion and MCF-7

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