Methylation of GLUT5 and Electromotile Responses During Chronic and Acute Sodium Salicylate Administration in the Cochlear Outer Hair Cells

Abstract

Aim: Prestin molecule and transporter fructose GLUT5 in the lateral walls of outer hair cells are essential. This study aimed to investigate epigenetic alterations in the GLUT5 gene. Materials and methods: The animals were divided into three groups randomly. No injection was received in the first group (n=3). Groups 2(n=3) and 3(n=6) were injected with intramuscular saline and sodium salicylate, respectively. Electrophysiological measurements were performed at 1st, 2nd, and 8th hours to evaluate the acute effect and in 2 weeks to evaluate the chronic effect. Methylation of CpG dinucleotides in the promoter can lead to dysregulated and repressed gene expression. Genomic DNA was isolated from bone tissues, treated with bisulfite, and analyzed using methylation specific PCR (polymerase chain reaction) (MSP). Epigenetic alterations in the GLUT5 gene were investigated by using the MSP method. Results: There was a significant decrease in electrophysiological measurements at all frequencies in the acute effect (p<0.01), whereas there was no significant difference in the chronic effect for Group 3 (p>0.05). However, methylation of GLUT5 was observed to be increased during acute administration, followed by a decreasing trend to normal during the chronic period. Conclusion: Our findings show that methylation of GLUT5 may decrease GLUT5 expression in lateral walls of outer hair cells, thereby changing prestin-bound fructose transport in cell membranes due to reduced GLUT5 expression.

Keywords

• GLUT5
• outer hair cells
• cochlea
• distortion product otoacoustic emissions; sodium salicylate

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