Evaluation of HLA-B*51 Subtypes in Behçet's Patients with Uveitis

Year 2017, , 193 - 196, 01.12.2017

Eda Balkan Nilnur Eyerci Sadullah Keleş Orhan Ateş Hasan Doğan İbrahim Pirim Aslı Kara

https://doi.org/10.5505/kjms.2017.39306

Abstract

Aim: The HLA-B*51 allele has been determined to be the most
important genetic factor in the pathogenesis of Behçet’s disease
(BD). This relationship has been demonstrated in various ethnic
groups and many studies have shown sequence alterations in
B*51 protein coding regions. To date, 116 different subtypes of
HLA-B*51 (HLA-B*51:01-B*51:122) have been identified (IMG/
HLA 3.5.0, June 14, 2011). This study investigated the distribution
of B*51 subtypes in patients diagnosed with BD according to
the 1990 International Study Group criteria and positive for B*51
compared to healthy controls.
Material and Method: DNA was isolated from 40 unrelated B*51-
positive BD patients and 54 healthy volunteer bone marrow donors.
B*51 subtype analysis was done by polymerase chain reaction
with sequence specific primers (PCR-SSP) (One Lambda Inc.,
CA, USA). Chi-square and Fisher’s exact tests were used in the
statistical analysis (SPSS version 17.0).
Results: There were no statistically significant associations between
B*51 subtype and BD patients’ clinical characteristics or laboratory
parameters (p<0.05). No significant difference was found between
BD patients and controls in the frequency of B*51 subtypes.
Conclusion: Although there has been much emphasis on the
association between BD and the HLA*5101 subtype, which is a
common finding in BD patients in the Turkish population and in
other ethnic groups, the presence of this subtype at a comparable
frequency in the control group indicates that the development of
BD is not attributable to HLA*5101 alone. Our data suggest that in
addition to genetic factors, certain environmental factors also play
a role in the development of BD

Keywords

Behçet’s disease, uveitis, allele, HLA-B*51, PCR-SSP

Behçet Üveitli Hastalarda HLA-B*51 Alt Tipinin Değerlendirilmesi

Abstract

Aim: The HLA-B*51 allele has been determined to be the most
important genetic factor in the pathogenesis of Behçet’s disease
(BD). This relationship has been demonstrated in various ethnic
groups and many studies have shown sequence alterations in
B*51 protein coding regions. To date, 116 different subtypes of
HLA-B*51 (HLA-B*51:01-B*51:122) have been identified (IMG/
HLA 3.5.0, June 14, 2011). This study investigated the distribution
of B*51 subtypes in patients diagnosed with BD according to
the 1990 International Study Group criteria and positive for B*51
compared to healthy controls.
Material and Method: DNA was isolated from 40 unrelated B*51-
positive BD patients and 54 healthy volunteer bone marrow donors.
B*51 subtype analysis was done by polymerase chain reaction
with sequence specific primers (PCR-SSP) (One Lambda Inc.,
CA, USA). Chi-square and Fisher’s exact tests were used in the
statistical analysis (SPSS version 17.0).
Results: There were no statistically significant associations between
B*51 subtype and BD patients’ clinical characteristics or laboratory
parameters (p<0.05). No significant difference was found between
BD patients and controls in the frequency of B*51 subtypes.
Conclusion: Although there has been much emphasis on the
association between BD and the HLA*5101 subtype, which is a
common finding in BD patients in the Turkish population and in
other ethnic groups, the presence of this subtype at a comparable
frequency in the control group indicates that the development of
BD is not attributable to HLA*5101 alone. Our data suggest that in
addition to genetic factors, certain environmental factors also play
a role in the development of BD

Keywords

Behçet hastalığı, üveit, allel, HLA-B*51, PCR-SSP

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