Deneysel Sisplatin Nefrotoksisitesi Modelinde Sesamolün Etkileri

Abstract

Amaç: Sisplatin böbrekte oksidatif hasara neden olmaktadır. Sesamol, susam yağının suda çözünür ve antioksidan bileşenlerinden biridir. Sesamolün, deneysel sisplatin nefrotoksisitesi ve lipid peroksidasyonu üzerindeki etkisini araştırdık. Materyal ve Metot: Yirmi sekiz erkek, Wistar albino rat dört gruba ayrıldı (n=7): konrol, sesamol (8 mg/kg/gün), sisplatin (7 mg/kg i.p., tek doz), ve sisplatin + sesamol. Örnekler biyokimyasal, moleküler ve histopatolojik olarak değerlendirildi. İstatistiksel analiz için SPSS paket program kullanıldı. Bulgular: Üre ve kreatinin düzeyleri sisplatin grubunda kontrol grubuna göre istatistiksel olarak anlamlı yüksek bulunurken, sisplatin+sesamol grubunda ise anlamlı düşüş görüldü (p<0,05). Sisplatin verilen ratlarda malondialdehid (MDA) düzeylerinde anlamlı artış saptandı (p<0,05). Sesamol, MDA düzeylerini anlamlı ölçüde azalttı (p<0,05). Nükleer faktör kappa B (NF-κB) düzeyleri sisplatin grubunda kontrol grubuna göre istatistiksel olarak anlamlı yüksek bulunurken, sisplatin+sesamol grubunda ise anlamlı düşüş görüldü (p<0,05). Nükleer faktör eritroid 2-ilişkili faktör 2 (Nrf2) ve hem oksijenaz-1 (HO-1) düzeyleri sisplatin grubunda kontrol grubuna göre istatistiksel olarak anlamlı düşük (p<0,001) bulunurken, sisplatin+sesamol grubunda ise anlamlı yükselme görüldü (p<0,01). Sisplatinin neden olduğu histopatolojik değişikliklerin, sesamol uygulamasıyla azaldığı görüldü. Sonuç: Sesamolün, sisplatin nedenli nefropatide lipid peroksidasyonu ve NF-κB düzeylerini azalttığını ve antioksidan etki göstererek Nrf2 ve HO-1 düzeylerini artırdığını saptadık.

Keywords

• sisplatin
• nefrotoksisite
• sesamo
• nükleer faktör kappa B
• nükleer faktör eritroid 2-ilişkili faktör 2
• hem oksijenaz-1

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