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Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-carbohydrate and High-lipid Diets

Year 2013, Yıl: 2013 Sayı: 1, 1 - 7, 01.03.2013

Abstract

AIM: The objective of the present study was to evaluate the effects
of green tea extract and Lactobacillus casei strain Shirota on
levels of serum minerals (Ca, Cu, Zn, Fe, Mg and Mn), cholesterol,
triglyceride, glucose and lactate in rats on high carbohydrate and
lipid diets.
METHODS: Thirty fi ve healthy Wistar albino rats were used, fi ve in
each of seven experimental groups: control (group A), high-carbohydrate
diet (group B), high-carbohydrate diet supplemented with
probiotic bacteria for 8 weeks - Lactobacillus casei strain Shirota
(group C), high-carbohydrate diet supplemented with green tea
extract for 8 weeks - in drinking water: 100 mg/kg/day (group D),
high-lipid diet for 8 weeks (group E), high-lipid diet supplemented
with probiotic bacteria for 8 weeks (group F), high-lipid diet supplemented
with green tea extract for 8 weeks- in drinking water:
100 mg/kg/day (group G).
RESULTS: A signifi cant increase (p< 0.05) in serum cholesterol
was observed in groups treated with high-lipid, and high-lipid +
green tea extracts (E and G) compared with other groups (P< 0.05).
Serum triglyceride levels were signifi cantly lower in the high-carbohydrate
+ probiotic group (C) than in other groups (p< 0.05).
High-calorie diet, green tea extract and probiotic bacteria had no
infl uence on serum lactate levels in any of the groups. Serum Ca
levels decreased signifi cantly only in the high-carbohydrate diet
group (p< 0.05). However, serum Zn and Fe concentrations increased
signifi cantly in the high-lipid and high-lipid plus probiotic
bacteria groups, respectively (p< 0.05 and 0.01). Serum Mg levels
were signifi cantly lower in all experimental groups compared to
the control group (P< 0.01). L. casei strain Shirota and the green
tea extract, signifi cantly lowered serum glucose levels in the highcarbohydrate
groups compared to high-lipid groups (p< 0.05).
CONCLUSION: The green tea extract and L. casei strain Shirota
decreases serum glucose and triglyceride levels in rats fed on
high-calorie diets.

Yeșil Çay Ekstraktı ve Lactobacillus Casei Strain Shirota’nın Yüksek Karbonhidrat ve Lipit İçerikli Diyetle Beslenen Ratlarda Serum Mineral, Kolesterol, Trigliserid, Glikoz ve Laktat Seviyeleri Üzerine Etkileri

Year 2013, Yıl: 2013 Sayı: 1, 1 - 7, 01.03.2013

Abstract

AMAÇ: Bu çalıșmanın amacı, yeșil çay ekstraktı ve Lactobacillus
casei strain Shirota’nın yüksek karbonhidrat ve lipit içerikli diyetle
beslenen ratlarda serum mineralleri (Ca, Cu, Zn, Fe, Mg and Mn),
kolesterol, trigliserid, glikoz ve laktat düzeyleri üzerine etkilerinin
araștırılmasıdır.
YÖNTEM: Otuzbeș sağlıklı Wistar albino rat her grupta 5 hayvan olacak
șekilde 7 gruba ayrıldı: Kontrol grubu (A), yüksek-karbonhidratlı
diyet grubu (B), yüksek karbonhidratlı diyet ve 8 hafta süreyle probiyotik
bakteri ilaveli grup- Lactobacillus casei strain Shirota (C), yüksek
karbonhidratlı diyet ve 8 hafta süreyle yeșil çay ekstraktı verilen grup
(D), yüksek lipit içerikli diyet grubu (E), yüksek lipit içerikli diyet ve 8
hafta süreyle probiyotik bakteri ilaveli grup- Lactobacillus casei strain
Shirota (F) ve yüksek lipit içerikli diyet ve 8 hafta süreyle yeșil çay
ekstraktı ilaveli grup (G).
BULGULAR: Serum kolesterol seviyesinin yüksek lipit ve yüksek
lipit + yeșil çay ekstraktı ilaveli gruplarda (grup E ve G) kontrole
ve diğer deneme gruplarına göre anlamlı olarak artığı tespit edildi
(p< 0.05). Serum trigliserid seviyesi yüksek karbonhidrat + probiyotik
ilaveli grupta (grup C) diğer deneme gruplarından anlamlı olarak
düșüktü (p< 0.05). Yüksek kalorili diyet, yeșil çay ekstraktı ve probiyotik
ilavesinin gruplarda serum laktat seviyesi üzerine herhangi
bir etkisinin olmadığı görüldü. Serum Ca düzeyinin yanlızca yüksek
karbonhidrat içerikli diyet grubunda anlamlı olarak azaldığı tespit
edildi (p< 0.05). Bununla birlikte, serum Zn ve Fe konsantrasyonları-
nın yüksek lipit ve yüksek lipit + probiyotik ilaveli gruplarda sırasıyla
p< 0.05 ve P< 0.01 düzeylerinde anlamlı olarak artığı tespit edildi.
Serum Mg seviyesinin tüm deneme gruplarında kontrole göre istatistiksel
olarak anlamlı derecede azaldığı tespit edildi (P< 0.01). L.
casei strain Shirota ve yeșil çay ekstraktı alınması yüksek karbonhidrat
diyetli gruplarda serum glikoz seviyesini yüksek lipid diyetli
gruba göre anlamlı olarak azalttığı belirlendi (p< 0.05).
SONUÇ: Yeșil çay ekstraktı ve L. casei strain Shirota kullanımının
yüksek enerjili diyetle beslenen ratlarda kan glukoz ve trigliserid seviyelerini
düșürücü etkisi vardır.

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Details

Primary Language English
Subjects Clinical Sciences
Other ID JA39GE63PA
Journal Section Research Article
Authors

Turan Karaca This is me

Fahri Bayıroğlu This is me

Mustafa Cemek This is me

Bahat Comba

Ahmet Ayaz This is me

İhsan Karaboğa This is me

Publication Date March 1, 2013
Published in Issue Year 2013 Yıl: 2013 Sayı: 1

Cite

APA Karaca, T., Bayıroğlu, F., Cemek, M., Comba, B., et al. (2013). Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-carbohydrate and High-lipid Diets. Kafkas Journal of Medical Sciences(1), 1-7.
AMA Karaca T, Bayıroğlu F, Cemek M, Comba B, Ayaz A, Karaboğa İ. Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-carbohydrate and High-lipid Diets. KAFKAS TIP BİL DERG. March 2013;(1):1-7.
Chicago Karaca, Turan, Fahri Bayıroğlu, Mustafa Cemek, Bahat Comba, Ahmet Ayaz, and İhsan Karaboğa. “Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-Carbohydrate and High-Lipid Diets”. Kafkas Journal of Medical Sciences, no. 1 (March 2013): 1-7.
EndNote Karaca T, Bayıroğlu F, Cemek M, Comba B, Ayaz A, Karaboğa İ (March 1, 2013) Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-carbohydrate and High-lipid Diets. Kafkas Journal of Medical Sciences 1 1–7.
IEEE T. Karaca, F. Bayıroğlu, M. Cemek, B. Comba, A. Ayaz, and İ. Karaboğa, “Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-carbohydrate and High-lipid Diets”, KAFKAS TIP BİL DERG, no. 1, pp. 1–7, March 2013.
ISNAD Karaca, Turan et al. “Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-Carbohydrate and High-Lipid Diets”. Kafkas Journal of Medical Sciences 1 (March 2013), 1-7.
JAMA Karaca T, Bayıroğlu F, Cemek M, Comba B, Ayaz A, Karaboğa İ. Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-carbohydrate and High-lipid Diets. KAFKAS TIP BİL DERG. 2013;:1–7.
MLA Karaca, Turan et al. “Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-Carbohydrate and High-Lipid Diets”. Kafkas Journal of Medical Sciences, no. 1, 2013, pp. 1-7.
Vancouver Karaca T, Bayıroğlu F, Cemek M, Comba B, Ayaz A, Karaboğa İ. Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-carbohydrate and High-lipid Diets. KAFKAS TIP BİL DERG. 2013(1):1-7.