Gossypin’in L929 Fibroblast Hücrelerindeki Hidrojen Peroksit Hasarına Karşı Koruyucu Etkilerinin Değerlendirilmesi

Year 2020, Volume: 10 Issue: 1, 15 - 23, 01.04.2020

İrfan Çınar

Abstract

Amaç: Bu çalışmada, fare L929 fibroblast hücrelerinde H2O2kaynaklı oksidatif stresi önlemede ve yara iyileşme modelinde
Gossypin’in kapasitesini değerlendirdik. Materyal ve Metot: Gossypin’in (5–200 µg/mL) ve H2O2’nin (0,3–0,9mM) in-vitro yara iyileşme aktivitesi, hücre canlılığı ve sitotoksisite indeksleri MTT tahlili kullanılarak fare fibroblast hücre hattı L929’da değerlendirildi. Çalışmada, in-vitro yara iyileşme testi modeli olarak migrasyon testi yapıldı. Antioksidan aktivitenin değerlendirilmesi amacı ile H2O2’nin (0,5 mM) konsantrasyonu kullanıldı. SOD aktivitesi ve MDA seviyesi belirlendi. Ayrıca Gossypin dozlarının H2O2’nin neden olduğu hasarı azaltıp azaltamadığını değerlendirmek için, gerçek zamanlı PCR kullanarak L929 hücrelerinde IL-1β ve TNF-α mRNA
ekspresyon seviyelerini analiz ettik. Bulgular: H2O2 (0,3–0,9 mM) uygulamasının doz bağımlı olarak, Gossypin’in (5–200 µg/mL) ise 75 µg/mL’den sonra L929 hücrelerine proliferasyonu inhibe ettiği belirlenmiştir. Gossypin’in 25 ve 50 µg/mL dozları H2O2 (0,5 mM) uygulamasına karşı 48. ve 72. saatlerde L929 hücrelerini korumuştur. Bu sonuçlar migrasyon testi ile doğrulanmıştır. Gossypin uygulanması, antioksidan aktivitesini önemli ölçüde arttırmış, MDA oluşumunu azaltmıştır.
Özellikle 50 μg/mL grubu ve H2O2 grubuna kıyasla SOD’u arttırmıştır. Gossypin dozları değerlendirilen H2O2 grubuna kıyasla IL-1β ve TNF-α mRNA ekspresyonunu anlamlı şekilde azaltmıştır. Sonuç: Bu çalışmada, L929 hücrelerinde H2O2 tarafından indüklenen oksidatif stresinden koruyarak fibroblast canlılığı ve migrasyonu üzerinde güçlü bir etki göstermiştir. Bu, Gossypin’in H2O2’nin neden olduğu oksidatif stres ile ilişkili hastalıklarının önlenmesinde potansiyel etkisini ortaya koymaktadır. Buda cilt dokusunun H2O2’nin neden olduğu oksidatif stres benzeri hasarlanmalara bağlı hastalıkların tedavisinde Gossypin’in kullanılabileceğini göstermektedir.

Keywords

Gossypin, L929, H2 O2 ; oksidatif stres; yara iyileşmesi

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