Cytotoxic Effects of Helichrysum arenarium on HepG2 Cells

Year 2025, Volume: 15 Issue: 2, 254 - 259, 31.08.2025

Duygu Kırkık , Derya Sultan Karabulut Sevgi Kalkanlı Taş

Abstract

Aim: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. The search for natural compounds with potential anticancer properties has gained increasing attention. Helichrysum arenarium is a medicinal plant known for its bioactive compounds, but its cytotoxic effects on liver cancer cells remain unexplored. This study aimed to evaluate the cytotoxic effects of Helichrysum arenarium extract on HepG2 liver cancer cells by assessing cell viability and morphological changes.
Material and Method: HepG2 cells were treated with different concentrations (0.025–0.1 mg/mL) of Helichrysum arenarium extract for 24 hours. Morphological alterations were observed using phase-contrast microscopy, while cell viability was assessed using the WST-1 assay. Statistical analysis was performed using oneway ANOVA with Tukey’s post-hoc test, considering p <0.05 as statistically significant.
Results: Helichrysum arenarium exhibited a dose-dependent cytotoxic effect on HepG2 cells. Morphological observations indicated apoptotic-like changes, and the WST-1 assay showed a significant decrease in cell viability, with the highest concentration (0.1 mg/mL) reducing viability to below 30% of the control (p <0.05).
Conclusion: These findings suggest that Helichrysum arenarium exhibits potent cytotoxic effects on HepG2 liver cancer cells, likely through potential apoptotic pathways. Further studies are needed to elucidate the molecular pathways involved and to evaluate its potential as a therapeutic agent for hepatocellular carcinoma.

Keywords

Helichrysum arenarium , HepG2 cell line , hepatocellular carcinoma , cytotoxicity; medicinal p

References

• 1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394–424.
• 2. Burley MR, Roth CM. Effects of retinoic acid on proliferation and differentiation of HepG2 cells. Open Biotechnol J. 2017;1:47–51.
• 3. Pljevljakušić D, Bigović D, Janković T, Jelačić S, Šavikin K. Sandy everlasting (Helichrysum arenarium (L.) Moench): Botanical, chemical and biological properties. Front Plant Sci. 2018;9:1123.
• 4. Akinyede KA, Cupido CN, Hughes GD, Oguntibeju OO, Ekpo OE. Medicinal properties and in vitro biological activities of selected Helichrysum species from South Africa: a review. Plants. 2021;10(8):1566.
• 5. Czinner E, Kéry A, Hagymási K, Blázovics A, Lugasi A, Szöke E, et al. Biologically active compounds of Helichrysum arenarium (L.) Moench. Eur J Drug Metab Pharmacokinet. 1999;24(4):309–13.
• 6. Judzentiene A, Budiene J, Nedveckyte I, Garjonyte R. Antioxidant and toxic activity of Helichrysum arenarium (L.) Moench and Helichrysum italicum (Roth) G. Don essential oils and extracts. Molecules. 2022;27(4):1311.
• 7. Tanker N, Sezik G. Researches on species of Helichrysum growing in Turkey, from the point of view of pharmaceutical botany. J Fac Pharm Ankara Univ. 1978;8(1):19–39.
• 8. Thusyanthan J, Wickramaratne NS, Senathilake KS, Rajagopalan U, Tennekoon KH, Thabrew I, et al. Cytotoxicity against human hepatocellular carcinoma (HepG2) cells and anti-oxidant activity of selected endemic or medicinal plants in Sri Lanka. Adv Pharmacol Pharm Sci. 2022;2022:6407688.
• 9. Ranke J, Mölter K, Stock F, Bottin-Weber U, Poczobutt J, Hoffmann J, et al. Biological effects of imidazolium ionic liquids with varying chain lengths in acute Vibrio fischeri and WST-1 cell viability assays. Ecotoxicol Environ Saf. 2004;58(3):396404.
• 10. Cheng YC, Chang JM, Chen CA, Chen HC. Autophagy modulates endoplasmic reticulum stress-induced cell death in podocytes: a protective role. Exp Biol Med (Maywood). 2015;240(4):467–76.
• 11. Tabatabaei FS, Moezizadeh M, Javand F. Effects of extracts of Salvadora persica on proliferation and viability of human dental pulp stem cells. J Conserv Dent. 2015;18(4):315–20.
• 12. Samant H, Amiri HS, Zibari GB. Addressing the worldwide hepatocellular carcinoma: epidemiology, prevention and management. J Gastrointest Oncol. 2021;12(Suppl 2):S361S373.
• 13. Nalkiran I, Sevim Nalkiran H. Phytochemical profile and anticancer potential of Helichrysum arenarium extracts on glioblastoma, bladder cancer, and breast cancer cells. Pharmaceuticals (Basel). 2025;18(2):144.
• 14. Asma ST, Acaroz U, Imre K, Morar A, Shah SRA, Hussain SZ, et al. Natural products/bioactive compounds as a source of anticancer drugs. Cancers (Basel). 2022;14(24):6203.
• 15. Mansuri ML, Parihar P, Solanki I, Parihar MS. Flavonoids in modulation of cell survival signalling pathways. Genes Nutr. 2014;9(3):400.
• 16. Zhang HW, Hu JJ, Fu RQ, Liu X, Zhang YH, Li J, et al. Flavonoids inhibit cell proliferation and induce apoptosis and autophagy through downregulation of PI3Kγ mediated PI3K/ AKT/mTOR/p70S6K/ULK signaling pathway in human breast cancer cells. Sci Rep. 2018;8(1):11255