DROSOPHİLA MELANOGASTER’İN SOMATİK MUTASYON VE REKOMBİNASYON TESTİ İLE DELTAMETRİN VE PERMETRİNİN GENOTOKSİK AKTİVİTESİNİN BULUNMASI

Öz

Bu çalışmada pretiroid insektisitlerden deltametrin ve organofosfatlardan permetrinin Drosophila melanogaster’de genotoksik etkileri Somatik Mutasyon ve Rekombinasyon testi ile analiz edildi. Kullanılan kimyasalların letal dozları belirlendi. İşaret genleri taşıyan mwh ve flr3 transheterozigot larvaları insektisitlerin 25 ppm, 50 ppm and 75 ppm konsantrasyonları için değerlendirildi. Toplam mutasyon, mutasyon gözlenen kanat sayısı ve yaşama yüzdesi arasında pozitif korelasyon gözlendi. Gözlenen mutasyonlar kanat başına mutasyon oluşturan hücre sayısına ve tipine göre sınıflandırıldı ve veriler istatistiksel analiz ile değerlendirildi. İki pestisit arasından deltametrinin permetrinden daha toksik ve mutajenik olduğu gözlendi.

Anahtar Kelimeler

• Drosophila melanogaster,somatik mutasyon ve rekombinasyon test,insektisit,genotoksik etki

THE DETECTION OF GENOTOXIC ACTIVITY OF THE DELTAMETHRIN AND PERMETHRIN BY SOMATIC MUTATION AND RECOMBINATION TEST WITH DROSOPHILA

Abstract

In this study, different doses of deltamethrin from pyretyroide insecticides and permethrine from organophosphate insecticides have been analysied for their genotoxic effects by somatic mutation and recombination test SMART in Drosophila melanogaster. Lethal doses of chemicals used were determined. Trans-heterozygous larvae for two genetic markers mwh and flr3 were evaluated for 25 ppm, 50 ppm and 75 ppm concentrations of the insecticides. A possitive correlation was observed among total mutations, the number of wings having mutations and percentage survival. Mutations observed were classified according to their size and type per wing and the data evaluated by statistical analysis. Deltamethrin was found more toxic and mutagenic than permethrine

Keywords

• Drosophila melanogaster,somatic mutation and recombination test,insecticides,genotoxic effect

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