Microbial Characterization of Streptomyces Isolates from Oil Contaminated Soil
Abstract
In this study, nineteen different Streptomyces strains were isolated from oil-contaminated surface soil at Ataş Oil Terminal in Turkey. Biodegradation and Restriction Fragment Length Polymorphism tests (RFLP) were used for selecting isolates. The isolates belonging to the genus Streptomyces were confirmed by 16S rDNA analysis. 1592r, 1492r, 800r, 518f, and 27f primers were chosen for the 16S rDNA gene amplifying and sequencing. Then 93-unit characters were used to determine phenotypic, physiological, and biochemical properties. All strains can degrade Tween 80, casein, gelatin, hypoxanthine, RNA, L-Tyrosine, elastin. Nevertheless, EL039, EL045, and EL060 soil isolates have better degradation potentials because of gave positive results to eleven degradation tests. According to the obtained results, EL037, EL039, EL060 could be a member of S. albogriseolus, S. rochei, S. mutabilis, respectively, and the phylogenetic tree and characterization tests indicated that especially, EL038, EL045, EL057 could be new members of Streptomyces genus. We perceive that the Streptomyces isolates would benefit biotechnological studies thanks to the degradation enzyme potentials.
Keywords
EcoRI, PstI, HindP1I, biodegradation
Supporting Institution
Ondokuz Mayıs University
Project Number
F-473
Thanks
16S rDNA analysis supported financially by Ondokuz Mayıs University Project No: F-473, Turkey.
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