Microbial Characterization of Streptomyces Isolates from Oil Contaminated Soil

Year 2022, , 545 - 559, 15.12.2022

Elif Çil , Kamil Işık

https://doi.org/10.31466/kfbd.1012606

Abstract

In this study, nineteen different Streptomyces strains were isolated from oil-contaminated surface soil at Ataş Oil Terminal in Turkey. Biodegradation and Restriction Fragment Length Polymorphism tests (RFLP) were used for selecting isolates. The isolates belonging to the genus Streptomyces were confirmed by 16S rDNA analysis. 1592r, 1492r, 800r, 518f, and 27f primers were chosen for the 16S rDNA gene amplifying and sequencing. Then 93-unit characters were used to determine phenotypic, physiological, and biochemical properties. All strains can degrade Tween 80, casein, gelatin, hypoxanthine, RNA, L-Tyrosine, elastin. Nevertheless, EL039, EL045, and EL060 soil isolates have better degradation potentials because of gave positive results to eleven degradation tests. According to the obtained results, EL037, EL039, EL060 could be a member of S. albogriseolus, S. rochei, S. mutabilis, respectively, and the phylogenetic tree and characterization tests indicated that especially, EL038, EL045, EL057 could be new members of Streptomyces genus. We perceive that the Streptomyces isolates would benefit biotechnological studies thanks to the degradation enzyme potentials.

Keywords

EcoRI, PstI, HindP1I, biodegradation

Supporting Institution

Ondokuz Mayıs University

Project Number

F-473

Thanks

16S rDNA analysis supported financially by Ondokuz Mayıs University Project No: F-473, Turkey.

Petrolle Kirlenmiş Topraktan Elde Edilen Streptomyces İzolatlarının Mikrobiyal Karakterizasyonu

Abstract

Bu çalışmada, Türkiye'de Ataş Petrol Terminali'nde yağla kirlenmiş yüzey toprağından on dokuz farklı Streptomyces suşu izole edilmiştir. İzolatların seçiminde Biyobozunma ve Restriksiyon Parça Uzunluk Polimorfizmi (RFLP) testleri kullanılmıştır. Streptomyces cinsine ait izolatlar, 16S rDNA analizi ile doğrulanmıştır. 16S rDNA geni amplifikasyonu ve dizilemesi için 1592r, 1492r, 800r, 518f ve 27f primerleri seçilmiştir. Ardından fenotipik, fizyolojik ve biyokimyasal özellikleri belirlemek için 93 birimlik karakterler kullanılmıştır. Tüm suşlar Tween 80, kazein, jelatin, hipoksantin, RNA, L-tirozin, elastin'i parçalayabilmiştir. Bununla birlikte, EL039, EL045 ve EL060 toprak izolatlarının, on bir bozunma testine de pozitif sonuç verdiğinden daha iyi bozunma potansiyellerine sahip olduğu düşünülmektedir. Elde edilen sonuçlara göre EL037, EL039, EL060 sırasıyla S. albogriseolus, S. rochei, S. mutabilis'in bir üyesi olabilir ve filogenetik ağaç ve karakterizasyon testleri özellikle EL038, EL045, EL057'nin yeni üye olabileceğini göstermiştir. Streptomyces cinsine aittir. Streptomyces izolatlarının degradasyon enzim potansiyelleri sayesinde biyoteknolojik çalışmalara fayda sağlayacağı kanaatindeyiz.

Keywords

EcoRI, PstI, HindP1I, biyobozunma

Project Number

F-473

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