Biogenic Synthesis of Silver and Iron Oxide Nanoparticles Using Aronia prunifolia Leaf Extract and Its Inhibitory Action Against Pathogenic Fungi

Luau Mustafa; Ahmed Ismael Naqee Al-bayatı; Dunya Albayati; İbrahim Özkoç

doi:10.31466/kfbd.1399112

EN TR

Biogenic Synthesis of Silver and Iron Oxide Nanoparticles Using Aronia prunifolia Leaf Extract and Its Inhibitory Action Against Pathogenic Fungi

Abstract

Because of the environment and abundant renewable resources, exploiting plant extracts to form metallic nanoparticles has become a promising alternative to chemical and physical methods. Numerous studies have shown that nanoparticles of silver (AgNPs) and iron oxide (Fe2O3NPs) have inhibitory effects against pathogenic fungi. In this study, we used the leaf extract of Aronia prunifolia to generate biogenic AgNPs and FeNPs, aiming to demonstrate the impact of nanoparticles on pathogenic fungi. Nanoparticles are characterized by UV-Vis, X-ray diffraction, EDX spectrum, and SEM techniques. Leaf extracts used for nanosynthesis yielded silver and iron oxide nanoparticles with distinct color changes and absorption peaks, showcasing tetragonal, pentagonal, and hexagonal shapes (15-50 nm) for silver and spherical morphology (16-60 nm) for iron oxide. The antifungal effectiveness of nanoparticles against Aspergillus fumigatus, Rhizoctonia solani Ag4 HgII, and Aspergillus flavus was investigated using a well diffusion method. Inhibition zones, ranging from 12.5 to 35.0 mm for AgNPs and 7.1 to 17.1 mm for FeNPs at concentrations of 10 to 30 µg/ml respectively, demonstrated the superior inhibitory potential of AgNPs over FeNPs. This study aims to address a gap in the literature by examining the inhibitory effects of green AgNPs and FeNPs on pathogenic fungi. Encased nanoparticles can be very useful in treating fungal infections; this will be the first investigation into the production of nanoparticles from A. prunifolia leaves.

Keywords

Well diffusion method , Nanoparticle characterization , Aronia prunifolia , Green synthesis , Antifungal activity

Aronia prunifolia Yaprağı Ekstresi Kullanılarak Gümüş ve Demir Oksit Nanopartiküllerinin Biyojenik Sentezi ve Patojenik Funguslara Karşı İnhibitör Etkisi

Öz

Çevre ve yenilenebilir kaynakların bolluğu nedeniyle, metalik nanopartiküller oluşturmak için bitki özlerinden yararlanmak, kimyasal ve fiziksel yöntemlere umut verici bir alternatif haline geldi. Çok sayıda çalışma, gümüş (AgNP'ler) ve demir oksit (Fe2O3NP'ler) nanopartiküllerinin patojenik funguslara karşı önleyici etkiler olduğunu göstermiştir. Bu çalışmada Aronia prunifolia'nın yaprak ekstraktı kullanılarak biyojenik AgNP'ler ve FeNP'ler üretilerek yaprak ekstraktı ve nanopartiküllerin patojenik funguslar üzerindeki etkileri gösterilmiştir. Nanopartiküller UV-Vis, X-ışını kırınımı, EDX spektrumu ve SEM teknikleriyle karakterize edilmiştir. Nanosentez için kullanılan yaprak ekstraktları, gümüş için dörtgen, beşgen ve altıgen şekiller (15-50 nm) ve demir oksit için küresel morfoloji (16-60 nm) sergileyen, farklı renk değişimleri ve absorpsiyon zirveleri olan gümüş ve demir oksit nanopartikülleri verdi. Nanopartiküllerinin Aspergillus fumigatus, Rhizoctonia solani Ag4 HgII ve Aspergillus flavus'a karşı antifungal aktivitesi, iyi bir difüzyon yöntemi kullanılarak incelenmiştir. 10 ila 30 µg/ml konsantrasyonlarda AgNP'ler için 12.5 ila 35.0 mm ve FeNP'ler için 7.1 ila 17.1 mm arasında değişen inhibisyon bölgeleri, AgNP'lerin FeNP'lere göre üstün inhibitör potansiyelini ortaya koydu. Bu çalışmada, yeşil AgNP'ler ve FeNP'nin patojen fungus izolüne karşı önleyici aktivitesini karşılaştırarak literatürdeki bir boşluğu doldurmayı umuyoruz. Kaplanmış nanopartiküller fungus enfeksiyonlarının tedavisinde çok faydalı olabilir; bu, A. prunifolia yapraklarından nanopartiküllerin üretimine yönelik ilk araştırma olacak.

Anahtar Kelimeler

: Well difüzyon yöntemi , Nanopartikül karakterizasyonu , Aronia prunifolia , Yeşil sentez , Antifungal aktivite

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Luau Mustafa ^*
0000-0001-8197-4357
Iraq

Ahmed Ismael Naqee Al-bayatı
0000-0001-5411-7448
Türkiye

Dunya Albayati
0009-0006-9152-2323
Iraq

İbrahim Özkoç
0000-0001-8179-0961
Türkiye

Publication Date

June 18, 2024

Submission Date

December 1, 2023

Acceptance Date

May 29, 2024

Published in Issue

Year 2024 Volume: 14 Number: 2

DOI

https://doi.org/10.31466/kfbd.1399112

IZ

https://izlik.org/JA85JN85GP

APA

Mustafa, L., Al-bayatı, A. I. N., Albayati, D., & Özkoç, İ. (2024). Biogenic Synthesis of Silver and Iron Oxide Nanoparticles Using Aronia prunifolia Leaf Extract and Its Inhibitory Action Against Pathogenic Fungi. Karadeniz Fen Bilimleri Dergisi, 14(2), 589-604. https://doi.org/10.31466/kfbd.1399112

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