Endemik Onosma nana DC.’nin Kimyasal Bileşimi, Antimikrobiyal, Antioksidan ve Enzim İnhibisyon Aktivitesinin Araştırılması

Year 2024, , 982 - 996, 18.06.2024

Kadriye Özcan , Tuba Acet

https://doi.org/10.31466/kfbd.1472704

Abstract

Türkçe adı “Emzik otu” olarak bilinen Onosma türleri, etnobotanik açıdan oldukça değerlidir. Bu çalışmada, Onosma nana’nın toprak üstü ve kök kısımlarının farklı çözücülerle (etanol, metanol ve etil asetat) elde edilen ekstrelerinin antioksidan, antimikrobiyal, enzim inhibisyon (α-amilaz, α-glukozidaz ve tirozidaz) ve DNA koruyucu aktivitesi ile fenolik bileşen analizinin (HPLC) yapılması amaçlanmıştır. Elde edilen sonuçlara göre, etanol ekstrelerinin diğer ekstrelere nazaran araştırılan özellikler bakımından daha etkin olduğu bulunmuştur. Etanol ekstrelerinin major fenolik bileşeni rosmarinik asit (kök: 2883.3 μg/g ekstre, toprak üstü: 11187.5 μg/g ekstre) olarak tespit edilmiştir. Sonuç olarak, bitkinin oldukça etkili antimikrobiyal aktiviteye sahip olduğu bulunmuştur (S. epidermidis ve P. vulgaris: MİK 32µg/mL). Bitkinin DNA koruyucu etkisi ve antimikrobiyal özellikleri ilk defa araştırılmıştır. Bitkinin doğal bir ajan olarak kullanılma potansiyeli olduğu söylenebilir. Bunun yanısıra, tespit edilen aktivitelerin detaylı çalışmalarla desteklenerek etki mekanizmalarının aydınlatılması ticari öneminin anlaşılması bakımından gereklidir.

Keywords

Antimikrobiyal, Enzim inhibisyon, Antioksidan, Fenolik içerik, Onosma nana, HPLC

Supporting Institution

Giresun Üniversitesi Bilimsel Araştırma Projeleri

Project Number

FEN-BAP-A-240222-25

Thanks

Bu çalışma, Giresun Üniversitesi Bilimsel Araştırma Projeleri (Proje no: FEN-BAP-A-240222-25) tarafından desteklenmiştir.

Investigation of Chemical Composition, Antimicrobial, Antioxidant and Enzyme Inhibition Activity of Endemic Onosma nana DC.

Abstract

Onosma species, known as “Emzik otu” in Turkish, are very valuable in terms of ethnobotany. In this study, it was aimed to analyze the antioxidant, antimicrobial, enzyme inhibition (α-amylase, α-glucosidase and tyrosidase) and DNA protective activities and phenolic compounds (HPLC) of the extracts obtained from the aboveground and root parts of Onosma nana with different solvents (ethanol, methanol and ethyl acetate). According to the results obtained, ethanol extracts were found to be more effective than other extracts in terms of the investigated properties. The major phenolic component of ethanol extracts was rosmarinic acid (root: 2883.3 μg/g extract, aboveground: 11187.5 μg/g extract). As a result, the plant was found to have highly effective antimicrobial activity (S. epidermidis and P. vulgaris: MIC 32µg/mL). The DNA protective effect and antimicrobial properties of the plant have been investigated for the first time. It can be said that the plant has the potential to be used as a natural agent. In addition, it is necessary to elucidate the mechanisms of action by supporting the detected activities with detailed studies in order to understand its commercial importance.

Keywords

Antimicrobial, Enzyme inhibition, Antioxidant, Phenolic content, Onosma nana, HPLC

Project Number

FEN-BAP-A-240222-25

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