The Role of Flavonoids in the Antimicrobial Activity of Peganum harmala Extract

Didem Verep Koç; Aytaç Güder; Kerim Güney; Mahmut Gür; Saim Ateş

doi:10.31466/kfbd.1557101

EN TR

The Role of Flavonoids in the Antimicrobial Activity of Peganum harmala Extract

Abstract

The use of plant extracts and their active compounds for medicinal purposes, particularly for antimicrobial activity, has become increasingly prevalent in recent years. This study aimed to evaluate the antimicrobial activities of Peganum harmala seed extract against various microorganisms using the disc diffusion method. Ethanol (65% concentration) was used to extract the active components from the P. harmala plant. Eight types of flavonoids in the plant extract were analyzed through HPLC. The antimicrobial activity of the extract was assessed against 15 microorganisms, including 14 bacterial strains and 1 fungus, via disc diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) tests. The results indicated that P. harmala exhibited activity against Bacillus subtilis, Candida albicans, Enterobacter aerogenes, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas fluorescens, Salmonella enteritidis, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium, Staphylococcus aureus, and Staphylococcus epidermidis, with MIC values ranging from 6.50 µg/mL to 52.03 µg/mL. The P. harmala extract demonstrated both antibacterial and antifungal activity against all tested microorganisms, with varying efficacy.

Keywords

Peganum harmala , Antimicrobial activity , Flavonoid , Disc diffusion , MIC , MBC-MFC

Üzerlik Ekstresinin Antimikrobiyal Aktivitesinde Flavonoidlerin Rolü

Öz

Bitki ekstraktlarının ve bunların aktif bileşiklerinin tıbbi amaçlarla, özellikle antimikrobiyal aktivite için kullanımı son yıllarda giderek yaygınlaşmaktadır. Bu çalışmada Üzerlik tohumu ekstraktının çeşitli mikroorganizmalara karşı antimikrobiyal aktivitelerinin disk difüzyon yöntemi kullanılarak değerlendirilmesi amaçlandı. Aktif bileşenlerin P. harmala bitkisinden ekstrakte edilmesi için etanol (%65 konsantrasyon) kullanıldı. Bitki ekstraktındaki sekiz tip flavonoid HPLC yoluyla analiz edildi. Ekstraktın antimikrobiyal aktivitesi, 14 bakteri suşu ve 1 mantar dahil 15 mikroorganizmaya karşı disk difüzyon, minimum inhibitör konsantrasyon (MIC), minimum bakterisidal konsantrasyon (MBC) ve minimum fungisidal konsantrasyon (MFC) testleri yoluyla değerlendirildi. Sonuçlar P. harmala'nın Bacillus subtilis, Candida albicans, Enterobacter aerogenes, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas fluorescens, Salmonella enteritidis, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium'a karşı aktivite gösterdiğini gösterdi. MİK değerleri 6,50 µg/mL ile 52,03 µg/mL arasında değişen Staphylococcus aureus ve Staphylococcus epidermidis. P. harmala ekstraktı, test edilen tüm mikroorganizmalara karşı değişen etkinlikle hem antibakteriyel hem de antifungal aktivite gösterdi.

Anahtar Kelimeler

Peganum harmala , Antimikrobiyal aktivite , Flavonoid , Disk difüzyon , MİK , MBK-MFK

References

Abdulridha, M.M., Abdulhussein, H.S., Alyaseen, F.F., and Hassan, B.A. (2019). Phytochemical and Antibacterial Activity of the Peganum harmala Seeds and Its Alkaloids. Plant Archives. 19:1439-1444.
Aburjai, T., Hudaib, M., Tayyem, R., Yousef, M., and Qishawi, M. (2007). Ethnopharmacological survey of medicinal herbs in Jordan, the Ajloun Heights region. Journal of Ethnopharmacology. 110:294–304.
Ait Abderrahim, L.A., Taibi, K., and Abderrahim, C.A. (2019). Assessment of the Antimicrobial and Antioxidant Activities of ziziphus lotus and Peganum harmala. Iran J. Sci. Technol. Trans Sci. 43:409-414.
Allaq, A.A., Sidik, N.J., Abdul-Aziz, A., and Ahmed, I.A. (2021). Antioxidant, antibacterial and phytochemical secreening of ethanolic crude extracts of Libyan Peganum harmala seeds. Journal of Pharmaceutical Research International. 33(13):74-82.
Altuner, E.M. (2011). Investigation of antimicrobial activity of Punica granatum L. fruit peel ash used for protective against skin infections as folk remedies especially after male circumcision. African Journal of Microbiology Research. 5(20):3339-3342.
Altuner, E.M., and Canlı, K. (2012). In vitro antimicrobial screening of Hypnum andoi A.J.E. Sm. Kastamonu U. J. For. Fac. 12:97-101.
Altuner, E.M., Akata, I., and Canlı, K. (2012). In vitro antimicrobial screening of Bovista nigrescens (Pers.). Kastamonu U. J. For. Fac. 12:90-96.
Altuner, E.M., Canlı, K., and Akata, I. (2014). Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme. Journal of Pure and Applied Microbiology. 8(1):539-545.
Ansari, M,N. (2020). Assessment of Antidiarrheal, Antispasmodic and Antimicrobial Activities of Mmethanolic Seeds Extract of Peganum harmala L. (Nitrariaceae). Journal of Pharmaceutical Research International. 32(24):74-82.
Arif, M., Wang, X., Khan Kazi, M.S., Khan, S.U., Saeed, S., Khan, A.M., et al. (2022). Antimicrobial Activities of different solvent extracts from stem and seeds of Peganum harmala L. Plos One. 17:4.

Arsdall, V.A. (2023). Medieval Herbal Remedies: The Old English Herbarium and Early-medieval Medicine. Routledge.
Balouiri, M., Sadiki, M., Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis. 6(2):71-79.
Borneo, R., Le´on, A.E., Aguirre, A., Ribotta, P., and Cantero, J.J. (2009). Antioxidant capacity of medicinal plants from the Province of C´ordoba (Argentina) and their in vitro testing in a model food system. Food Chem. 70:112:664.
Canlı, K., Altuner, E.M., and Akata, I. (2015). Antimicrobial screening of Mnium stellare. Bangladesh Journal of Pharmacology. 10:321-325.
Canlı, K., Altuner, E.M., Akata, I., Türkmen, Y., and Üzek, U. (2016). In vitro antimicrobial screening of Lycoperdon lividium and determination of the ethanol extract composition by gas chromatography/mass spectrometry. Bangladesh Journal of Pharmacology. 11(2):389-394.
Dönmez, İ.E., Hemming, J., and Willför, S. (2016). Bark Extractives and Suberin Monomers from Arbutus Andrachne and Platanus Orientalis. BioRes. 11(1):2809-2819.
Dubey, R.K., Upadhyay, G., Singh, V., and Pandey, S. (2020). Antioxidant potential and free radical scavenging activity of Parkia roxburghii, G. Don, a lesser known leguminous tree from North East India, South African Journal of Botany. 131:454-461.
Essawi, T., and Srour, M. (2000). Screening of Some Palestinian Medicinal Plants for Antibacterial Activity. Journal of Ethnoparmacology. 70:343-349.
Estivi, L., Buratti, S., Fusi, D., Benedetti, S., Rodríguez, G., Brandolini, A., & Hidalgo, A. (2022). Alkaloid content and taste profile assessed by electronic tongue of Lupinus albus seeds debittered by different methods. Journal of Food Composition and Analysis, 114, 104810.
Gazzaneo, L.R., de Lucena, R.F.P., and de Albuquerque, U.P. (2005). Knowledge and use of medicinal plants by local specialists in a region of Atlantic Forest in the state of Pernambuco (Northeastern Brazil). Journal of Ethnobiology and Ethnomedicine. 1:9.
Hadadi, Z., Nematzadeh, G.A., and Ghahari, S. (2020). A study on the antioxidant and antimicrobial activities in the chloroformic and methanolic extracts of 6 important medicinal plants collected from North of Iran. BMC Chemistry. 14:33.
Hajji, A., Bnejdi, F., Saadoun, M., Ben Salem, I., Nehdi, I., Sbihi, H., Alharthi, F. A., El Bok, S., and Boughalleb-M'Hamdi, N. (2020). High reserve in δ-tocopherol of Peganum harmala seeds oil and antifungal activity of oil against ten plant Pathogenic fungi. Molecules. 25:4569.
Iranshahy, M., Bazzaz, S.F., Haririzadeh, G., Abootorabi, B.Z., Mohamadi, A.M., and Khashyarmanesh, Z. (2019). Chemical composition and antibacterial Properties of Peganum harmala L.. Avicenna Journal of Phytomedicine. 9(6):530-537.
Kaya, E., and Akbaş, P. (2023). Determination of the Antimicrobial, Antioxidant Activities and Fatty Acid Composition of Peganum harmala Seed. Brazilian Archives of Biology and Technology. 66:23220237.
Koçak, Y., and Şahin, A. (2009). Peganum harmala L. (üzerlik) tohum ekstresinin analjezik aktivitesi ve akut toksisitesinin fareler üzerinde belirlenmesi. Y.Y.U. Veteriner Fakultesi Dergisi. 20:27-30.
Krakhmalnyi, M., Isikhuemhen, O. S., Jeppson, M., Wasser, S. P., & Nevo, E. (2023). Species Diversity of Lycoperdaceae (Agaricales) in Israel, with Some Insights into the Phylogenetic Structure of the Family. Journal of Fungi, 9(10), 1038.
Loizzo, M.R., Tundis, R., Chandrika, U.G., Abeysekera, A.M., Menichini, F., and Frega, N.G. (2010). Antioxidant and antibacterial activities on foodborne pathogens of Artocarpus heterophyllus Lam. (Moraceae) leaves extracts. J Food Sci. 75:291–5.
Mahmoud, S.S., and Jassim, E.H. (2021). Antibacterial activity of alkaloids extract from Peganum harmala L. seeds against Staphylococcus aureus and Klebsılla pneumonia isolated from burns. Plant Archives. 21:951-957.
Makhlof, M. E., El-Sheekh, M. M., & El-Sayed, A. I. (2024). In vitro antibiofilm, antibacterial, antioxidant, and antitumor activities of the brown alga Padina pavonica biomass extract. International Journal of Environmental Health Research, 34(4), 1861-1878.
Maksimovic, Z.A., Dordeviv, S., and Mraovic, M. (2005). Antimicrobial activity of chenopodium botrys essential oil. Fitoterapia. 76:112-114.
Mangalagiri, N. P., Panditi, S. K., & Jeevigunta, N. L. L. (2021). Antimicrobial activity of essential plant oils and their major components. Heliyon, 7(4).
Mizuno, M., and Nishitani, Y. (2013). Chapter Bioactive Food as Dietary Interventions for Liver and Gastrointestinal Disease, 14 - Antioxidant, Luteolin Exhibits Anti-inflammatory Effect in In Vitro Gut Inflammation Model, ISSN: 978-0-12-397154-8 Pages 227-234, https://doi.org/10.1016/C2011-0-07464-1
Palchykov, V.A., Zazharskyi, V.V., Brygadyrenko, V.V., Davydenko, P.O., Kulishenko, O.M., and Borovik, I.V. (2020). Chemical composition and antibacterial effect of ethanolic extract of Buxus sempervirens on cryogenic strains of microorganisms in vitro. Chemical Data Collections. 25:100323.
Saeedeh, F., Oryan, S., Ahmadi, R., and Eidi, A. (2022). Evaluation of chemical components, Antioxidant Properties and lethal toxicity of alkaloids extracted from espand (Peganum harmala). Journal of Applied Biological Sciences. 16(2):257-265.
Satari, A., Ghasemi, S., Habtemariam, S., Asgharian, S., & Lorigooini, Z. (2021). Rutin: A flavonoid as an effective sensitizer for anticancer therapy; insights into multifaceted mechanisms and applicability for combination therapy. Evidence‐Based Complementary and Alternative Medicine, 2021(1), 9913179.
Shaheen, G., Ashfad, A., Shamim, T., Asif, H.M., Ali, A., Rehman, S., and Sumreen, L. (2022). Antioxidant, Antimicrobial, Phytochemical and FTIR Analysis of Peganum harmala (Fruit) Ethanolic Extract from Cholistan Desert, Pakistan. Dose-Response: An International Journal. 1-7.
Shahrajabian, M.H., Sun, W., and Cheng, Q. (2021). Improving health benefits with considering traditional and modern health benefits of Peganum harmala. Clinical Phytoscience. 7:18.
Shukla, R., Pandey, V., and Lodhi, S. (2019) Chapter 18 - Role of Flavonoids in Management of Inflammatory Disorders, Pages 293-322, Book: Bioactive Food as Dietary Interventions for Arthritis and Related Inflammatory Diseases, ISSN:978-0-12-813820-5 Elsevier Inc. All.
Şener, İ., Gür, M., Verep, D., Güney, K., and Altuner, E. M. (2017). Antimicrobial Activities and Some Flavonoids in Extracts of Some Medicinal Plants Indian Journal of Pharmaceutical Education and Research. 51:234-238.
Tosun, B. (2024). Introduction to cultivation Angelica sylvestris var. sylvestris L.: Chemical composition of essential oil and antimicrobial activity. Journal of Essential Oil Bearing Plants, 27(4), 1175-1189.
Yuca, H., Aydın, B., Tekman, E., Göger, G., Karakaya, S., Güvenalp, Z., and Ayşe Mine, G. Ö. (2022). Peganum harmala L. (Nitrariaceae) bitkisinin α-amilaz ve α-glukozidaz enzim inhibisyon, antioksidan ve antimikrobiyal aktivitelerinin değerlendirilmesi. Türk Tarım ve Doğa Bilimleri Dergisi. 9(4):925-932.

Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Didem Verep Koç
0000-0002-1013-9212
Türkiye

Aytaç Güder ^*
0000-0002-1190-8749
Türkiye

Kerim Güney
0000-0003-2305-790X
Türkiye

Mahmut Gür
0000-0001-9942-6324
Türkiye

Saim Ateş
0000-0003-0589-1773
Türkiye

Publication Date

December 15, 2024

Submission Date

September 27, 2024

Acceptance Date

December 9, 2024

Published in Issue

Year 2024 Volume: 14 Number: 4

DOI

https://doi.org/10.31466/kfbd.1557101

IZ

https://izlik.org/JA52TT72KX

APA

Verep Koç, D., Güder, A., Güney, K., Gür, M., & Ateş, S. (2024). The Role of Flavonoids in the Antimicrobial Activity of Peganum harmala Extract. Karadeniz Fen Bilimleri Dergisi, 14(4), 2244-2257. https://doi.org/10.31466/kfbd.1557101

The published articles in The Black Sea Journal of Sciences are licensed under Creative Commons Attribution-NonCommercial 4.0 International

The Role of Flavonoids in the Antimicrobial Activity of Peganum harmala Extract

Abstract

Keywords

Üzerlik Ekstresinin Antimikrobiyal Aktivitesinde Flavonoidlerin Rolü

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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