DPPH (1,1-diphenyl-2-picrylhydrazyl) Free Radical Sweeping Activity of Scenedesmus sp.
Abstract
Spectrophotometric DPPH method was used for the determination
of DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity of Scenedesmus sp. which was isolated from
the Giresun mine and cultivated under BG11 culture conditions. 1 mL of 10-3 M
DPPH ethanol solution was added and 3 mL of extract solution was added and
vortexed. The absorbance was read at 517 nm for 30 minutes in the dark. BHT and
Trolox were used as standard antioxidants. The DPPH radical scavenging activity
reaction was calculated using the formula.
Keywords
References
- Akyıl S., İlter I., Koç M. ve Kaymak-Ertekin F. 2016. Alglerden elde edilen yüksek değerlikli biyoaktif/Biyolojik Uygulama Alanları Akademik Gıda, 14 (4) : 418-423.
- Brand-Williams, W., Cuvelier M. E. ve Berset C. 1994. Use of free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft & Technologie, 28: 25-30.
- Britton G. 1995. Structure and properties of carotenoids in relation of function. FASEB J., 9:1551–1558.
- El-Sheekh M. M., Osman M. E. H., Dyab M. A. ve Amer M. S. 2006. Production and characterization of antimicrobial active substance from the Cyanobacterium Nostoc muscorum. Environmental Toxicology and Pharmacology, 21(1): 42–50.
- Fagali N. ve Catalá A. 2008. Antioxidant activity of conjugated linoleic acid isomers, linoleic acid and its methyl ester determined by photoemission and DPPH radical dot techniques. Biophys. Chem., 137: 56–62.
- Guedes A., Amaro H. M. ve Malcata F. X. 2011. Microalgae as sources of high added‐value compounds a brief review of recent work. Biotechnology Progress, 27(3): 597-613.
- Halim R., Danquah M. K., Webley P. A. 2012. Extraction of oil from microalgae for biodiesel production: A review. Biotechnol. Adv., 30: 709–732.
- Herrero M., Cifuentes A. Ve Ibañez E. 2006. Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food by-products, algae and microalgae—A review. Food Chem., 98:136–148.
- Tandeau-de-Marsac N. ve Houmard J. 1993. Adaptation of cyanobacteria to environmental stimuli: New steps towards molecular mechanisms. FEMS Microbiol. Rev., 104: 119–190.
- León R., Martín M., Vigara J., Vilchez C. ve Vega J. 2003. Microalgae-mediated photoproduction of β-carotene in aqueous organic two-phase systems. Biomol. Eng., 20: 177–182.
