Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması

Kadriye Özcan

doi:10.31466/kfbd.563428

TR EN

Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması

Abstract

Bu çalışmada, Yıldız Gölü (Gümüşhane) sedimentinden aktinobakteri izolasyonu gerçekleştirilmiş ve izolatlar antimikrobiyal aktivite ve enzim üretme kapasiteleri (amilaz, lipaz, proteaz, pektinaz, selülaz) bakımından incelenmiştir. Tarama, uygun besiyeriler içeren petrilerde gerçekleştirilmiştir. İzolasyon için SCA besiyeri kullanılmış ve besiyeriye nistatin ve nalidiksik asit ilave edilmiştir. Antimikrobiyal aktivite tespiti için, çapraz çizgi ekim yöntemi ve C. tropicalis, C. albicans, E. coli, P. aeruginosa, MRSA, E. faecium, E. fecalis standart suşları tercih edilmiştir. Sonuç olarak izolatların %55’i en az bir test mikroorganizmasına karşı antimikrobiyal aktivite gösterirken %66.6’sının ise en az bir enzimi üretebildiği belirlenmiştir. Bununla birlikte izolatların %22.2’si ne antimikrobiyal aktivite ne de enzim üretme yeteneğine sahip bulunmuştur. Bu çalışma Yıldız Gölü’nden aktinobakteri izolasyonu ve izolatların biyolojik aktivitesi üzerine gerçekleştirilen ilk araştırmadır. Sonuç olarak, elde edilen aktinobakterilerin endüstriyel ve farmakolojik çalışmalar için kaynak olabileceği öngörülmekle birlikte aktif bileşiklerin izolasyonu ve karakterizasyonu için ileri çalışmalara ihtiyaç duyulmaktadır

Keywords

Antimikrobiyal aktivite,Enzim üretimi,İzolasyon,Tarama

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Details

Primary Language

Turkish

Subjects

Engineering

Journal Section

Research Article

Authors

Kadriye Özcan ^*
0000-0002-4913-6035
Türkiye

Publication Date

June 30, 2019

Submission Date

May 12, 2019

Acceptance Date

June 3, 2019

Published in Issue

Year 2019 Volume: 9 Number: 1

DOI

https://doi.org/10.31466/kfbd.563428

IZ

https://izlik.org/JA53AU65MP

APA

Özcan, K. (2019). Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması. Karadeniz Fen Bilimleri Dergisi, 9(1), 144-151. https://doi.org/10.31466/kfbd.563428

AMA

1.Özcan K. Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması. KFBD. 2019;9(1):144-151. doi:10.31466/kfbd.563428

Chicago

Özcan, Kadriye. 2019. “Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal Ve Enzim Üretim Kapasitelerinin Araştırılması”. Karadeniz Fen Bilimleri Dergisi 9 (1): 144-51. https://doi.org/10.31466/kfbd.563428.

EndNote

Özcan K (June 1, 2019) Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması. Karadeniz Fen Bilimleri Dergisi 9 1 144–151.

IEEE

[1]K. Özcan, “Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması”, KFBD, vol. 9, no. 1, pp. 144–151, June 2019, doi: 10.31466/kfbd.563428.

ISNAD

Özcan, Kadriye. “Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal Ve Enzim Üretim Kapasitelerinin Araştırılması”. Karadeniz Fen Bilimleri Dergisi 9/1 (June 1, 2019): 144-151. https://doi.org/10.31466/kfbd.563428.

JAMA

1.Özcan K. Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması. KFBD. 2019;9:144–151.

MLA

Özcan, Kadriye. “Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal Ve Enzim Üretim Kapasitelerinin Araştırılması”. Karadeniz Fen Bilimleri Dergisi, vol. 9, no. 1, June 2019, pp. 144-51, doi:10.31466/kfbd.563428.

Vancouver

1.Kadriye Özcan. Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması. KFBD. 2019 Jun. 1;9(1):144-51. doi:10.31466/kfbd.563428

Cited By

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https://doi.org/10.31466/kfbd.889423

Determination of Pathogenicity of Bacteria Isolated from Some Aquatic Amphibian and Reptile Species

Biology Bulletin

https://doi.org/10.1134/S1062359024604725

The published articles in The Black Sea Journal of Sciences are licensed under Creative Commons Attribution-NonCommercial 4.0 International

Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması

Abstract

Keywords

Investigation of Antimicrobial and Enzyme Production Capacities of Actinobacteria Isolated from Yıldız Lake Sediment

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Sakarya Nehir Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Bitki Gelişim Teşvik Edici Özelliklerinin Belirlenmesi

Determination of Pathogenicity of Bacteria Isolated from Some Aquatic Amphibian and Reptile Species